LncRNA MIR200CHG inhibits EMT in gastric cancer by stabilizing miR-200c from target-directed miRNA degradation

Abstract

Gastric cancer (GC) is a heterogeneous disease, threatening millions of lives worldwide, yet the functional roles of long non-coding RNAs (lncRNAs) in different GC subtypes remain poorly characterized. Microsatellite stable (MSS)/epithelial-mesenchymal transition (EMT) GC is the most aggressive subtype associated with a poor prognosis. Here, we apply integrated network analysis to uncover lncRNA heterogeneity between GC subtypes, and identify MIR200CHG as a master regulator mediating EMT specifically in MSS/EMT GC. The expression of MIR200CHG is silenced in MSS/EMT GC by promoter hypermethylation, associated with poor prognosis. MIR200CHG reverses the mesenchymal identity of GC cells in vitro and inhibits metastasis in vivo. Mechanistically, MIR200CHG not only facilitates the biogenesis of its intronic miRNAs miR-200c and miR-141, but also protects miR-200c from target-directed miRNA degradation (TDMD) through direct binding to miR-200c. Our studies reveal a landscape of a subtype-specific lncRNA regulatory network, providing clinically relevant biological insights towards MSS/EMT GC.

Proteome Analysis Related to Unsaturated Fatty Acid Synthesis by Interfering with Bovine Adipocyte ACSL1 Gene

“…However, it is noteworthy that the protein levels and mRNA expression do not always exhibit a direct correlation [ 31 , 32 ], with proteins also providing valuable insights into post-translational modifications [ 33 , 34 ]. Additionally, lncRNAs regulate gene expression at the transcriptional and post-transcriptional levels [ 35 , 36 ], but they also regulate the transcription of downstream molecules by binding to proteins and localizing protein complexes to specific DNA sequences [ 37 , 38 ]. In this study, label-free proteomics analysis was performed using RNA interference and non-interference with ACSL1 in bovine adipocytes ( Figure 1 ).…”