Lipidomic “Deep Profiling”: An Enhanced Workflow to Reveal New Molecular Species of Signaling Lipids

Narayanaswamy, Pradeep; Shinde, Sudhirkumar; Šulc, Robert; Kraut, Rachel; Staples, Gregory O.; Thiam, Chung Hwee; Grimm, Rudolf; Sellergren, Börje; Torta, Federico; Wenk, Markus R.

doi:10.1021/ac4039652

Cited by 77 publications

(67 citation statements)

References 14 publications

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“…Menuz et al, were able to detect C17iso sphingosine-1-phosphate and also found a 10-fold increase in sphk-1 mutants [42]. Potentially novel sample preparation methods can overcome this limitation, which also enabled detection new forms of S1P in Drosophila [89]. Lastly, direct detection and identification of intact lipids using either shotgun or LC-MS based lipidomics will replace long isolation, derivatization and measurement of fatty acids and will lead to new insights.…”

Section: Challenges and Future Research Directionsmentioning

confidence: 99%

The Caenorhabditis elegans lipidome

Witting

Schmitt‐Kopplin

2016

Archives of Biochemistry and Biophysics

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Section: Challenges and Future Research Directionsmentioning

confidence: 99%

The Caenorhabditis elegans lipidome

Witting

Schmitt‐Kopplin

2016

Archives of Biochemistry and Biophysics

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“…Wenk et al . have selectively extracted phospho-monoester lipids by an imidazolium polymer, and after derivatization with trimethylsilyldiazomethane (TMS-diazomethane), they successfully determined the long-chain base phosphates (LCB-Ps, e.g., sphingosine-1-phosphate) [29]. Moreover, this is particularly effective for selectively extracting some lipids on the basis of their discrepancy in the adsorption capability on solid phase extraction columns through different eluents [30,31,32].…”

Section: The Methods For Lipid Extractionmentioning

confidence: 99%

Mass Spectrometry Methodology in Lipid Analysis

Lin

Han

Wang

et al. 2014

IJMS

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Lipidomics is an emerging field, where the structures, functions and dynamic changes of lipids in cells, tissues or body fluids are investigated. Due to the vital roles of lipids in human physiological and pathological processes, lipidomics is attracting more and more attentions. However, because of the diversity and complexity of lipids, lipid analysis is still full of challenges. The recent development of methods for lipid extraction and analysis and the combination with bioinformatics technology greatly push forward the study of lipidomics. Among them, mass spectrometry (MS) is the most important technology for lipid analysis. In this review, the methodology based on MS for lipid analysis was introduced. It is believed that along with the rapid development of MS and its further applications to lipid analysis, more functional lipids will be identified as biomarkers and therapeutic targets and for the study of the mechanisms of disease.

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“…The quantification of S1P is challenging due to its zwitterion character, even more so the determination of relatively low levels in a complex matrix of other lipids. Presently, the most used detection methods are based on HPLC separation of fluorescently modified S1P [23,24] and LC-MS/MS procedures [25,26]. In addition to the analytical challenge, the origin of S1P in plasma samples and its stability during sample storage warrant special attention.…”

Section: Introductionmentioning

confidence: 99%