LncRNA HOXA‐AS2 positively regulates osteogenesis of mesenchymal stem cells through inactivating NF‐κB signalling

Zhu, Xinxing; Yu, Jinjin; Du, Jiang; Zhong, Genshen; Qiao, Liang; Lin, Juntang

doi:10.1111/jcmm.14034

Cited by 14 publications

(17 citation statements)

References 31 publications

(66 reference statements)

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“…Discard the supernatant, rinse the precipitates with DMEM and then centrifuge at 250 g for 5 minutes. The detailed protocol for UCMSCs isolation and culture was performed as previously reported …”

Section: Methodsmentioning

confidence: 99%

“…For Alizarin Red staining, MenSCs were first fixed in 70% ethanol, followed by 1% Alizarin Red solution staining for 1 minute. The detailed protocol was performed as previously described . For the detection of ALP activity, cells were first fixed with 70% ethanol for 30 minutes and then subjected to the BCIP/NBT liquid substrate (0.1 mol/L 2‐amino‐2‐methyl‐1‐propanol, 1 mmol/L MgCl2 and 8 mmol/L P‐nitrophenyl phosphate disodium) incubation at 37°C for 30 minutes.…”

Section: Methodsmentioning

confidence: 99%

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LncRNA NKILA integrates RXFP1/AKT and NF‐κB signalling to regulate osteogenesis of mesenchymal stem cells

Zhang

Cao

et al. 2019

J Cellular Molecular Medi

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Mesenchymal stem cells (MSCs) are previously found to have potential capacity to differentiate into osteocytes when exposed to specific stimuli. However, the detailed molecular mechanism during this progress remains largely unknown. In the current study, we characterized the lncRNA NKILA as a crucial positive regulator for osteogenesis of MSCs. NKILA attenuation significantly inhibits the calcium deposition and alkaline phosphatase activity of MSCs. More interestingly, we defined that NKILA is functionally involved in the regulation of RXFP1/PI3K‐AKT and NF‐κB signalling. Knockdown of NKILA dramatically down‐regulates the expression of RXFP1 and then reduces the activity of AKT, a downstream regulator of RXFP1 signalling which is widely accepted as an activator of osteogenesis. Moreover, we identify NF‐κB as another critical regulator implicated in NKILA‐mediated osteogenic differentiation. Inhibition of NF‐κB can induce the expression of RUNX2, a master transcription factor of osteogenesis, in a HDAC2‐mediated deacetylation manner. Thus, this study illustrates the regulatory function of NKILA in osteogenesis through distinct signalling pathways, therefore providing a new insight into searching for new molecular targets for bone tissue repair and regeneration.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

LncRNA NKILA integrates RXFP1/AKT and NF‐κB signalling to regulate osteogenesis of mesenchymal stem cells

Zhang

Cao

et al. 2019

J Cellular Molecular Medi

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“…Equal volume PBS was added to the samples and then subjected to standard Ficoll procedures. Subsequently, the detailed steps for MenSCs isolation and culture were conducted as previously described . For UCMSC isolation and culture, the umbilical cord vessels were first subjected to disinfection in 75% ethanol for 1 minute and then were cut into cubes.…”

Section: Methodsmentioning

confidence: 99%

“…Subsequently, the supernatant was discarded and the precipitates were rinsed with DMEM. In the following, the detailed protocol for UCMSC isolation and culture was conducted as previously reported …”

Section: Methodsmentioning

confidence: 99%

“…For Alizarin Red staining, MenSCs were first fixed in 70% ethanol and then subjected to 1% Alizarin Red solution staining for 1 minute. The detailed procedures were performed as previously described . For the detection of ALP activity, cells were first fixed with 70% ethanol for 30 minutes and then incubated with the BCIP/NBT liquid substrate at 37°C for 30 minutes.…”

Section: Methodsmentioning

confidence: 99%

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LncRNA HOTAIRM1 promotes osteogenesis by controlling JNK/AP‐1 signalling‐mediated RUNX2 expression

Peng

et al. 2019

J Cell Mol Med

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Mesenchymal stem cells (MSCs) have potential ability to differentiate into osteocytes in response to in vitro specific induction. However, the molecular basis underlying this biological process remains largely unclear. In this study, we identify lncRNA HOTAIRM1 as a critical regulator to promote osteogenesis of MSCs. Loss of HOTAIRM1 significantly inhibits the calcium deposition and alkaline phosphatase activity of MSCs. Mechanistically, we find that HOTAIRM1 positively modulates the activity of JNK and c‐Jun, both of which are widely accepted as crucial regulators of osteogenic differentiation. More importantly, c‐Jun is found to be functionally involved in the regulation of RUNX2 expression, a master transcription factor of osteogenesis. In detail, c‐Jun can help recruit the acetyltransferase p300 to RUNX2 promoter, facilitating acetylation of histone 3 at K27 site, therefore epigenetically activating RUNX2 gene transcription. In summary, this study highlights the functional importance of HOTAIRM1 in regulation of osteogenesis, and we characterize HOTAIRM1 as a promising molecular target for bone tissue repair and regeneration.

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LncRNA HOXA‐AS2 positively regulates osteogenesis of mesenchymal stem cells through inactivating NF‐κB signalling

Cited by 14 publications

References 31 publications

LncRNA NKILA integrates RXFP1/AKT and NF‐κB signalling to regulate osteogenesis of mesenchymal stem cells

LncRNA NKILA integrates RXFP1/AKT and NF‐κB signalling to regulate osteogenesis of mesenchymal stem cells

LncRNA HOTAIRM1 promotes osteogenesis by controlling JNK/AP‐1 signalling‐mediated RUNX2 expression

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