Lipid Peroxidation in Ischemia-Reperfusion Oxidative Injury of the Graft Preserved in Celsior and University of Wisconsin Solutions on a Pig Pancreas Transplantation Model

García-Gil, Francisco A.; Gonzalvo, Elena; Garcia-Garcia, J.J.; Calleja, Carlos David Albendea; Güemes, Antonio; Tomé-Zelaya, E.; Fuentes, Laura; Santa-Clotilde, E.; Aso, J.; Bejarano, C.; Garrido, Nicolás; García, Claudia; Vilar‐Gomez, Eduardo; Sanchez, Mayra

doi:10.1016/j.transproceed.2006.08.030

Cited by 14 publications

(6 citation statements)

References 12 publications

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“…LPO occurs during reperfusion of the pancreatic graft with an extraordinary intensity (36,38). This is consistent with our UW data, where LPO after 30 min of graft reperfusion was increased when compared with the respective basal and cold ischemia levels.…”

Section: Discussionsupporting

confidence: 91%

“…IRI is the result of a complex sequence of pathologic events that also generates free radicals with consequent damage to lipids, proteins, DNA, and cell membranes (35Y37). LPO, one of the most deleterious effects of free radicals during cell damage (36,38), causes impairment of membrane functioning, including falls in membrane potential and increased permeability to ions such as H + and, Ca 2+ (and eventual rupture leading to release of cell and organelle contents, such as lysosomal hydrolytic enzymes). These alterations are characteristic effects of the organ static hypothermic preservation (39,40).…”

Section: Discussionmentioning

confidence: 99%

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Evaluation of Institut Georges Lopez-1 Preservation Solution in Pig Pancreas Transplantation

et al. 2014

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

Evaluation of Institut Georges Lopez-1 Preservation Solution in Pig Pancreas Transplantation

et al. 2014

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“…Initial hypoglycemia with hyperinsulinemia is typical in PTs with venous drainage of the graft to the systemic circulation; it is mainly attributed to the loss of first‐pass hepatic insulin clearance [39]. Early elevation of amylase and lipase in the blood is a consequence, at least partially, of the preservation and surgical manipulation of the pancreas [40, 41]. Although there were no statistically significant differences in the serum lipase peaks, melatonin reduced this enzyme level by 40% compared with that in untreated control animals.…”

Section: Discussionmentioning

confidence: 99%

Melatonin prolongs graft survival of pancreas allotransplants in pigs

García-Gil

Calleja

Escartín

et al. 2011

Journal of Pineal Research

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: Oxidative stress is involved in ischemia‐reperfusion injury and allograft rejection after transplantation. We studied two well‐known antioxidants, melatonin and ascorbic acid (AA), in relation to the survival of a pancreas transplantation model without immunosuppression. Forty‐eight Landrace pigs were divided into three groups (n = 16 each; eight donors and eight recipients) that received melatonin, AA, or no antioxidant therapy (controls). Melatonin and AA were administered (10 mg/kg body weight) intravenously to donors and recipients during surgery and on postoperative days 1–7. The molecules were also added (5 mm) to a University of Wisconsin preservation solution during organ cold storage. Melatonin significantly delayed acute rejection and prolonged allograft survival (25.1 ± 7.7 days) compared with the controls (8.1 ± 0.8 days, P = 0.013) and the AA group (9.4 ± 1.6 days, P = 0.049). Melatonin reduced indicators of oxidative stress, malondialdehyde, and 4‐hydroxyalkenals, in pancreatic samples collected during procurement, cold ischemia, and reperfusion. Melatonin also reduced serum pig‐major acute‐phase protein/inter‐α‐trypsin inhibitor heavy chain 4 (pMAP/ITIH4) in the early post‐transplantation period. AA only partially reduced oxidative damage 30 min postreperfusion and failed to prevent pMAP/ITIH4 elevations. These findings suggested that melatonin may be a useful therapeutic tool for organ transplantation.

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“…During the process of ischemic tissue restoring to aerobic metabolism, free radicals might be generated via oxidizing various cellular components [ 81 ]. Garci-Gil et al have shown that LPO is the major contributor to cause ischemia-reperfusion oxidative injury on a pig pancreas transplantation model [ 82 ]. Another study reported that reperfusion of ischemic cardiac tissue enhanced the concentration of LPO product 4-HNE, contributing to the declines in mitochondrial function [ 83 ].…”

Section: Ferroptosis In Cvdsmentioning

confidence: 99%

Ferroptosis: a cell death connecting oxidative stress, inflammation and cardiovascular diseases

et al. 2021

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Ferroptosis, a recently identified and iron-dependent cell death, differs from other cell death such as apoptosis, necroptosis, pyroptosis, and autophagy-dependent cell death. This form of cell death does not exhibit typical morphological and biochemical characteristics, including cell shrinkage, mitochondrial fragmentation, nuclear condensation. The dysfunction of lipid peroxide clearance, the presence of redox-active iron as well as oxidation of polyunsaturated fatty acid (PUFA)-containing phospholipids are three essential features of ferroptosis. Iron metabolism and lipid peroxidation signaling are increasingly recognized as central mediators of ferroptosis. Ferroptosis plays an important role in the regulation of oxidative stress and inflammatory responses. Accumulating evidence suggests that ferroptosis is implicated in a variety of cardiovascular diseases such as atherosclerosis, stroke, ischemia-reperfusion injury, and heart failure, indicating that targeting ferroptosis will present a novel therapeutic approach against cardiovascular diseases. Here, we provide an overview of the features, process, function, and mechanisms of ferroptosis, and its increasingly connected relevance to oxidative stress, inflammation, and cardiovascular diseases.

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Lipid Peroxidation in Ischemia-Reperfusion Oxidative Injury of the Graft Preserved in Celsior and University of Wisconsin Solutions on a Pig Pancreas Transplantation Model

Cited by 14 publications

References 12 publications

Evaluation of Institut Georges Lopez-1 Preservation Solution in Pig Pancreas Transplantation

Evaluation of Institut Georges Lopez-1 Preservation Solution in Pig Pancreas Transplantation

Melatonin prolongs graft survival of pancreas allotransplants in pigs

Ferroptosis: a cell death connecting oxidative stress, inflammation and cardiovascular diseases

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