LncRNA MIR503HG is downregulated in Han Chinese with colorectal cancer and inhibits cell migration and invasion mediated by TGF-β2

Chuo, Dongyu; Liu, Fang; Chen, Yuze; Yin, Mingdi

doi:10.1016/j.gene.2019.143960

Cited by 19 publications

(17 citation statements)

References 18 publications

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“…Among all the signal pathways that regulate cancer development, the LncRNAs-miRNAs-mRNAs competing endogenous networks are closely associated with the pathogenesis of GC (3)(4)(5)(6), and this study, for the first time, identified a novel LncRNA MIR503HG/miR-224-5p/TUSC3 signaling cascade that regulated unfolded protein response (UPR) to hamper the development of GC. Specifically, we noticed that LncRNA MIR503HG was aberrantly downregulated in GC cells and tissues, and further experiments validated that LncRNA MIR503HG served as a tumor suppressor to reverse the malignant phenotypes and suppress aggressiveness of GC cells in vitro and in vivo, which are supported by the data from other teams in breast cancer (10), bladder cancer (11), and colorectal cancer (9). Then, the principles of ceRNA network mechanisms (12, 13) convince us to investigate the downstream targets of LncRNA MIR503HG, and we successfully screened out that LncRNA MIR503HG sponged miR-224-5p to upregulate TUSC3 in GC cells, and both miR-224-5p overexpression and TUSC3 ablation abrogated the tumor-inhibiting effects of LncRNA MIR503HG on GC, implying that LncRNA MIR503HG regulated the miR-224-5p/ TUSC3 axis to hamper GC progression.…”

Section: Discussionsupporting

confidence: 72%

“…Interestingly, their biological functions in regulating GC progression vary according to differential types of LncRNAs, and they act as both tumor suppressors (3,7,8) and oncogenes (4)(5)(6) in GC. Among all the LncRNAs, LncRNA MIR503 host gene (MIR503HG) is located on chromosome Xq26.3, and dysregulated LncRNA MIR503HG contributes to the aggressiveness of multiple cancers (9)(10)(11). Specifically, researcher notice that LncRNA MIR503HG functions as a tumor suppressor to restrain the development breast cancer (10), bladder cancer (11), and colorectal cancer (9), but it is still unclear whether LncRNA MIR503HG involves in regulating GC progression, thus, we selected LncRNA MIR503HG for further investigations in the present study.…”

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confidence: 99%

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The LncRNA MIR503HG/miR-224-5p/TUSC3 Signaling Cascade Suppresses Gastric Cancer Development via Modulating ATF6 Branch of Unfolded Protein Response

Lin

Wang

et al. 2021

Front. Oncol.

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BackgroundUnfolded protein response (UPR)-mediated tumor-promoting functions have been identified in multiple cancers, and this study focused on investigating the role and molecular mechanisms of UPR in modulating gastric cancer (GC) pathogenesis.MethodsThe bioinformatics analysis was performed to examine the expression status of cancer associated genes in patients with stomach adenocarcinoma (STAD) and predict the targeting sites of miR-224-5p with LncRNA MIR503HG and TUSC3. Genes expressions were quantified by Real-Time qPCR, Western Blot and immunohistochemistry (IHC). Cell proliferation, viability, apoptosis and mobility were evaluated by MTT assay, trypan blue staining assay, flow cytometer and transwell assay, respectively. The binding sites were validated by dual-luciferase reporter gene system assay.ResultsLncRNA MIR503HG and TUSC3 were downregulated, but miR-224-5p was upregulated in GC tissues and cells, in contrast with their normal counterparts. Further gain- and loss-of-function experiments validated that the malignant phenotypes in GC cells, including cell proliferation, invasion, epithelial-mesenchymal transition (EMT) and tumorigenesis, were negatively regulated by LncRNA MIR503HG. Mechanistically, LncRNA MIR503HG upregulated TUSC3 in GC cells through sponging miR-224-5p, resulting in the repression of GC progression. Finally, we validated that knock-down of ATF6, but not other two branches of UPR (PERK1 and IRE1), partially rescued cell proliferation and EMT in the GC cells with LncRNA MIR503HG overexpression.ConclusionsTargeting the LncRNA MIR503HG/miR-224-5p/TUSC3 signaling cascade suppressed ATF6-mediated UPR, resulting in the blockage of GC development.

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Section: Discussionsupporting

confidence: 72%

mentioning

confidence: 99%

The LncRNA MIR503HG/miR-224-5p/TUSC3 Signaling Cascade Suppresses Gastric Cancer Development via Modulating ATF6 Branch of Unfolded Protein Response

Lin

Wang

et al. 2021

Front. Oncol.

