Liquid chromatography–tandem mass spectrometry assay for human serum testosterone and trideuterated testosterone

Detection, quantification and confirmation of anabolic steroids in equine plasma by liquid chromatography and tandem mass spectrometry

“…With the advance of LC-MS technology, the emphasis has shifted in favor of this new technology. There are reports of LC-MS methods for quantitative determination of anabolic steroids such as testosterone, nandrolone, and trenbolone in human or bovine plasma or serum [16][17][18][19], in bovine or equine or human urine [20][21][22][23][24][25][26][27][28] and in human saliva [29][30][31]. To our knowledge, there is no report on LC-MS methods for qualitative and quantitative determination of anabolic steroids in equine plasma.…”

Time-Resolved Fluoroimmunoassay for 19-Nortestosterone Residues in Aquaculture Tissues

“…Recently, LC/MS/MS has been successfully applied to the analysis of ASs in various biological samples including urine from bovine and horse, bovine hair and kidney fat, [8][9][10][11][12][13][14][15][16] but few applications of the methods on muscular tissue of aquaculture have been reported. The limits of detection (LOD) of LC/MS/MS were above 0.1 ng g -1 , [17][18][19][20][21][22][23][24] and the sample preparation was trivial. [25][26][27] In China, inspection of muscular tissue of animals plays an important role.…”

Determination of testosterone concentrations in rat plasma using liquid chromatography–atmospheric pressure chemical ionization mass spectrometry combined with ethyl oxime and acetyl ester derivatization

“…RIAs are simple, rapid, and inexpensive, but crossreactivity in these assays is of great concern [1]. In contrast, chromatography-coupled mass spectrometric methods, especially LC-MS/MS methods, have high specificity and involve little or no crossreactivity [1][2][3][4].…”

“…As a result, smaller plasma volumes are available for analysis. Many of the reported LC-MS methods have been developed for the analysis of human serum or plasma [1,2], and subsequently they utilize serum or plasma sample volumes around 1 ml or larger. The only exception to these LC-MS methods that use relatively large sample volumes is that reported by Zhao et al [3].…”