Lipoic acid in combination with a chelator ameliorates lead-induced peroxidative damages in rat kidney

Abstract

The deleterious effect of lead has been attributed to lead-induced oxidative stress with the consequence of lipid peroxidation. The present study was designed to investigate the combined effect of DL-alpha-lipoic acid (LA) and meso-2,3-dimercaptosuccinic acid (DMSA) on lead-induced peroxidative damages in rat kidney. The increase in peroxidated lipids in lead-poisoned rats was accompanied by alterations in antioxidant defence systems. Lead acetate (Pb, 0.2%) was administered in drinking water for 5 weeks to induce lead toxicity. LA (25 mg/kg body weight per day i.p.) and DMSA (20 mg/kg body weight per day i.p.) were administered individually and also in combination during the sixth week. Nephrotoxic damage was evident from decreases in the activities of gamma-glutamyl transferase and N-acetyl beta- D-glucosaminidase, which were reversed upon combined treatment with LA and DMSA. Rats subjected to lead intoxication showed a decline in the thiol capacity of the cell, accompanied by high malondialdehyde levels along with lowered activities of catalase, superoxide dismutase, glutathione peroxidase and glutathione metabolizing enzymes (glutathione reductase, glucose-6-phosphate dehydrogenase, glutathione- S-transferase). Supplementation with LA as a sole agent showed considerable changes over oxidative stress parameters. The study has highlighted the combined effect of both drugs as being more effective in reversing oxidative damage by bringing about an improvement in the reductive status of the cell.

Renal effects and erythrocyte oxidative stress in long-term low-level lead-exposed adolescent workers in auto repair workshops

“…Monterio et al (1985) showed that individuals exposed to lead have increased antioxidant defenses (SOD and GPX), and they found a nonlinear relationship between Pb-B and SOD. Recently Sivaprasad et al (2002) also reported a decrease in the concentrations of reduced glutathione and an increase in the levels of oxidized glutathione and MDA in lead acetate treated rats. Both experimental and clinical studies reported for years that lead exposure in the acceptable intoxication ranges can cause oxidative damage.…”

Endoplasmic Reticulum Stress and Apoptosis Triggered by Sub-Chronic Lead Exposure in Mice Spleen: a Histopathological Study

“…It is known that Pb causes oxidative stress by generating ROS in different organs [51][52][53][54][55]. Sub-chronic low Pb exposure produces nephrotoxicity in vivo and in vitro [36], as well as infertility [56] in rodents.…”

Evaluating the Effect of Melillotus officinalis L. Aqueous Extracts on Healing of Acetic Acid-Induced Ulcerative Colitis in Male Rats

“…Furthermore, studies have clearly demonstrated that depletion of GSH leads to the cellular damage as well as colonic injuries. GSH is one of the first lines of oxidative defense mechanisms against free radicals (41), and can prevent ROS oxidative injuries (42,43).…”