Lipid Regulation of Acrosome Exocytosis

Cohen, Roy; Mukai, Chinatsu; Travis, Alexander J.

doi:10.1007/978-3-319-30567-7_6

Cited by 19 publications

(16 citation statements)

References 68 publications

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“…Consequently, cellular membrane integrity can be maintained by balancing the physiological cholesterol/phospholipid ratio [ 45 ]. Numerous studies indicate that the interaction between cholesterol and phospholipid could prevent the premature capacitation through spermatozoa membrane stabilization [ 46 ]. Vesicles (including exosomes) could inhibit progression to late capacitation events and avoid premature acrosome reaction by binding or fusion to sperm membrane early during capacitation [ 11 ].…”

Section: Discussionmentioning

confidence: 99%

Boar seminal plasma exosomes maintain sperm function by infiltrating into the sperm membrane

et al. 2016

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Seminal plasma ingredients are important for maintenance of sperm viability. This study focuses on the effect of boar seminal plasma exosomes on sperm function during long-term liquid storage. Boar seminal plasma exosomes had typical nano-structure morphology as measured by scanning electron microscopy (SEM) and molecular markers such as AWN, CD9 and CD63 by western blot analysis. The effect on sperm parameters of adding different ratio of boar seminal plasma exosomes to boar sperm preparations was analyzed. Compared to the diluent without exosomes, the diluent with four times or sixteen times exosomes compared to original semen had higher sperm motility, prolonged effective survival time, improved sperm plasma membrane integrity (p < 0.05), increased total antioxidant capacity (T-AOC) activity and decreased malondialdehyde (MDA) content. The diluent containing four times concentration of exosomes compared to original semen was determined to inhibit premature capacitation, but not to influence capacitation induced in vitro. Inhibition of premature capacitation is likely related to the concentration of exosomes which had been demonstrated to transfer proteins including AWN and PSP-1 into sperm. In addition, using fluorescence microscopy and scanning electron microscopy analysis, it was demonstrated that exosomes in diluent were directly binding to the membrane of sperm head which could improve sperm plasma membrane integrity.

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Section: Discussionmentioning

confidence: 99%

Boar seminal plasma exosomes maintain sperm function by infiltrating into the sperm membrane

et al. 2016

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“…Thus, this switch in the PIP state of the cilia may favor certain GPCRs or channels within cilia (Chávez et al, 2015; Garcia-Gonzalo et al, 2015). Lipid remodeling is a crucial part of sperm physiology, and cholesterol efflux is a marker of sperm capacitation (Cohen et al, 2016; Stival et al, 2016). Except for sperm flagella, the relative abundance of other lipids in the cilium’s membrane, such as cholesterol, is not well known.…”

Section: Ciliary Structure and Functionmentioning

confidence: 99%

Progress in ciliary ion channel physiology

Pablo

DeCaen

Clapham

2016

Journal of General Physiology

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Mammalian cilia are ubiquitous appendages found on the apical surface of cells. Primary and motile cilia are distinct in both morphology and function. Most cells have a solitary primary cilium (9+0), which lacks the central microtubule doublet characteristic of motile cilia (9+2). The immotile primary cilia house unique signaling components and sequester several important transcription factors. In contrast, motile cilia commonly extend into the lumen of respiratory airways, fallopian tubes, and brain ventricles to move their contents and/or produce gradients. In this review, we focus on the composition of putative ion channels found in both types of cilia and in the periciliary membrane and discuss their proposed functions. Our discussion does not cover specialized cilia in photoreceptor or olfactory cells, which express many more ion channels.

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“…These changes in lipid compositions in SM are essential for membrane fusion and are required for the bending and fusion of the membrane domains in the plasma membrane and organelles, while recruiting proteins from various membranes and the cytosol. The SM also exert appropriate control over signal processing mediators, such as calcium ions (Ca 2+ ) [ 15 ]. Lipids from different SM are subject to exchange and renewal by various mechanisms, such as endocytosis, exocytosis, contacts between different membranes and non-membrane lipid vesicles and by non-vesicular lipid carrier trafficking [ 15 , 16 ].…”

Section: Introductionmentioning

confidence: 99%

“…The SM also exert appropriate control over signal processing mediators, such as calcium ions (Ca 2+ ) [ 15 ]. Lipids from different SM are subject to exchange and renewal by various mechanisms, such as endocytosis, exocytosis, contacts between different membranes and non-membrane lipid vesicles and by non-vesicular lipid carrier trafficking [ 15 , 16 ]. For example, the compositions of lipids in the SM have been shown to be susceptible to compositional changes by the incorporation/fusion of nanoliposomes [ 17 ].…”

Section: Introductionmentioning

confidence: 99%

Incubation of human sperm with micelles made from glycerophospholipid mixtures increases sperm motility and resistance to oxidative stress

et al. 2018

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Membrane integrity is essential in maintaining sperm viability, signaling, and motility, which are essential for fertilization. Sperm are highly susceptible to oxidative stress, as they are rich in sensitive polyunsaturated fatty acids (PUFA), and are unable to synthesize and repair many essential membrane constituents. Because of this, sperm cellular membranes are important targets of this process. Membrane Lipid Replacement (MLR) with glycerophospholipid mixtures (GPL) has been shown to ameliorate oxidative stress in cells, restore their cellular membranes, and prevent loss of function. Therefore, we tested the effects of MLR on sperm by tracking and monitoring GPL incorporation into their membrane systems and studying their effects on sperm motility and viability under different experimental conditions. Incubation of sperm with mixtures of exogenous, unoxidized GPL results in their incorporation into sperm membranes, as shown by the use of fluorescent dyes attached to GPL. The percent overall (total) sperm motility was increased from 52±2.5% to 68±1.34% after adding GPL to the incubation media, and overall sperm motility was recovered from 7±2% after H2O2 treatment to 58±2.5%)(n = 8, p<0.01) by the incorporation of GPL into sperm membranes. When sperm were exposed to H2O2, the mitochondrial inner membrane potential (MIMP), monitored using the MIMP tracker dye JC-1 in flow cytometry, diminished, whereas the addition of GPL prevented the decrease in MIMP. Confocal microscopy with Rhodamine-123 and JC-1 confirmed the mitochondrial localization of the dyes. We conclude that incubation of human sperm with glycerolphospholipids into the membranes of sperm improves sperm viability, motility, and resistance to oxidizing agents like H2O2. This suggests that human sperm might be useful to test innovative new treatments like MLR, since such treatments could improve fertility when it is adversely affected by increased oxidative stress.

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Lipid Regulation of Acrosome Exocytosis

Cited by 19 publications

References 68 publications

Boar seminal plasma exosomes maintain sperm function by infiltrating into the sperm membrane

Boar seminal plasma exosomes maintain sperm function by infiltrating into the sperm membrane

Progress in ciliary ion channel physiology

Incubation of human sperm with micelles made from glycerophospholipid mixtures increases sperm motility and resistance to oxidative stress

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