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Mishima, Masaki; Ozaki, Jun; Kabe, Yoshio; Goto, Masahide; Ueda, Hitoshi; Hirose, Susumu; Handa, Hiroshi; Shirakawa, Masahiro

doi:10.1023/a:1008347729176

Cited by 11 publications

(5 citation statements)

References 9 publications

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“…Since then, it has been investigated in several other organisms and shown to bridge different transcription factors, containing sequence-specific DNA-binding domains, to TBP, but not to bind directly to DNA (reviewed in de Koning et al, 2009 ), suggesting it functions by recruiting TBP to promoters where transcription factors are found (Ozaki et al, 1999 ). Structural analyses were able to precise that the C-terminal end corresponds to a well-structured helix-turn-helix (HTH) motif (Mishima et al, 1999 ; Ozaki et al, 1999 ).…”

Section: Introductionmentioning

confidence: 99%

Function Analysis of MBF1, a Factor Involved in the Response to Amino Acid Starvation and Virulence in Candida albicans

Amorim-Vaz

Coste

Tran

et al. 2021

Front. Fungal Biol.

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Candida albicans is a commensal of human mucosae, but also one of the most common fungal pathogens of humans. Systemic infections caused by this fungus, mostly affecting immunocompromised patients, are associated to fatality rates as high as 50% despite the available treatments. In order to improve this situation, it is necessary to fully understand how C. albicans is able to cause disease and how it copes with the host defenses. Our previous studies have revealed the importance of the C. albicans gene MBF1 in virulence and ability to colonize internal organs of mammalian and insect hosts. MBF1 encodes a putative transcriptional regulator, and as such it likely has an impact in the regulation of C. albicans gene expression during host infection. Here, recent advances in RNA-seq technologies were used to obtain a detailed analysis of the impact of MBF1 on C. albicans gene expression both in vitro and during infection. MBF1 was involved in the regulation of several genes with a role in glycolysis and response to stress, particularly to nutritional stress. We also investigated whether an interaction existed between MBF1 and GCN4, a master regulator of response to starvation, and found that both genes were needed for resistance to amino acid starvation, suggesting some level of interaction between the two. Reinforcing this idea, we showed that the proteins encoded by both genes could interact. Consistent with the role of MBF1 in virulence, we also established that GCN4 was necessary for virulence in the mouse model of systemic infection as well as in the Galleria mellonella infection model.

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Section: Introductionmentioning

confidence: 99%

Function Analysis of MBF1, a Factor Involved in the Response to Amino Acid Starvation and Virulence in Candida albicans

Amorim-Vaz

Coste

Tran

et al. 2021

Front. Fungal Biol.

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“…The N- and C-termini sequences are divergent among different organisms, whereas the HTH domain, which contains four α-helices, is conserved 2 . The HTH domain is responsible for the functional activity of MBF1, and differences in the N- and C-termini do not affect the main activities of MBF1 proteins 3 , 4 .…”

Section: Introductionmentioning

confidence: 99%

Modification and functional adaptation of the MBF1 gene family in the lichenized fungus Endocarpon pusillum under environmental stress

Wang

Wei

Huang

et al. 2017

Sci Rep

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The multiprotein-bridging factor 1 (MBF1) gene family is well known in archaea, non-lichenized fungi, plants, and animals, and contains stress tolerance-related genes. Here, we identified four unique mbf1 genes in the lichenized fungi Endocarpon spp. A phylogenetic analysis based on protein sequences showed the translated MBF1 proteins of the newly isolated mbf1 genes formed a monophyletic clade different from other lichen-forming fungi and Ascomycota groups in general, which may reflect the evolution of the biological functions of MBF1s. In contrast to the lack of function reported in yeast, we determined that lysine114 in the deduced Endocarpon pusillum MBF1 protein (EpMBF1) had a specific function that was triggered by environmental stress. Further, the Endocarpon-specific C-terminus of EpMBF1 was found to participate in stress tolerance. Epmbf1 was induced by a number of abiotic stresses in E. pusillum and transgenic yeast, and its stress-resistant ability was stronger than that of the yeast mbf1. These findings highlight the evolution and function of EpMBF1 and provide new insights into the co-evolution hypothesis of MBF1 and TATA-box-binding proteins.

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“…In addition to the MBF1 domain (PF08523), the MBF1 protein includes an HTH XRE-family domain (SM000530), also known as a Cro/C1-type HTH domain (IPR001387), located in the C-terminus, that is annotated as a DNA-binding domain of transcriptional regulators. The HTH XRE domain contains four α-helices, and is responsible for MBF1 function [25–27]. In the present study, purified recombinant rPoxMBF1 protein could directly bind to the promoter regions of major cellulase and xylanase genes in vitro, thereby controlling their transcriptional levels in P .…”

Section: Discussionmentioning

confidence: 80%

Differential transcriptomic profiling of filamentous fungus during solid-state and submerged fermentation and identification of an essential regulatory gene PoxMBF1 that directly regulated cellulase and xylanase gene expression

Zhao

Liu

Wang

et al. 2019

Biotechnol Biofuels

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Background Solid-state fermentation (SSF) mimics the natural decay environment of soil fungi and can be employed to investigate the production of plant biomass-degrading enzymes. However, knowledge on the transcriptional regulation of fungal genes during SSF remains limited. Herein, transcriptional profiling was performed on the filamentous fungus Penicillium oxalicum strain HP7-1 cultivated in medium containing wheat bran plus rice straw (WR) under SSF (WR_SSF) and submerged fermentation (WR_SmF; control) conditions. Novel key transcription factors (TFs) regulating fungal cellulase and xylanase gene expression during SSF were identified via comparative transcriptomic and genetic analyses. Results Expression of major cellulase genes was higher under WR_SSF condition than that under WR_SmF, but the expression of genes involved in the citric acid cycle was repressed under WR_SSF condition. Fifty-six candidate regulatory genes for cellulase production were screened out from transcriptomic profiling of P . oxalicum HP7-1 for knockout experiments in the parental strain ∆ PoxKu70 , resulting in 43 deletion mutants including 18 constructed in the previous studies. Enzyme activity assays revealed 14 novel regulatory genes involved in cellulase production in P . oxalicum during SSF. Remarkably, deletion of the essential regulatory gene PoxMBF1 , encoding Multiprotein Bridging Factor 1, resulted in doubled cellulase and xylanase production at 2 days after induction during both SSF and SmF. PoxMBF1 dynamically and differentially regulated transcription of a subset of cellulase and xylanase genes during SSF and SmF, and conferred stress resistance. Importantly, PoxMBF1 bound specifically to the putative promoters of major cellulase and xylanase genes in vitro. Conclusions We revealed differential transcriptional regulation of P . oxalicum during SSF and SmF, and identified PoxMBF1, a novel TF that directly regulates cellulase and xylanase gene expression during SSF and SmF. These findings expand our understanding of regulatory mechanisms of cellulase and xylanase gene expression during fungal fermentation. Electronic supplementary material The online version of this article (10.1186/s13068-019-1445-4) contains supplementary material, which is available to authorized users.

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Cited by 11 publications

References 9 publications

Function Analysis of MBF1, a Factor Involved in the Response to Amino Acid Starvation and Virulence in Candida albicans

Function Analysis of MBF1, a Factor Involved in the Response to Amino Acid Starvation and Virulence in Candida albicans

Modification and functional adaptation of the MBF1 gene family in the lichenized fungus Endocarpon pusillum under environmental stress

Differential transcriptomic profiling of filamentous fungus during solid-state and submerged fermentation and identification of an essential regulatory gene PoxMBF1 that directly regulated cellulase and xylanase gene expression

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