Liquid chromatography–mass spectrometry identification of anthocyanins of isla oca (Oxalis tuberosa, Mol.) tubers

Alcalde-Eon, Cristina; Saavedra, Gloria; Pascual‐Teresa, Sonia de; Rivas-Gonzalo, Julián C.

doi:10.1016/j.chroma.2004.08.074

Cited by 21 publications

(1 citation statement)

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“…Unlike the anthocyanins present in different fruits and vegetables, the anthocyanins of red cabbage leaves are highly conjugated with a variety of sugars and/or acyl groups; with the lack of pure standards, it would be a challenge to fulfill their characterization and identification [1][2][3][4]. Several studies on the characterization and identification of secondary metabolites in complex plant extracts showed that liquid chromatography coupled to photodiode array detection is very effective for anthocyanins analysis [5,6].…”

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confidence: 99%

High-Resolution UPLC-MS Profiling of Anthocyanins and Flavonols of Red Cabbage (Brassica oleracea L. var. capitata f. rubra DC.) Cultivated in Egypt and Evaluation of Their Biological Activity

2021

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In this paper, biological investigations and a high-resolution UPLC-PDA-ESI-qTOF-HRMS technique were employed for Brassica oleracea L. var. capitata f. rubra DC. (red cabbage) of the family Brassicaceae (Cruciferae), cultivated in Egypt, for the first time. The positive ionization mode is usually performed to identify anthocyanins. However, this technique cannot differentiate between anthocyanins and corresponding non-anthocyanin polyphenols. Thus, the negative ionization mode was also used, as it provided a series of characteristic ions for the MS analysis of anthocyanins. This helped in identifying five kaempferol derivatives for the first time in red cabbage, as well as nine—previously reported—anthocyanins. For the biological investigations, the acidified methanolic extract of fresh leaves and the methanolic extract of air-dried powdered leaves were examined for their antioxidant, antimicrobial, and anticancer activities. The freshly prepared phenolic extract was proven to be more biologically potent. Statistical significance was determined for its anticancer activity in comparison with standard doxorubicin.

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