LncRNA FLVCR1‐AS1 acts as miR‐513c sponge to modulate cancer cell proliferation, migration, and invasion in hepatocellular carcinoma

Abstract

In the present study, we aimed to search for dysregulated lnRNAs in Hepatocellular carcinoma (HCC) tissues, and analyze the relationship of its expression level with the clinicopathological feature and patient prognosis. The biological function of FLVCR1-AS1, the identified lncRNA, in the process of HCC development, and progression was investigated in vitro and in vivo. The underlying molecular mechanism was further explored. We determined FLVCR1-AS1 expression in HCC tissues and peri-tumor tissues by bioinformatic analysis, qRT-PCR, Northern blot and in situ hybridization. The relationship between FLVCR1-AS1 expression level and prognosis was determined by analyzing clinical samples. The effects of FLVCR1-AS1 knockdown on HCC cell proliferation, apoptosis, migration, and invasion were investigated by CCK8, FACS, and tanswell assay, respectively. Tumor xenograft model was used to determine the influence of down-regulated FLVCR1-AS1 on tumor growth and metastasis. lncRNA FLVCR1-AS1 was extremely up-regulated in HCC tissues and cell lines. FLVCR1-AS1 expression level was positively correlated with tumor severity. FLVCR1-AS1 knockdown remarkably inhibited HCC cell proliferation, migration, and invasion in vitro and in vivo while induced cell apoptosis. In mechanism, FLVCR1-AS1 acted as a competitive endogenous RNAs to sponge miR-513c which targeted the mRNA of MET for degradation. By directly sponging miR-513c, FLVCR1-AS1 increased MET expression in HCC, and then promoted HCC progression. It was demonstrated that FLVCR1-AS1 played a positive role in HCC development and progression according to the study in its mechanism, function and clinical manifestation, so that it could be expected to become a new target in HCC prevention and treatment.

LncRNA FLVCR1-AS1 mediates miR-513/YAP1 signaling to promote cell progression, migration, invasion and EMT process in ovarian cancer

“…FLVCR1-AS1 expression has been reported to be upregulated in lung gastric and hepatocellular cancers [11][12][13][14]. Similarly, elevated FLVCR1-AS1 expressions were found in OSC tissues, serums, and cell lines in the current study.…”

“…FLVCR1-AS1 is recently discovered upregulated in hepatocellular cancer, gastric cancer and lung cancer [11][12][13][14]. However, few studies have studied FLVCR1-AS1 in OSC.…”

Assessment of microRNA‐144‐5p and its putative targets in inflamed gingiva from chronic periodontitis patients

“…In addition, miR‐204 may also be involved in the chondrogenic differentiation of periodontal ligament stem cells . miR‐513c was reported to inhibit the development of nervous system tumours and hepatocellular carcinoma . Nevertheless, little research has been conducted to reveal their roles in periodontal inflammation.…”

“…64 miR-513c was reported to inhibit the development of nervous system tumours and hepatocellular carcinoma. [65][66][67] Nevertheless, little research has been conducted to reveal their roles in periodontal inflammation.…”

LINC00857 contributes to hepatocellular carcinoma malignancy via enhancing epithelial‐mesenchymal transition

“…LncRNA TUG1 contributes to the HCC development through interacting with microRNA (miR)‐144 . In addition, lncRNA FLVCR1‐AS1 modulates HCC cell progression by sponging miR‐513c . Recently, LINC00857 has been reported to play a crucial role in various cancers.…”