Literature Review Concerning Cell and Skin Substitute Cultures Obtained by Means of Tissue Engineering used in the Treatment of Burns

Cichowski, Andrzej; Kawecki, Marek; Glik, Justyna; Klama-Baryła, Agnieszka; Łabuś, Wojciech; Maj, Mariusz; Nowak, Marcin; Dworak, Andrzej; Sieroń, Aleksander

doi:10.2478/pjs-2014-0037

Cited by 7 publications

(4 citation statements)

References 40 publications

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“…Since mature cells are mostly terminally differentiated, these cells are not the first choice for tissue engineering and regenerative medicine compared to stem cells [168]. For more information concerning several skin tissue engineering methods, the authors would like to refer to other review papers [170,171,172]. …”

Section: Regenerative Therapies For Skin Wound Managementmentioning

confidence: 99%

Regenerative Skin Wound Healing in Mammals: State-of-the-Art on Growth Factor and Stem Cell Based Treatments

Borena

Martens

Broeckx

et al. 2015

Cell Physiol Biochem

168

112

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Mammal skin has a crucial function in several life-preserving processes such as hydration, protection against chemicals and pathogens, initialization of vitamin D synthesis, excretion and heat regulation. Severe damage of the skin may therefore be life-threatening. Skin wound repair is a multiphased, yet well-orchestrated process including the interaction of various cell types, growth factors and cytokines aiming at closure of the skin and preferably resulting in tissue repair. Regardless various therapeutic modalities targeting at enhancing wound healing, the development of novel approaches for this pathology remains a clinical challenge. The time-consuming conservative wound management is mainly restricted to wound repair rather than restitution of the tissue integrity (the so-called “restitutio ad integrum”). Therefore, there is a continued search towards more efficacious wound therapies to reduce health care burden, provide patients with long-term relief and ultimately scarless wound healing. Recent in vivo and in vitro studies on the use of skin wound regenerative therapies provide encouraging results, but more protracted studies will have to determine whether the effect of observed effects are clinically significant and whether regeneration rather than repair can be achieved. For all the aforementioned reasons, this article reviews the emerging field of regenerative skin wound healing in mammals with particular emphasis on growth factor- and stem cell-based therapies.

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Section: Regenerative Therapies For Skin Wound Managementmentioning

confidence: 99%

Regenerative Skin Wound Healing in Mammals: State-of-the-Art on Growth Factor and Stem Cell Based Treatments

Borena

Martens

Broeckx

et al. 2015

Cell Physiol Biochem

168

112

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“…[9,25~27] . Hyalograft 3D 是以自体成纤维细胞和同种异体支架材料-透明质酸膜在体外三维培养构建的人工真皮 [9] , 治疗深度烧伤具有显著效果, 能有效促进创面上皮化, 抑制瘢痕增生和创面收缩 [28] . [27] .…”

Section: 由种子细胞和支架材料体外三维构建培养的组织工程皮肤unclassified

“…2007 年, 中国的组织工程皮肤技术也发展成熟, 国内相关产品完成注册. 至今, 已有各种类型组织工程皮肤产品面市, 如 Apligraf、安体肤、Epicel, EpiDex, LaserSkin, Bioseed-S, TransCyte, Dermagraft, Hyalograft 3D, Cell-Spray, Alloderm, GraftJacket, OASIS, E Z Derm, Integra 和 Biobrane 等, 还有十几种皮肤替代物产品处于在研阶段 [9] , 组织工程皮肤发展史见图 1.…”

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The Development of Tissue Engineering Skin

Yang¹,

Cui

2015

Sci. Sin.-Vitae

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Tissue engineering skin (TE Skin) technology has been developed rapidly from the beginning to now. In this review, products of TE Skin are classified into 3 types: TE Skin composed of cells and scaffold, TE Skin from cells only, TE Skin form with several scaffold materials. Each type of TE Skin was divided into 2 or 3 categories according to structure, morphology and origin, and representative products of every category were given specific description. Insufficent capacity of regeneration and repairing, limited cell sources, the high cost of maufacture and transportation, discussion and analysis of existing problems in TE Skin are conducted. Management of TE Skin is also discussed, and some extended applications of TE Skin are proposed, such as cosmetic test kit. Through summing up technical achievements, discussion of research focuses and analysis of future prospects, we hope this review to help understand the field TE Skin.

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“…Current treatment methods for facial skin burn injuries mainly include the use of autologous skin grafts and alternative skin substitutes. Autologous skin grafts are the gold standard of care for skin burns and any full-thickness loss of skin integrity; however, it has been significantly limited by donor site availability and morbidity [3]. For example, flap reconstruction was used to match cosmetic features such as color, skin thickness, and texture but is rarely utilized in facial burns due to how common simultaneous injuries are to the neck and chest [4].…”

Section: Introductionmentioning

confidence: 99%

3D bioprinted biomask for facial skin reconstruction

et al. 2018

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Skin injury to the face remains one of the greatest challenges in wound care due to the varied contours and complex movement of the face. Current treatment strategies for extensive facial burns are limited to the use of autografts, allografts, and skin substitutes, and these often result in scarring, infection, and graft failure. Development of an effective treatment modality will greatly improve the quality of life and social integration of the affected individuals. In this proof of concept study, we developed a novel strategy, called “BioMask”, which is a customized bioengineered skin substitute combined with a wound dressing layer that snugly fits onto the facial wounds. To achieve this goal, three-dimensional (3D) bioprinting principle was used to fabricate the BioMask that could be customized by patients’ clinical images such as computed tomography (CT) data. Based on a face CT image, a wound dressing material and cell-laden hydrogels were precisely dispensed and placed in a layer-by-layer fashion by the control of air pressure and 3-axis stage. The resulted miniature BioMask consisted of three layers; a porous polyurethane (PU) layer, a keratinocyte-laden hydrogel layer, and a fibroblast-laden hydrogel layer. To validate this novel concept, the bioprinted BioMask was applied to a skin wound on a pre-fabricated face-shaped structure in mice. Through this in vivo study using the 3D BioMask, skin contraction and histological examination showed the regeneration of skin tissue, consisting of epidermis and dermis layers, on the complex facial wounds. Consequently, effective and rapid restoration of aesthetic and functional facial skin would be a significant improvement to the current issues a facial wound patient experience.

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Literature Review Concerning Cell and Skin Substitute Cultures Obtained by Means of Tissue Engineering used in the Treatment of Burns

Cited by 7 publications

References 40 publications

Regenerative Skin Wound Healing in Mammals: State-of-the-Art on Growth Factor and Stem Cell Based Treatments

Regenerative Skin Wound Healing in Mammals: State-of-the-Art on Growth Factor and Stem Cell Based Treatments

The Development of Tissue Engineering Skin

3D bioprinted biomask for facial skin reconstruction

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