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“…Chuo et al . [32] demonstrated that MIR503HG overexpression inhibits colorectal cancer cell migration and invasion mediated by transforming growth factor‐β2. Lin et al .…”

Section: Discussionmentioning

confidence: 99%

Identification of crucial noncoding RNAs and mRNAs in hypertrophic scars via RNA sequencing

He²,

Zhang³

et al. 2021

FEBS Open Bio

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Hypertrophic scaring (HS) is a dermal fibroproliferative disorder characterized by excessive deposition of collagen and other extracellular matrix (ECM) components. The aim of this study is to explore crucial lncRNAs and circRNAs associated with HS and provide a better understanding of the molecular mechanism of HS. To investigate the lncRNA, circRNA and mRNA expression profiles we performed RNA-sequencing of human HS and normal skin tissues. Following the identification of differentially expressed mRNAs (DEmRNAs), lncRNAs (DElncRNAs) and circRNAs (DEcircRNAs), we performed functional enrichment of DEmRNAs. Further on, we constructed DElncRNA/DEcircRNA-DEmRNA co-expression networks and ceRNA regulatory networks and performed functional analyses of the DEmRNAs in the constructed networks. In total, 487 DEmRNAs, 92 DElncRNAs and 17 DEcircRNAs were identified. DEmRNAs were significantly enriched in processes like collagen fibril organization, ECM-receptor interaction and PI3K-Akt signaling pathway. Additionally, we detected 580 DElncRNA-DEmRNA and 505 DEcircRNA-DEmRNA co-expression pairs The ceRNA network contained 18 circRNA-miRNA pairs, 18 lncRNA-miRNA pairs and 409 miRNA-mRNA pairs, including 10 circRNAs, 5 lncRNAs, 15 miRNAs, and 160 mRNAs. We concluded that MIR503HG/hsa-miR-204-3p/ACAN, MIR503HG/hsa-miR-431-5p/TNFRSF9, MEG3/hsa-miR-6884-5p/ADAMTS14, AC000035.1-ADAMTS14 and hsa_circ_0069865-COMP/ADAM12 interaction pairs may play a central role in HS.

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“…For example, lncRNA LEF1-AS1 was found to accelerate CRC progression by targeting the miR-489/DIAPH1 axis (9). lncRNA MIR503HG alleviated CRC progression by downregulating TGF-β2 expression (10). In addition, lncRNA TTN-AS1 accelerated CRC progression by sponging miR-376a-3p (11).…”

Section: Introductionmentioning

confidence: 99%

lncRNA LUNAR1 accelerates colorectal cancer progression by targeting the miR‑495‑3p/MYCBP axis

Qian

Garg

et al. 2020

Int J Oncol

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Colorectal cancer (CRC) is a tumor type characterized by high patient morbidity and mortality. It has been reported that long non-coding (lncRNA) LUNAR1 (LUNAR1) participates in the regulation of tumor progression, such as diffuse large B-cell lymphoma. However, its role and underlying mechanisms in CRC progression have not been elucidated. The present study was designed to investigate the underlying mechanisms by which LUNAR1 regulates CRC progression. RT-qPCR and Pearson's correlation analysis revealed that LUNAR1 was highly expressed and was negatively associated with the overall survival of CRC patients. Moreover, CCK-8, clone formation, wound-healing migration, Transwell chamber and FACs assay analyses showed that LUNAR1 knockdown inhibited CRC cell proliferation, migration and invasion, while accelerating cell apoptosis. Additionally, LUNAR1 was found to function as a sponge of miR-495-3p, which was predicted by TargetScan and confirmed by luciferase reporter assay. Furthermore, functional studies indicated that miR-495-3p overexpression inhibited CRC cell proliferation, migration and invasion, while accelerating cell apoptosis. In addition, bioinformatics and luciferase reporter assays showed that miR-495-3p was found to negatively target Myc binding protein (MYCBP), and functional research showed that LUNAR1 accelerated CRC progression via the miR-495-3p/MYCBP axis. In conclusion, LUNAR1 accelerates CRC progression via the miR-495-3p/MYCBP axis, indicating that LUNAR1 may serve as a prognostic biomarker for CRC patients.

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LncRNA MIR503HG is downregulated in Han Chinese with colorectal cancer and inhibits cell migration and invasion mediated by TGF-β2

Cited by 19 publications

References 18 publications

The LncRNA MIR503HG/miR-224-5p/TUSC3 Signaling Cascade Suppresses Gastric Cancer Development via Modulating ATF6 Branch of Unfolded Protein Response

The LncRNA MIR503HG/miR-224-5p/TUSC3 Signaling Cascade Suppresses Gastric Cancer Development via Modulating ATF6 Branch of Unfolded Protein Response

Identification of crucial noncoding RNAs and mRNAs in hypertrophic scars via RNA sequencing

lncRNA LUNAR1 accelerates colorectal cancer progression by targeting the miR‑495‑3p/MYCBP axis

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