Listeria monocytogenes aguA1, but Not aguA2, Encodes a Functional Agmatine Deiminase

Abstract

Background: Listeria monocytogenes has two putative agmatine deiminase homologs, AguA1 and AguA2. Results: Only AguA1, but not AguA2, acts as functional agmatine deiminase and mediates acid tolerance in L. monocytogenes. Conclusion: Provided is the first biological insight into the roles of AgDI in acid tolerance of L. monocytogenes. Significance: We have discovered a novel residue Gly-157 other than the known catalytic triad (Cys-His-Glu/Asp) critical for L. monocytogenes AgDI activity.

Evidence forElizabethkingia anophelisTransmission from Mother to Infant, Hong Kong

“…A homologof the gene encoding agmatine deiminase, AgDI, which mediates acid tolerance in L. monocytogenes ( 33 ), was found in the E. anophelis genomes. Further studies may investigate the possible role of AgDI and potential adherence factors for vaginal colonization in E. anophelis .…”

An M29 Aminopeptidase from Listeria Monocytogenes Contributes to In Vitro Bacterial Growth but not to Intracellular Infection

“…L. monocytogenes gene deletion and complementation strategy was employed as described previously [21,22]. Upstream and downstream regions of the interest gene were overlapped using a homologous recombination strategy with an overlap extension PCR procedure (see the primers in Table 1) and cloned into the plasmid pKSV7.…”

“…, as previously described [21]. The values K m , V max , and K cat were calculated using the software GraphPad Prism 8.0 (GraphPad Software, La Jolla, CA, USA), where [S] is the substrate concentration, v 0 the initial velocity, V max the maximum velocity, and K m the Michaelis-Menten constant.…”

Asidik Koşullar Altında Listeria monocytogenes’in Glutamat Dekarboksilazlarının Asit Direnç Sistemlerine Katkılarının Değerlendirilmesi ve Farklı Rollerinin Araştırılması

“…Listeria monocytogenes contains several enzyme systems including F 0 F 1 -ATPase, ADI, AgDI, GAD and acid tolerance response, to maintain intracellular pH homeostasis in acidic environments [9] . Under acid stress, F 0 F 1 -ATPase system uses ATP hydrolysis to produce proton motive force to pump cytoplasmic protons, while the ADI and AgDI use arginine and agmatine to produce ammonia to neutralize the cytoplasmic protons, respectively [10,11] . The glutamate decarboxylase (GAD) system, which consumes intracellular protons by converting glutamate to γ-aminobutyrate [12] , also plays a role in acid resistance of L. monocytogenes to protect them in low pH foods.…”