LncRNA HOXA‐AS2 positively regulates osteogenesis of mesenchymal stem cells through inactivating NF‐κB signalling

Zhu, Xinxing; Yu, Jinjin; Du, Jinyan; Zhong, Genshen; Qiao, Liang; Lin, Juntang

doi:10.1111/jcmm.14034

Cited by 16 publications

(17 citation statements)

References 31 publications

(66 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Chen et al reported that overexpression of lncRNA XIST led to the inhibition of osteogenic differentiation of BMSCs in 3-week-old Sprague Dawley rats [156], thus, its inhibition through specific inhibitor can revert the phenomenon and can treat the senile osteoporosis. Most recently, Zhu et al have identified lncRNA HOXA-AS2 as a key positive regulator in causing osteogenesis in BMSCs through NF-κB signaling inactivation [157], which may act as a new therapeutic target against senile osteoporosis.…”

Section: Treatment Of Senile Osteoporosis By Aiming At Bmscsmentioning

confidence: 99%

Senile Osteoporosis: The Involvement of Differentiation and Senescence of Bone Marrow Stromal Cells

Qadir

Liang

et al. 2020

IJMS

120

View full text Add to dashboard Cite

Senile osteoporosis has become a worldwide bone disease with the aging of the world population. It increases the risk of bone fracture and seriously affects human health. Unlike postmenopausal osteoporosis which is linked to menopause in women, senile osteoporosis is due to aging, hence, affecting both men and women. It is commonly found in people with more than their 70s. Evidence has shown that with age increase, bone marrow stromal cells (BMSCs) differentiate into more adipocytes rather than osteoblasts and undergo senescence, which leads to decreased bone formation and contributes to senile osteoporosis. Therefore, it is necessary to uncover the molecular mechanisms underlying the functional changes of BMSCs. It will benefit not only for understanding the senile osteoporosis development, but also for finding new therapies to treat senile osteoporosis. Here, we review the recent advances of the functional alterations of BMSCs and the related mechanisms during senile osteoporosis development. Moreover, the treatment of senile osteoporosis by aiming at BMSCs is introduced.

show abstract

Section: Treatment Of Senile Osteoporosis By Aiming At Bmscsmentioning

confidence: 99%

Senile Osteoporosis: The Involvement of Differentiation and Senescence of Bone Marrow Stromal Cells

Qadir

Liang

et al. 2020

IJMS

120

View full text Add to dashboard Cite

show abstract

“…For Alizarin Red staining, MenSCs were first fixed in 70% ethanol and then subjected to 1% Alizarin Red solution staining for 1 minute. The detailed procedures were performed as previously described . For the detection of ALP activity, cells were first fixed with 70% ethanol for 30 minutes and then incubated with the BCIP/NBT liquid substrate at 37°C for 30 minutes.…”

Section: Methodsmentioning

confidence: 99%

LncRNA HOTAIRM1 promotes osteogenesis by controlling JNK/AP‐1 signalling‐mediated RUNX2 expression

Peng

et al. 2019

J Cellular Molecular Medi

View full text Add to dashboard Cite

Mesenchymal stem cells (MSCs) have potential ability to differentiate into osteocytes in response to in vitro specific induction. However, the molecular basis underlying this biological process remains largely unclear. In this study, we identify lncRNA HOTAIRM1 as a critical regulator to promote osteogenesis of MSCs. Loss of HOTAIRM1 significantly inhibits the calcium deposition and alkaline phosphatase activity of MSCs. Mechanistically, we find that HOTAIRM1 positively modulates the activity of JNK and c‐Jun, both of which are widely accepted as crucial regulators of osteogenic differentiation. More importantly, c‐Jun is found to be functionally involved in the regulation of RUNX2 expression, a master transcription factor of osteogenesis. In detail, c‐Jun can help recruit the acetyltransferase p300 to RUNX2 promoter, facilitating acetylation of histone 3 at K27 site, therefore epigenetically activating RUNX2 gene transcription. In summary, this study highlights the functional importance of HOTAIRM1 in regulation of osteogenesis, and we characterize HOTAIRM1 as a promising molecular target for bone tissue repair and regeneration.

show abstract

“…Discard the supernatant, rinse the precipitates with DMEM and then centrifuge at 250 g for 5 minutes. The detailed protocol for UCMSCs isolation and culture was performed as previously reported …”

Section: Methodsmentioning

confidence: 99%

“…For Alizarin Red staining, MenSCs were first fixed in 70% ethanol, followed by 1% Alizarin Red solution staining for 1 minute. The detailed protocol was performed as previously described . For the detection of ALP activity, cells were first fixed with 70% ethanol for 30 minutes and then subjected to the BCIP/NBT liquid substrate (0.1 mol/L 2‐amino‐2‐methyl‐1‐propanol, 1 mmol/L MgCl2 and 8 mmol/L P‐nitrophenyl phosphate disodium) incubation at 37°C for 30 minutes.…”

Section: Methodsmentioning

confidence: 99%

“…Subsequently, the detailed steps for MenSCs isolation and culture were conducted as previously described. 25 For UCMSCs isolation and culture, the umbilical cord vessels were first subjected to disinfection in 75% ethanol for 1 minute and then cut it into cubes. Discard the supernatant, rinse the precipitates with DMEM and then centrifuge at 250 g for 5 minutes.…”

Section: Isolation and Culture Of Menscs And Ucmscsmentioning

confidence: 99%

See 1 more Smart Citation

LncRNA NKILA integrates RXFP1/AKT and NF‐κB signalling to regulate osteogenesis of mesenchymal stem cells

Zhang

Cao

et al. 2019

J Cellular Molecular Medi

View full text Add to dashboard Cite

Mesenchymal stem cells (MSCs) are previously found to have potential capacity to differentiate into osteocytes when exposed to specific stimuli. However, the detailed molecular mechanism during this progress remains largely unknown. In the current study, we characterized the lncRNA NKILA as a crucial positive regulator for osteogenesis of MSCs. NKILA attenuation significantly inhibits the calcium deposition and alkaline phosphatase activity of MSCs. More interestingly, we defined that NKILA is functionally involved in the regulation of RXFP1/PI3K‐AKT and NF‐κB signalling. Knockdown of NKILA dramatically down‐regulates the expression of RXFP1 and then reduces the activity of AKT, a downstream regulator of RXFP1 signalling which is widely accepted as an activator of osteogenesis. Moreover, we identify NF‐κB as another critical regulator implicated in NKILA‐mediated osteogenic differentiation. Inhibition of NF‐κB can induce the expression of RUNX2, a master transcription factor of osteogenesis, in a HDAC2‐mediated deacetylation manner. Thus, this study illustrates the regulatory function of NKILA in osteogenesis through distinct signalling pathways, therefore providing a new insight into searching for new molecular targets for bone tissue repair and regeneration.

show abstract

LncRNA HOXA‐AS2 positively regulates osteogenesis of mesenchymal stem cells through inactivating NF‐κB signalling

Cited by 16 publications

References 31 publications

Senile Osteoporosis: The Involvement of Differentiation and Senescence of Bone Marrow Stromal Cells

Senile Osteoporosis: The Involvement of Differentiation and Senescence of Bone Marrow Stromal Cells

LncRNA HOTAIRM1 promotes osteogenesis by controlling JNK/AP‐1 signalling‐mediated RUNX2 expression

LncRNA NKILA integrates RXFP1/AKT and NF‐κB signalling to regulate osteogenesis of mesenchymal stem cells

Contact Info

Product

Resources

About