Lipidome-wide disturbances of human placental JEG-3 cells by the presence of MEHP

Petit, Julia; Wakx, Anaïs; Gil, Sophie; Fournier, Thierry; Auzeil, Nicolas; Rat, Patrice; Laprévôte, Olivier

doi:10.1016/j.biochi.2018.03.002

Cited by 30 publications

(22 citation statements)

References 63 publications

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“…These compounds have been demonstrated to cross the human placenta and reach the umbilical cord [ 81 ] and the amniotic fluid [ 82 ]. The altered placental development might be due to a peroxisome proliferator-activated receptor (PPAR)γ-mediated disruption in placental lipid metabolism, accounting for modified glycerolipids and glycerophospholipids levels, with a marked accumulation of triacylglycerols [ 83 ]. Furthermore, the presence of DEHP was also detected in maternal milk; thus, exposing newborns to these contaminants during breast feeding [ 84 ].…”

Section: Toxicological Aspects and Human Health Effectsmentioning

confidence: 99%

Critical Review on the Presence of Phthalates in Food and Evidence of Their Biological Impact

Giuliani

Zuccarini

Cichelli

et al. 2020

IJERPH

230

119

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Phthalates are a huge class of chemicals with a wide spectrum of industrial uses, from the manufacture of plastics to food contact applications, children’s toys, and medical devices. People and animals can be exposed through different routes (i.e., ingestion, inhalation, dermal, or iatrogenic exposure), as these compounds can be easily released from plastics to water, food, soil, air, making them ubiquitous environmental contaminants. In the last decades, phthalates and their metabolites have proven to be of concern, particularly in products for pregnant women or children. Moreover, many authors reported high concentrations of phthalates in soft drinks, mineral waters, wine, oil, ready-to-eat meals, and other products, as a possible consequence of their accumulation along the food production chain and their accidental release from packaging materials. However, due to their different physical and chemical properties, phthalates do not have the same human and environmental impacts and their association to several human diseases is still under debate. In this review we provide an overview of phthalate toxicity, pointing out the health and legal issues related to their occurrence in several types of food and beverage.

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Section: Toxicological Aspects and Human Health Effectsmentioning

confidence: 99%

Critical Review on the Presence of Phthalates in Food and Evidence of Their Biological Impact

Giuliani

Zuccarini

Cichelli

et al. 2020

IJERPH

230

119

View full text Add to dashboard Cite

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“…Jukic et al revealed that pregnant females' exposure to excess di(2-ethylhexyl) phthalate (DEHP) was believed to increase the risk of developing early embryo loss [109]. MEHP changed the synthesis of lipidosomes in the JEG-3 human trophoblast cells leading to lipid imbalance [110]. Furthermore, Grindler et al investigated the methylation changes of 39 genes in first trimester placenta and found that most of the changes were negative [105].…”

Section: Phthalatesmentioning

confidence: 99%

Oestrogenic Endocrine Disruptors in the Placenta and the Fetus

Tang

Deng

et al. 2020

IJMS

124

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Endocrine disrupting chemicals (EDCs) are exogenous substances that interfere with the stability and regulation of the endocrine system of the body or its offspring. These substances are generally stable in chemical properties, not easy to be biodegraded, and can be enriched in organisms. In the past half century, EDCs have gradually entered the food chain, and these substances have been frequently found in maternal blood. Perinatal maternal hormone levels are unstable and vulnerable to EDCs. Some EDCs can affect embryonic development through the blood-fetal barrier and cause damage to the neuroendocrine system, liver function, and genital development. Some also effect cross-generational inheritance through epigenetic mechanisms. This article mainly elaborates the mechanism and detection methods of estrogenic endocrine disruptors, such as bisphenol A (BPA), organochlorine pesticides (OCPs), diethylstilbestrol (DES) and phthalates (PAEs), and their effects on placenta and fetal health in order to raise concerns about the proper use of products containing EDCs during pregnancy and provide a reference for human health.

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“…In human cells, exposure to monoethylhexyl phthalate (MEHP) affected adipocyte size, possibly via increased lipolysis, glucose uptake, and oxygen consumption [31]. In placental cells MEHP produced lipidome-wide changes with specific alterations in glycerolipids and glycerophospholipids and promoted triacylglycerols accumulation [34]. Interestingly, prenatal DEHP exposure in rats was shown to target adrenal gland peroxisome proliferator-activated receptor (PPAR) and cholesterol biosynthesis pathways [33], which rendered offspring more susceptibility to second-hit stressor challenge [32].…”

Section: Discussionmentioning

confidence: 99%

Tissue-specific changes in Srebf1 and Srebf2 expression and DNA methylation with perinatal phthalate exposure

Moody

Hernández-Saavedra

Kougias

et al. 2019

Environmental Epigenetics

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Perinatal exposure to endocrine disrupting chemicals negatively impacts health, but the mechanism by which such toxicants damage long-term reproductive and metabolic function is unknown. Lipid metabolism plays a pivotal role in steroid hormone synthesis as well as energy utilization and storage; thus, aberrant lipid regulation may contribute to phthalate-driven health impairments. In order to test this hypothesis, we specifically examined epigenetic disruptions in lipid metabolism pathways after perinatal phthalate exposure. During gestation and lactation, pregnant Long–Evans rat dams were fed environmentally relevant doses of phthalate mixture: 0 (CON), 200 (LO), or 1000 (HI) µg/kg body weight/day. On PND90, male offspring in the LO and HI groups had higher body weights than CON rats. Gene expression of lipid metabolism pathways was altered in testis and adipose tissue of males exposed to the HI phthalate dosage. Specifically, Srebf1 was downregulated in testis and Srebf2 was upregulated in adipose tissue. In testis of HI rats, DNA methylation was increased at two loci and reduced at one other site surrounding Srebf1 transcription start site. In adipose tissue of HI rats, we observed increased DNA methylation at one region within the first intron of Srebf2 . Computational analysis revealed several potential transcriptional regulator binding sites, suggesting functional relevance of the identified differentially methylated CpGs. Overall, we show that perinatal phthalate exposure affects lipid metabolism gene expression in a tissue-specific manner possibly through altering DNA methylation of Srebf1 and Srebf2 .

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Lipidome-wide disturbances of human placental JEG-3 cells by the presence of MEHP

Cited by 30 publications

References 63 publications

Critical Review on the Presence of Phthalates in Food and Evidence of Their Biological Impact

Critical Review on the Presence of Phthalates in Food and Evidence of Their Biological Impact

Oestrogenic Endocrine Disruptors in the Placenta and the Fetus

Tissue-specific changes in Srebf1 and Srebf2 expression and DNA methylation with perinatal phthalate exposure

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Lipidome-wide disturbances of human placental JEG-3 cells by the presence of MEHP

Cited by 30 publications

References 63 publications

Critical Review on the Presence of Phthalates in Food and Evidence of Their Biological Impact

Critical Review on the Presence of Phthalates in Food and Evidence of Their Biological Impact

Oestrogenic Endocrine Disruptors in the Placenta and the Fetus

Tissue-specific changes in Srebf1 and Srebf2 expression and DNA methylation with perinatal phthalate exposure

Contact Info

Product

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Lipidome-wide disturbances of human placental JEG-3 cells by the presence of MEHP