Liquid nitrogen pretreatment of eucalyptus sawdust and rice hull for enhanced enzymatic saccharification

Castoldi, Rafael; Corrêa, Vanesa Gesser; Morais, Gutierrez Rodriguês de; Souza, Cristina Giatti Marques de; Bracht, Adelar; Peralta, Rosely A.; Moreira, Regina de Fátima Peralta Muniz

doi:10.1016/j.biortech.2016.11.099

Cited by 29 publications

(13 citation statements)

References 45 publications

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“…The determination was performed with Ni-filtered Cu K α radiation ( λ = 0.154 nm) at 40 kV and 30 mA. Crystallinity index (CrI) was calculated by referring to diffraction intensities of crystalline area and amorphous region according to the following equation [58]:where I cryst is the diffraction intensity of the crystalline peak at about 2 θ = 22°, and I am is the diffraction intensity of the amorphous peak at about 2 θ = 18°.…”

Section: Methodsmentioning

confidence: 99%

Overcoming biomass recalcitrance by synergistic pretreatment of mechanical activation and metal salt for enhancing enzymatic conversion of lignocellulose

Zhang

Huang

et al. 2019

Biotechnol Biofuels

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BackgroundDue to biomass recalcitrance, including complexity of lignocellulosic matrix, crystallinity of cellulose, and inhibition of lignin, the bioconversion of lignocellulosic biomass is difficult and inefficient. The aim of this study is to investigate an effective and green pretreatment method for overcoming biomass recalcitrance of lignocellulose.ResultsAn effective mechanical activation (MA) + metal salt (MAMS) technology was applied to pretreat sugarcane bagasse (SCB), a typical kind of lignocellulosic biomass, in a stirring ball mill. Chlorides and nitrates of Al and Fe showed better synergistic effect with MA, especially AlCl3, ascribing to the interaction between metal salt and oxygen-containing groups induced by MA. Comparative studies showed that MAMS pretreatment effectively changed the recalcitrant structural characteristics of lignocellulosic matrix and reduced the inhibitory action of lignin on enzymatic conversion of SCB. The increase in hydroxyl and carboxyl groups of lignin induced by MAMS pretreatment led to the increase of its hydrophilicity, which could weaken the binding force between cellulase and lignin and reduce the nonproductive binding of cellulase enzymes to lignin.ConclusionsMAMS pretreatment significantly enhanced the enzymatic digestibility of polysaccharides substrate by overcoming biomass recalcitrance without the removal of lignin from enzymatic hydrolysis system.Electronic supplementary materialThe online version of this article (10.1186/s13068-019-1354-6) contains supplementary material, which is available to authorized users.

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Section: Methodsmentioning

confidence: 99%

Overcoming biomass recalcitrance by synergistic pretreatment of mechanical activation and metal salt for enhancing enzymatic conversion of lignocellulose

Zhang

Huang

et al. 2019

Biotechnol Biofuels

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“…The crystallinity of MS increased from 29.15% to 48.41%. The increase of CrI should be attributed to the dissolution of the amorphous region (lignin and hemicellulose) 25–27 . It is well known that the dense structure of eucalyptus is formed by the joint action of cellulose.…”

Section: Resultsmentioning

confidence: 99%

Extension of lubricant drain interval by modified pure biomass oil filter

Chen

Gan

et al. 2021

Asia-Pacific J Chem Eng

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With the extensive use of internal combustion engines, the large volume of lubricant consumption has become an urgent problem. In this study, a new adsorbent of modified sawdust (MS) was used to clean the waste diesel engine lubricant and prepared into a pure biomass oil filter (PBF) for the actual operation of buses. The characterization results of sawdust showed that the crystallinity index of MS enhanced 19.26%, and MS had smoother morphology, obvious pores than raw sawdust (RS). In on‐road tests, both total acid number (1 mg KOH·g−1) and total base number (9 mg KOH·g−1) were within the permitted range of the standard, and the kinematic viscosity showed a decreasing trend. In addition, the concentration of metals and insoluble colloids (<0.2%) in the lubricant was far below the oil change limit, which made the oil drain interval of the two vehicles extended from 15 000 km (recommended by manufacture) to 60 500 and 71 500 km, respectively. Conclusively, it is believed that PBF is efficient to alleviate lubricant deterioration and prolong the oil drain interval.

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“…The high glucose yield in this study may be ascribed to the fact that sequential alkaline extraction not only removed hemicelluloses, but also swelled cellulose macromolecule. Swelling of biomass also occurs during alkaline pretreatment of rice husk with 2% NaOH [31]. However, the successively extracted poplar holocellulose has also yielded an increment of cellulose enzymatic conversion and achieved 61.9% cellulose conversion as the maximum [23].…”

Section: Enzymatic Hydrolysis Of Cellulose-rich Substratesmentioning

confidence: 99%

Enzymatic response of ryegrass cellulose and hemicellulose valorization introduced by sequential alkaline extractions

Sun

Yang

Wang

et al. 2021

Biotechnol Biofuels

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Background In view of the natural resistance of hemicelluloses in lignocellulosic biomass on bioconversion of cellulose into fermentable sugars, alkali extraction is considered as an effective method for gradually fractionating hemicelluloses and increasing the bioconversion efficiency of cellulose. In the present study, sequential alkaline extractions were performed on the delignified ryegrass material to achieve high bioconversion efficiency of cellulose and comprehensively investigated the structural features of hemicellulosic fractions for further applications. Results Sequential alkaline extractions removed hemicelluloses from cellulose-rich substrates and degraded part of amorphous cellulose, reducing yields of cellulose-rich substrates from 73.0 to 27.7% and increasing crystallinity indexes from 31.7 to 41.0%. Alkaline extraction enhanced bioconversion of cellulose by removal of hemicelluloses and swelling of cellulose, increasing of enzymatic hydrolysis from 72.3 to 95.3%. In addition, alkaline extraction gradually fractionated hemicelluloses into six fractions, containing arabinoxylans as the main polysaccharides and part of β-glucans. Simultaneously, increasing of alkaline concentration degraded hemicellulosic polysaccharides, which resulted in a decreasing their molecular weights from 67,510 to 50,720 g/mol. Conclusions The present study demonstrated that the sequential alkaline extraction conditions had significant effects on the enzymatic hydrolysis efficiency of cellulose and the investigation of the physicochemical properties of hemicellulose. Overall, the investigation the enzymatic hydrolysis efficiency of cellulose-rich substrates and the structural features of hemicelluloses from ryegrass will provide useful information for the efficient utilization of cellulose and hemicelluloses in biorefineries.

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Liquid nitrogen pretreatment of eucalyptus sawdust and rice hull for enhanced enzymatic saccharification

Cited by 29 publications

References 45 publications

Overcoming biomass recalcitrance by synergistic pretreatment of mechanical activation and metal salt for enhancing enzymatic conversion of lignocellulose

Overcoming biomass recalcitrance by synergistic pretreatment of mechanical activation and metal salt for enhancing enzymatic conversion of lignocellulose

Extension of lubricant drain interval by modified pure biomass oil filter

Enzymatic response of ryegrass cellulose and hemicellulose valorization introduced by sequential alkaline extractions

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