Lithium Tantalate Surface Acoustic Wave Sensors for Bio-Analytical Applications

Abstract

A novel chip-based measurement system for marker-free detection of afrinity and molecular interaction kinetics has been developed. Lithium tantalate (LiTaO3) based Love-wave sensors are utilized as an alternative to quartz as substrate material in order to improve both the sensitivity and the limit-of-detection (LOD). Monitoring the coupling of thrombin to its antibody at low concentrations 1 nM and 10 nM showed phase changes of A(p = 0.100 and 0.350, respectively, for an active sensor area of 5.6 mm2. We have found that the estimated detection limit for thrombin is on the order of 0.18 nM for our LiTaO3 sensors.I.

Epitope structure and binding affinity of single chain llama anti‐β‐amyloid antibodies revealed by proteolytic excision affinity‐mass spectrometry

“…A surface acoustic wave (SAW) biosensor was employed for bioaffinity detection, as an alternative to Surface Plasmon Resonance, that provides bioaffinity quantification by determination of mass and viscosity because of the biomacromolecular interactions (Malave et al ., ; Perpeet et al ., ; Gronewold, ; Dragusanu et al ., ). Determinations of affinity constants (K D ) were performed for Aβ(17–28) in comparison with Aβ(1–40) after immobilization of the peptides to activated Au‐SAM using different concentrations of Nb_3 and Nb_9, as described in Materials and Methods.…”

Antigen-antibody selective recognition using LiTaO3SH-SAW sensors: investigations on macromolecules effects on binding kinetic constants

“…Surface acoustic waves sensors (SAW sensors), whose principle is based on variations of propagation media properties, were largely used for gas detection [1,2], and have recently revealed their great potential for chemical and biological species detection in liquid media [3][4][5][6][7][8][9]. These devices are still subjected to many technological developments, offering thus new prospects for label free detection of increasingly small quantities in real time.…”

Sputter Deposited Magnetostrictive Layers for SAW Magnetic Field Sensors

“…The use of different piezoelectric substrates and crystallographic orientations allows the excitation of various types of surface acoustic waves, like seismic waves such as Rayleigh waves or shear horizontal waves called Love waves. Depending on the different surface waveforms, sensors can be used for different measurement applications such as biological molecule detection [2,3] and temperature [4]. A large area of application for SAW sensors is a magnetic field sensor [4][5][6][7][8] and a tabular comparison can be found in the Appendix A (see Table A1).…”

“…The sensors are based on various substrates and magnetic sensitive layers. Typical substrates are St-cut Quartz in different cutting directions, LiNbO 3 and LiTaO 3 [2][3][4][5][6][7][8][9][10][11][12]. In that context, a wide range of ferromagnetic materials are used for the magnetic sensitive layer, e.g., FeCo, FeGa, Fe 2 Tb and (Fe 90 Co 10 ) 78 Si 12 B 10 (FeCoSiB) [10][11][12], which are deposited on the SAW sensor as full films, multilayers or patterned structures [12][13][14].…”