Lipids of human atheroma Part 4. Characterisation of a new group of polar sterol esters from human atherosclerotic plaques

Role of Protein Kinase C Isoforms in the Regulation of Interleukin-13-induced 15-Lipoxygenase Gene Expression in Human Monocytes

“…LTB 4 Production by Monocytes-Monocytes (1 ϫ 10 6 cells/ml) were plated in duplicate in 12-well culture plates and either pretreated with 5 M rottlerin for 30 min or left without rottlerin before IL-13 treatment for 72 h. Cells were then challenged with the Ca 2ϩ -ionophore A23187, at a final concentration of 5 M for 15 min. At the end of the incubation, cell supernatants were collected and assayed for LTB 4 using the LTB 4 Biotrak enzyme immunoassay (EIA) system (Amersham Biosciences) following the manufacturer's protocol.…”

“…At the end of the incubation, cell supernatants were collected and assayed for LTB 4 using the LTB 4 Biotrak enzyme immunoassay (EIA) system (Amersham Biosciences) following the manufacturer's protocol.…”

“…3, A and B, where pharmacological inhibitors were employed. 4 ProductionPrevious studies suggest that LTB 4 production in human monocytes is inhibited by IL-4-induced 15-LO expression (10). We reproduced this observation with IL-13-induced 15-LO expression and tested whether a blockade of IL-13-induced 15-LO expression by the PKC␦ inhibitor rottlerin could restore LTB 4 production.…”

“…It is believed that 15-LO products play an important role in the pathogenesis of atherosclerosis and other inflammatory diseases (4 -7). In addition to its pro-inflammatory actions, 15-LO has also been shown to suppress the inflammatory responses by inhibiting the production of the potent chemotactic factor LTB 4 . This process has been observed in several cell types, including human monocytes (8 -10).…”

Probucol Reduces Oxysterol Formation in Hypertensive Rabbits

“…[11][12][13][14][15][16] Because cholesterol oxides (ChOx) have been ubiquitously identified in human plasma and atheromatous lesions, their involvement in the atherogenic process is receiving increasing scrutiny. [17][18][19][20][21] ChOx may influence the atherogenic process in several ways, since they are cytotoxic toward the cellular components of the arterial wall, [22][23][24] increase vascular permeability, 25 promote cholesterol ester formation and accumulation, 26 -28 inhibit prostaglandin I 2 synthesis by endothelial cells, 29 and perturb cholesterol biosynthesis. 30,31 Previously, we and others have shown that cholesterol feeding of New Zealand White rabbits leads to increased plasma and aortic wall cholesterol oxide content, 7,32 which is reduced with the antioxidant agent probucol.…”