Lipid Rafts have Different Sizes Depending on Membrane Composition: A Time-resolved Fluorescence Resonance Energy Transfer Study

Almeida, Rodrigo F.M. de; Loura, Luís M. S.; Marques, Lino; Prieto, Manuel

doi:10.1016/j.jmb.2004.12.026

Cited by 273 publications

(276 citation statements)

References 51 publications

(276 reference statements)

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“…NBD-diC n PE probes have been used extensively as model phospholipids in the studies of thermodynamics and kinetics of interaction with lipid bilayers and other hydrophobic aggregates, such as serum lipoproteins. [17][18][19] Used as FRET donors, NBD-diC n PE probes form, together with acceptor rhodamine B PE derivatives, a very well-known Förster pair for the studies of membrane organization [20][21][22][23][24] as well as quantification of lipid mixing and exchange. 25 Alternatively, NBD probes can be used as acceptors to chromophores including diphenylhexatriene 26,27 or trans-parinaric acid, 28 or even as donors and acceptors simultaneously in homo-FRET studies.…”

Section: Introductionmentioning

confidence: 99%

“…25 Alternatively, NBD probes can be used as acceptors to chromophores including diphenylhexatriene 26,27 or trans-parinaric acid, 28 or even as donors and acceptors simultaneously in homo-FRET studies. 20 Several fluorescence spectroscopic and microscopic studies have also indicated that, contrary to the vast majority of membrane fluorescent probes, doubly saturated long-chained NBD-diC n PE partitions favourably to liquid ordered (lo) phases in systems displaying lo/liquid disordered phase coexistence, 22,24,29,30 although the phase preference depends both on the probe acyl chain length and the composition of the underlying lipid mixture. 17,31 Despite their wide use in membrane biophysics, the behaviour of NBD-diC n PE probes has so far lacked a characterization using MD.…”

Section: Introductionmentioning

confidence: 99%

Behaviour of NBD-head group labelled phosphatidylethanolamines in POPC bilayers: a molecular dynamics study

Filipe

Santos

Ramalho

et al. 2015

Phys. Chem. Chem. Phys.

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A complete homologous series of fluorescent phosphatidylethanolamines (diC n PE), labelled at the head group with a 7-nitrobenz-2-oxa-1,3-diazo-4-yl(NBD) fluorophore and inserted in 1-palmitoyl, 2-oleoyl-snglycero-3-phosphocholine (POPC) bilayers, was studied using atomistic molecular dynamics simulations. The longer-chained derivatives of NBD-diC n PE, with n = 14, 16, and 18, are commercially available, and widely used as fluorescent membrane probes. Properties such as location of atomic groups and acyl chain order parameters of both POPC and NBD-diC n PE, fluorophore orientation and hydrogen bonding, membrane electrostatic potential and lateral diffusion were calculated for all derivatives in the series. Most of these probes induce local disordering of POPC acyl chains, which is on the whole counterbalanced by ordering resulting from binding of sodium ions to lipid carbonyl/glycerol oxygen atoms. An exception is found for NBD-diC 16 PE, which displays optimal matching with POPC acyl chain length and induces a slight local ordering of phospholipid acyl chains. Compared to previously studied fatty amines, acyl chain-labelled phosphatidylcholines, and sterols bearing the same fluorescent tag, the chromophore in NBD-diC n PE locates in a similar region of the membrane (near the glycerol backbone/carbonyl region) but adopts a different orientation (with the NO 2 group facing the interior of the bilayer). This modification leads to an inverted orientation of the P-N axis in the labelled lipid, which affects the interface properties, such as the membrane electrostatic potential and hydrogen bonding to lipid head group atoms. The implications of this study for the interpretation of the photophysical properties of NBD-diC n PE (complex fluorescence emission kinetics, differences with other NBD lipid probes) are discussed.

