Live imaging of newly formed lymphatic vessels in the cornea

Angiographic and In Vivo Confocal Microscopic Characterization of Human Corneal Blood and Presumed Lymphatic Neovascularization

“…27 Although the evidence is not clear, the hyperfluorescence adjacent, and in some cases separate from larger venules, could be interpreted as laminar flow. Similar to that described by Yeun et al, 26 we were able to image colocalized corneal vessels by IVCM, some of which carried nucleated cells. Although these properties have previously been used to identify neovascular lymphatic vessels in murine and human corneas, 31-33 we were not able to determine whether they were lymphatic vessels.…”

“…It has previously been shown in the mouse model that corneal neovascular lymphatic vessels can be colocalized to blood vessels, being of a smaller diameter than the neighboring red blood vessels. 26 We were able to confirm such neighboring small-diameter possible lymphatic vessels by IVCM in this study. 27 In case of colocalization of corneal blood and lymphatic vessels, DSA would not be expected to show any newly apparent vessels, except in nonoverlapping areas along the border of a blood vessel.…”

Corneal Lymphatic Valve Formation in Relation to Lymphangiogenesis

“…22,23 The dye drainage was imaged with a customized imaging system including an adjustable eye and head stage holder and the Axio Zoom.V16 microscope (Carl Zeiss AG).…”

Watching lymphatic vessels grow by making them glow

“…As shown by the current study of Yuen and colleagues [4], the easily accessible location and transparent nature of the cornea make it ideal for in vivo fluorescent imaging. The study describes novel techniques of video-rate stereomicroscopy and high resolution 2-photon microscopy to image the growth of the lymphatic vessels in the cornea after inflammation was induced by suture placement [4].…”

“…The study describes novel techniques of video-rate stereomicroscopy and high resolution 2-photon microscopy to image the growth of the lymphatic vessels in the cornea after inflammation was induced by suture placement [4]. With injection of fluorescent dextran particles into the subconjunctival space, the normally invisible lymphatic vessels could be visualized.…”