Liquid chromatography/tandem mass spectrometry for the determination of carbamazepine and its main metabolite in rat plasma utilizing an automated blood sampling system

Zhang, Yongxin; Chiang, Hwa; Wulster-Radcliffe, Meghan; Hilt, Rita; Wong, Philip; Kissinger, Candice B.; Kissinger, Peter T.

doi:10.1016/j.jpba.2004.11.058

Cited by 43 publications

(19 citation statements)

References 27 publications

(25 reference statements)

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“…Such as in studies performed in animals administrated with only one of the drugs [36,39], in the present study, both ESL and CBZ was demonstrated to be rapidly absorbed. Moreover, and as it was expected considering that ESL is a prodrug of S-Lic, ESL was quickly metabolized and no parent compound was detected in plasma at 1 h post dosing.…”

Section: Methods Applicationsupporting

confidence: 72%

A chiral HPLC‐UV method for the quantification of dibenz[b,f]azepine‐5‐carboxamide derivatives in mouse plasma and brain tissue: Eslicarbazepine acetate, carbamazepine and main metabolites

Fortuna

Bicker

Alves

et al. 2011

J of Separation Science

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For the first time, a selective and sensitive chiral HPLC-UV method was developed and fully validated for the simultaneous quantification of eslicarbazepine acetate (ESL), carbamazepine (CBZ), S-licarbazepine (S-Lic), R-licarbazepine (R-Lic), oxcarbazepine (OXC) and carbamazepine-10,11-epoxide (CBZ-E), in mouse plasma and brain homogenate supernatant. After the addition of chloramphenicol as the internal standard, samples were processed using an SPE procedure. The chiral chromatographic analysis was carried out on a LiChroCART 250-4 ChiraDex column, employing a mobile phase of water and methanol (88:12, v/v) pumped at 0.9 mL/min and the UV detector set at 235 nm. The assay was linear (r(2) ≥0.995) for ESL, CBZ, OXC, S-Lic, R-Lic and CBZ-E in the range of, respectively, 0.2-4, 0.4-30, 0.1-60, 0.2-60, 0.2-60 and 0.2-30 μg/mL, in plasma, and of 0.06-1.5 μg/mL for ESL, 0.12-15 μg/mL for CBZ and CBZ-E and 0.06-15 μg/mL for OXC and both licarbazepine (Lic) enantiomers in brain homogenate supernatant. The overall precision was within 8.71% and accuracy ranged from -7.55 to 8.97%. The recoveries of all the compounds were over 92.1%. Afterwards, the application of the method was demonstrated using real plasma and brain samples obtained from mice administered simultaneously with ESL and CBZ.

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Section: Methods Applicationsupporting

confidence: 72%

A chiral HPLC‐UV method for the quantification of dibenz[b,f]azepine‐5‐carboxamide derivatives in mouse plasma and brain tissue: Eslicarbazepine acetate, carbamazepine and main metabolites

Fortuna

Bicker

Alves

et al. 2011

J of Separation Science

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“…The extraction method for carbamazepine utilized liquid-liquid extraction (LLE) with ethyl acetate. The extraction method was modified from the procedure described by Zhu et al (19). Following sample dilution with 0.1 ml water, 2 ml of ethyl acetate was added to each sample and then shaken by vortex for 1 min.…”

Section: Plasma Sample Preparationmentioning

confidence: 99%

Microdosing Assessment to Evaluate Pharmacokinetics and Drug Metabolism in Rats Using Liquid Chromatography-Tandem Mass Spectrometry

Ouyang

Aiello³

et al. 2008

Pharm Res

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“…Zhu et al [17] have investigated the fragmentation behaviour of carbamazepine and its epoxide metabolite by ESI-MS/ MS in the positive ion mode using selected reaction monitoring (SRM). Carbamazepine was monitored using the transition m/z 237 ?…”

Section: Carbamazepinementioning

confidence: 99%

Recent applications of capillary electrophoresis‐electrospray ionisation‐mass spectrometry in drug analysis

Smyth

2006

Electrophoresis

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This review considers applications in 2004-2005 of capillary electrophoresis-electrospray ionisation-mass spectrometry (CE-ESI-MS) to the detection and determination of small molecular mass drug molecules, taken from the Web of Knowledge database. The molecules of small molecular mass less than 1000 Da are chosen according to selected structural classes in which they give ESI signals primarily as [M + H](+) ions. These structural classes are drugs with amine-containing side chains, drugs with N-containing saturated ring structures, 1,4-benzodiazepines, other heterocyclic hypnotics, steroids, bioactive compounds containing phenolic groups, and miscellaneous molecules. Details are given on the fragmentations, where available, that these ionic species exhibit in-source and in ion-trap, triple quadrupole and time-of flight mass spectrometers. The review then gives a critical evaluation of these recent CE-ESI-MS analytical methods for the detection and determination of these small molecular mass drug molecules. Analytical information on, for example, sample concentration techniques, CE separation conditions, recoveries from biological media and limits of detection are provided.

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Liquid chromatography/tandem mass spectrometry for the determination of carbamazepine and its main metabolite in rat plasma utilizing an automated blood sampling system

Cited by 43 publications

References 27 publications

A chiral HPLC‐UV method for the quantification of dibenz[b,f]azepine‐5‐carboxamide derivatives in mouse plasma and brain tissue: Eslicarbazepine acetate, carbamazepine and main metabolites

A chiral HPLC‐UV method for the quantification of dibenz[b,f]azepine‐5‐carboxamide derivatives in mouse plasma and brain tissue: Eslicarbazepine acetate, carbamazepine and main metabolites

Microdosing Assessment to Evaluate Pharmacokinetics and Drug Metabolism in Rats Using Liquid Chromatography-Tandem Mass Spectrometry

Recent applications of capillary electrophoresis‐electrospray ionisation‐mass spectrometry in drug analysis

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