Lipophorin: A hemolymph juvenile hormone binding protein in the german cockroach, Blattella germanica

Site of synthesis, tissue distribution, and lipophorin transport of hydrocarbons in Blattella germanica (L.) nymphs

“…Because its principal functions change during development, it is unlikely that the lipophorin titer will correspond with any one of its ligands. For example, lipophorin carries juvenile hormone (Trowell, 1992;Sevala et al, 1997), but its titer in the hemolymph has no relation to the juvenile hormone concentration (King and Tobe, 1993;Sevala et al, 1999). This is supported by our current work with last instars.…”

“…High density lipophorin was purified from adult female B. germanica using KBr gradient ultracentrifugation (Gu et al, 1995;Sevala et al, 1997). Lipophorin polyclonal antibodies were raised in New Zealand White rabbits and radial imunodiffusion assays confirmed specificity to lipophorin and no cross-reactivity with other hemolymph proteins (Sevala et al, 1999).…”

Structural and RNAi characterization of the German cockroach lipophorin receptor, and the evolutionary relationships of lipoprotein receptors

“…Our experiments also showed that JH-treated specimens had higher levels of lipophorin, estimated in terms of apolipophorin I (ApoLpI). However, this likely derive from the increased levels of BgLpR, given that JH do not seem to regulate lipophorin levels directly [4,5], although in the ovary, higher levels of ApoLpI can also be due to the enhanced basal oocyte growth provoked by the treatment with JH.…”

“…However, lipophorin has additionally been related to other functions, for example, it plays a role as juvenile hormone (JH) binding protein in certain species, which suggests that it may contribute to the regulation of JH titers in the hemolymph [4,5]. During vitellogenesis, lipophorin transports lipids and other yolk precursors from the fat body to the ovaries and, in some species, lipophorin itself takes part of the egg yolk platelets [6].…”

A Comparative Protein Profile of Virgin and Mated Male of Leucinodes Orbonalis Accessory Gland

“…Lipophorins are insect proteins, which are dispersed in the hemolymph and involved in lipid transport, acting as a shuttle of lipids between different tissues (Ryan & Horst, 2000). Sevala et al 1997Sevala et al , (1999 demonstrated in Blattella germanica revealed that lipophorins also play a role as juvenile hormone (JH) binding protein and suggests that it may contribute to the regulation of JH titers in the hemolymph. It was also suggested that in case of two triatomine species, lipophorins play a role as yolk protein, being endocytosed by the oocytes, stored in yolk bodies and used to support embryonic development (Kawooya and Law, 1988;Ziegler and Van Antwerpen, 2006;Fruttero et al, 2011Fruttero et al, , 2017Leyria et al, 2014).…”