Liquid Phase Peptide Synthesis via One‐Pot Nanostar Sieving (PEPSTAR)

Yeo, Jet; Peeva, Ludmila; Chung, Seoyeon; Gaffney, Piers R. J.; Kim, Dae‐Ok; Luciani, Carla V.; Tsukanov, Sergey V.; Seibert, Kevin D.; Kopach, Michael E.; Alberício, Fernando; Livingston, Andrew G.

doi:10.1002/anie.202014445

Cited by 22 publications

(36 citation statements)

References 37 publications

(6 reference statements)

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“…Liquid‐phase synthesis of peptides via one‐potnanostar‐sieving 94,95 . The activated Fmoc‐amino acid structural unit is coupled to the chain end of the H‐peptide‐Nanostar; piperidine eliminates excess amino acids and removes Fmoc; removal of by‐products through membrane permeation; the purified H‐peptide‐nanostar remains was prepared to repeat the loop.…”

Section: Production Methods Of Bioactive Peptidesmentioning

confidence: 99%

“…But it takes a long time and the physical properties of peptide intermediates are difficult to predict (Figure 9). 94–98 …”

Section: Production Methods Of Bioactive Peptidesmentioning

confidence: 99%

“…Compared with SPPS, the material cost is about half saved. The size limit is smaller and the time is shorter 94 …”

Section: Production Methods Of Bioactive Peptidesmentioning

confidence: 99%

“…But it takes a long time and the physical properties of peptide intermediates are difficult to predict (Figure 9). [94][95][96][97][98] Lee et al reported that the prepared C-terminalmodified enkephalin analogs peptides have strong analgesic effects, high efficacy, and high metabolic stability through the method of liquid-phase synthesis. Because of its more convenient operation and higher benefit, it is suitable for large-scale peptide synthesis.…”

Section: Liquid-phase Synthesismentioning

confidence: 99%

“…87 DMF, dimethylformamide; NBP, N-butylpyrrolidinone; SPPS, solid-phase peptide synthesis F I G U R E 9 Liquid-phase synthesis of peptides via one-potnanostar-sieving. 94,95 The activated Fmoc-amino acid structural unit is coupled to the chain end of the H-peptide-Nanostar; piperidine eliminates excess amino acids and removes Fmoc; removal of by-products through membrane permeation; the purified H-peptide-nanostar remains was prepared to repeat the loop. Each process is analyzed in realtime by UHPLC-MS. UHPLC-MS, ultrahigh performance liquid chromatography mass spectrometry with recombinant peptide chimera (CP).…”

Section: Anticancer Peptidesmentioning

confidence: 99%

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Recent research progress of biologically active peptides

et al. 2022

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With the rapid development of molecular biology and biochemical technology, great progress has been made in the study of peptides. Peptides are easy to digest and absorb, with lowering of blood pressure and cholesterol, improving immunity, regulating hormones, antibacterial, and antiviral effects. Peptides also have physiological regulation and biological metabolism functions with applications in the fields of feed production and biomedical research. In the future, the research focus of bioactive peptides will focus on their efficient preparation and application. This article introduces a comprehensive review of the types, synthesis, functionalization, and bio‐related applications of bioactive peptides. For this aim, we introduced in detail various biopeptides and then presented the production methods of bioactive peptides, such as enzymatic synthesis, microbial fermentation, chemical synthesis, and others. The applications of bioactive peptides for anticancers, immune therapy, antibacterial, and other applications have been introduced and discussed. And discussed the development prospects of biologically active peptides.

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Section: Production Methods Of Bioactive Peptidesmentioning

confidence: 99%

“…But it takes a long time and the physical properties of peptide intermediates are difficult to predict (Figure 9). 94–98 …”

Section: Production Methods Of Bioactive Peptidesmentioning

confidence: 99%

“…Compared with SPPS, the material cost is about half saved. The size limit is smaller and the time is shorter 94 …”

Section: Production Methods Of Bioactive Peptidesmentioning

confidence: 99%

Section: Liquid-phase Synthesismentioning

confidence: 99%

Section: Anticancer Peptidesmentioning

confidence: 99%

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Recent research progress of biologically active peptides

et al. 2022

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Benzoyl and Pivaloyl as Efficient Protecting Groups for Controlled Enzymatic Synthesis of DNA and XNA Oligonucleotides

et al. 2022

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Controlled enzymatic synthesis is an alluring alternative to solid-phase synthesis and polymerase-mediated incorporation of nucleotides for the crafting of chemically modified, therapeutic oligonucleotides. While this approach has met some success for the elaboration of long, unmodified DNA sequences, very little research efforts have been dedicated to xeno nucleic acids (XNAs). Here, we have evaluated the possibility of using various 3'-O-blocking groups for controlled synthesis of locked nucleic acids (LNA). LNA nucleosides were equipped with protecting groups used in synthetic organic chemistry and were evaluated for their stability. The most promising candidates, benzoyl-and pivaloyl-protected nucleosides, were converted to the corresponding nucleotides. The resulting modified nucleotides were shown to be accepted by various polymerases. While single incorporation events were observed in high yields, strong esterase activity of polymerases represents a lasting hurdle that needs to be overcome. Overall, this article represents an additional step towards the controlled enzymatic synthesis of LNA-containing oligonucleotides and could be extended to other sugar or nucleobase modified nucleotides.

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A review of recent advances in the use of complex metal nanostructures for biomedical applications from diagnosis to treatment

Hajfathalian,

Mossburg,

Radaic

et al. 2024

WIREs Nanomed Nanobiotechnol

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Complex metal nanostructures represent an exceptional category of materials characterized by distinct morphologies and physicochemical properties. Nanostructures with shape anisotropies, such as nanorods, nanostars, nanocages, and nanoprisms, are particularly appealing due to their tunable surface plasmon resonances, controllable surface chemistries, and effective targeting capabilities. These complex nanostructures can absorb light in the near‐infrared, enabling noteworthy applications in nanomedicine, molecular imaging, and biology. The engineering of targeting abilities through surface modifications involving ligands, antibodies, peptides, and other agents potentiates their effects. Recent years have witnessed the development of innovative structures with diverse compositions, expanding their applications in biomedicine. These applications encompass targeted imaging, surface‐enhanced Raman spectroscopy, near‐infrared II imaging, catalytic therapy, photothermal therapy, and cancer treatment. This review seeks to provide the nanomedicine community with a thorough and informative overview of the evolving landscape of complex metal nanoparticle research, with a specific emphasis on their roles in imaging, cancer therapy, infectious diseases, and biofilm treatment.This article is categorized under: Diagnostic Tools > In Vivo Nanodiagnostics and Imaging Therapeutic Approaches and Drug Discovery > Nanomedicine for Infectious Disease Diagnostic Tools > Diagnostic Nanodevices

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Liquid Phase Peptide Synthesis via One‐Pot Nanostar Sieving (PEPSTAR)

Cited by 22 publications

References 37 publications

Recent research progress of biologically active peptides

Recent research progress of biologically active peptides

Benzoyl and Pivaloyl as Efficient Protecting Groups for Controlled Enzymatic Synthesis of DNA and XNA Oligonucleotides

A review of recent advances in the use of complex metal nanostructures for biomedical applications from diagnosis to treatment

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