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“…It has long been recognized that energy transfer between fluorescent membrane probes is eminently suited to the detection and study of coexisting membrane phase domains [19], and a number of groups have adapted FRET experiments to this end during the last decade, or so [e.g., 4,[20][21][22]. Indeed, Loura, de Almeida, Fedorov and Prieto have published several excellent studies [13,[23][24][25][26][27] based on time-resolved measurements of transfer efficiency, ) (t E , and Silvius and Nabi have recently produced a comprehensive review of FRET-based studies of membrane microdomains [28].…”

Section: Discussionmentioning

confidence: 99%

“…And as long as all relevant experimental parameters (e.g., spectrophotometer settings and global probe concentrations) are kept fixed throughout a given experiment, variations in coexisting membrane phases, it may first be noted that (i) on the timescale of typical excited state lifetimes (≤ 10 -8 s) there is little diffusive motion of membrane probes [11]; and that (ii) the characteristic spatial scale, R d , of many membrane phase domains is much larger than the donor-acceptor Förster distance, R 0 , of even the most efficient energy transfer probe pairs (i.e., R 0 ~ 5 nm). For both these reasons, the fraction of overall energy transfer that occurs across domain boundaries should be minimal 2 and the observed (i.e., global average) donor-excited acceptor fluorescence may be approximated as the sum of individual contributions 2 In order to allow for the study of "nanoscopic" membrane domains, which have Rd on the order of 100 nm or smaller [12,13], SP-FRET experiments can simply employ less efficient donor-acceptor pairs (i.e., probe combinations with reduced spectral overlap) in order to ensure that R0 << Rd.…”

Section: Modeling Sp-fret In a Regime Of Coexisting Phasesmentioning

confidence: 99%

Steady-state probe-partitioning fluorescence resonance energy transfer: A simple and robust tool for the study of membrane phase behavior

Buboltz

2007

Phys. Rev. E

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“…In phospholipid bilayers, the hydrophobic mismatch between the components in binary mixtures leads to different types of phase diagrams (Mabrey and Sturtevant 1976;de Almeida et al 2005). For PCs, a difference of six carbons in the acyl chain length in the mixture of symmetric dilauroyl-PC/distearoyl-PC produced a rather flattened fluid-gel coexistence line (Mabrey and Sturtevant 1976;Risbo et al 1995).…”

Section: Ceramide Mixturesmentioning

confidence: 99%

The many faces (and phases) of ceramide and sphingomyelin II – binary mixtures

Fanani

Maggio

2017

Biophys Rev

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A rather widespread idea on the functional importance of sphingolipids in cell membranes refers to the occurrence of ordered domains enriched in sphingomyelin and ceramide that are largely assumed to exist irrespective of the type of N-acyl chain in the sphingolipid. Ceramides and sphingomyelins are the simplest kind of two-chained sphingolipids and show a variety of species, depending on the fatty acyl chain length, hydroxylation, and unsaturation. Abundant evidences have shown that variations of the N-acyl chain length in ceramides and sphingomyelins markedly affect their phase state, interfacial elasticity, surface topography, electrostatics, and miscibility, and that even the usually conceived Bcondensed^sphingolipids and many of their mixtures may exhibit liquid-like expanded states. Their lateral miscibility properties are subtlety regulated by those chemical differences. Even between ceramides with different acyl chain length, their partial miscibility is responsible for a rich twodimensional structural variety that impacts on the membrane properties at the mesoscale level. In this review, we will discuss the miscibility properties of ceramide, sphingomyelin, and glycosphingolipids that differ in their N-acyl or oligosaccharide chains. This work is a second part that accompanies a previous overview of the properties of membranes formed by pure ceramides or sphingomyelins, which is also included in this Special Issue.

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Lipid Rafts have Different Sizes Depending on Membrane Composition: A Time-resolved Fluorescence Resonance Energy Transfer Study

Cited by 273 publications

References 51 publications

Behaviour of NBD-head group labelled phosphatidylethanolamines in POPC bilayers: a molecular dynamics study

Steady-state probe-partitioning fluorescence resonance energy transfer: A simple and robust tool for the study of membrane phase behavior

The many faces (and phases) of ceramide and sphingomyelin II – binary mixtures

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