Liquid Chromatographic Determination of Dehydroepiandrosterone (DHEA) in Dietary Supplement Products

Abstract

A liquid chromatographic (LC) method was developed to assess the potency of products that contain dehydroepiandrosterone (DHEA), a precursor hormone synthesized from cholesterol by the human adrenal cortex and converted to potent androgens and/or estrogens in peripheral tissues. Forty-five commercial products (both single and multi-ingredient) were subjected to analysis by the proposed method. A Zorbax Rx C18 column with a mobile phase containing acetonitrile–0.025M phosphate buffer (60 + 40), pH 3.50, and UV detection at 292 nm was used for 87% of the products. An alternative mobile phase containing methanol–0.025M phosphate (75 + 25), pH 3.50, was used to isolate DHEA from more complex product mixtures. Assay values varied from 0 to 109.5% of the declared amount with an overall mean value of 91.1%. The recoveries based on fortified products ranged from 96.4 to 101.2%, and the intraday precision (RSD, n = 5) varied from 0.50 to 1.66%.

A meta‐analysis of dehydroepiandrosterone supplementation among women with diminished ovarian reserve undergoing in vitro fertilization or intracytoplasmic sperm injection

“…As serum levels of DHEA decline with age, both premenopausal and postmenopausal women experience a lowered libido, diminished sexual function, and a loss of well‐being [7,8]. As an empirical treatment, DHEA is widely used for its anti‐aging and performance‐boosting effects [9]. It has also gained popularity as a medication to improve ovarian response in poor responders, especially women with DOR, during IVF/ICSI treatment.…”

Development and validation of a simple solid‐phase extraction method coupled with liquid chromatography–triple quadrupole tandem mass spectrometry for simultaneous determination of lincomycin, tylosin A and tylosin B in royal jelly

“…Moreover, a column with a size of 2.1 × 150 mm was employed in order to ensure that the antibiotics are detectable after 1 min. Further, the following composi- (Thompson & Carlson, 2000). 1993).…”

Residue analysis of tebufenozide and indoxacarb in chicken muscle, milk, egg and aquatic animal products using liquid chromatography–tandem mass spectrometry

“…We chose the last combination, as it produced the strongest signals. Filtration and reconstitution in methanol prior to LC-MS/MS analysis are generally necessary to protect the instrument and the column (Thompson & Carlson, 2000).…”