LncRNA LINC00467 acted as an oncogene in esophageal squamous cell carcinoma by accelerating cell proliferation and preventing cell apoptosis via the miR‐485‐5p/DPAGT1 axis

Abstract

Background and Aim Esophageal carcinoma has been regarded as one of the top 10 common malignancies globally. Esophageal squamous cell carcinoma (ESCC) is an important subtype of esophageal carcinoma with approximately 20% survival rate. Long noncoding RNAs were documented to regulate the occurrence or progression of several tumors. However, neither the biological role nor the molecular mechanism of LINC00467 has been explored. This research is aimed to investigating the regulatory mechanism of LINC00467 in ESCC. Methods In this study, a series of experiments including reverse transcription–quantitative polymerase chain reaction, Cell Counting Kit‐8, luciferase reporter, western blot, and RNA immunoprecipitation were designed and conducted to explore the potential function and mechanism of LINC00467 in ESCC. Results According to experimental results, we found out upregulated LINC00467 improved cell proliferation, but hindered cell apoptosis. In mechanism, miR‐485‐5p was predicted, screened out, and validated to combine with LINC00467, which displayed lower expression in ESCC. Additionally, miR‐485‐5p negatively regulated and directly targeted DPAGT1. Rescue assays suggested that DPAGT1 amplification was able to recover the influence of LINC00467 deficiency on cell proliferation and apoptosis. Furthermore, knockdown of LINC00467 suppressed tumor growth in vivo. Conclusion We proved that LINC00467 acted as an oncogene in ESCC by accelerating cell proliferation and preventing cell apoptosis via miR‐485‐5p/DPAGT1 axis. This may provide a potential diagnostic marker for ESCC treatment.

Integrated analysis of high-throughput sequencing data reveals the key role of LINC00467 in the invasion and metastasis of testicular germ cell tumors

“…It has been shown that LINC00467 promotes cell proliferation and migration in liver cancer by binding to IGF2BP3 to enhance the stability of its cis-target genes [22]. In esophageal cancer, LINC00467 can promote esophageal cancer cell proliferation by adsorbing miR-485-5p and reducing the inhibitory effect on the miRNA target gene, DPAGT1 [23]. Previously, our group found that LINC00467 can regulate the phosphorylation of AKT in non-small cell lung cancer by binding to AZGP1 to promote cancer cell migration and invasion [8].…”

Linc00467 promotion of gastric cancer development by directly regulating miR-7-5p expression and downstream epidermal growth factor receptor

“…Further analysis of the author indicated that linc00467 can directly regulate the miR-9-5a expression and downstream target peroxisome proliferator activated receptor alpha signaling [ 18 ]. Additionally, a study regarding esophageal carcinoma also found that upregulated linc00467 improved cell proliferation while inhibiting cell apoptosis by regulating the axis of miR-485-5p/dolichyl-phosphate N-acetylglucosaminephosphotransferase 1 [ 27 ]. Research regarding linc00467 outside the digestive system in malignant tumors mainly focused on lung cancer and glioma.…”

LINC00162 regulates cell proliferation and apoptosis by sponging PAQR4‐targeting miR‐485‐5p

“…Our findings reveal that LINC00162 functions as a sponge of miR‐485‐5. Several lncRNAs are reported to function as sponge of miR‐485‐5p (Bao et al, 2019; Liu, Yang, et al, 2020; Tu et al, 2019; Wang et al, 2018; Zhang, Hu, et al, 2018). In hepatocellular cell carcinoma, lncRNA DSCR8 activates the Wnt/β‐catenin pathway by decoying FZD7 ‐targeting miR‐485‐5p (Wang et al, 2018).…”