Lipid peroxidation in cardiac mitochondrial fraction of rats exposed to different supplementation with polyunsaturated fatty acids

Ketsa, O. V.; Shmarakov, Igor O.; Марченко, М. М.

doi:10.1134/s1990750816030057

Cited by 2 publications

(4 citation statements)

References 12 publications

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“…For the analysis of individual fatty acids used standard drugs from Sigma. ω-3 PUFA was administered per os at a daily dose of 120 mg/kg body weight of animals, because at this dose exhibit antioxidant and membrane-protective effects [13].…”

Section: Methodsmentioning

confidence: 99%

“…3). Obviously, the previous introduction of ω-3 PUFA promotes their incorporation into the membrane structure of cells, which makes them resistant to free radical destruction of macromolecules [13]. On the other hand, the bioregulatory role of ω-3 PUFAs on mitochondrial function may be manifested through eicosanoids, which are synthesized from ω-3 PUFAs.…”

Section: Fig 3 the Rate Of Formation Of Superoxide Anion Radical In T...mentioning

confidence: 99%

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Proteins oxidative modification and antioxidant enzymes activity in the liver mitochondria of rats under laser irradiation and administration of ω-3 polyunsaturated fatty acids

Ketsa¹,

Zelinska²,

Марченко³

2022

Ukr. Biochem. J.

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The effect of laser irradiation of rats combined with omega-3 polyunsaturated fatty acids (ω-3 PUFA) administration on the proteins oxidative modification and superoxide dismutase and catalase activity in the mitochondrial fraction of the liver was investigated. Animals were irradiated with a 650 nm laser diode in the abdomen daily for 4 min at the distance of 10 cm from the skin surface. ω-3 PUFA were administered per os at a daily dose of 120 mg/kg of the body weight. Fatty acids in the fish oil were identified by gas chromatography. Animals were divided into five groups (12 animals in each group): I -intact rats (control); II -rats exposed to the daily laser irradiation for 7 or 14 days ; III -rats that received ω-3 PUFA two hours after irradiation; IV -rats that received ω-3 PUFA two hours before irradiation; V -rats that received ω-3 PUFA for 7 days before irradiation. The mitochondrial fraction of rat liver was obtained by differential centrifugation. The increase in the content of protein carbonyl derivatives and a decrease of protein thiol groups in the liver mitochondrial fraction were detected after seven-day laser irradiation of rats. As the duration of irradiation increases, superoxide dismutase and catalase activity was decreased, indicating a depletion of mitochondrial antioxidant reserves. No antioxidant effect was observed when ω-3 PUFA were administrated after laser irradiation and a slight antioxidant effect was shown when ω-3 PUFA were administrated two hours before irradiation. Preliminary seven-day administration ω-3 PUFA before laser irradiation was the most effective, as it reduced the level of protein carbonyl derivatives and O 2•--generation, increased proteins SH-groups content and antioxidant enzymes activity. K e y w o r d s: SH-groups, carbonyl derivatives, superoxide radical, laser irradiation, ω-3 polyunsaturated fatty acids, liver mitochondria, superoxide dismutase and catalase activity.

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Section: Methodsmentioning

confidence: 99%

Section: Fig 3 the Rate Of Formation Of Superoxide Anion Radical In T...mentioning

confidence: 99%

Proteins oxidative modification and antioxidant enzymes activity in the liver mitochondria of rats under laser irradiation and administration of ω-3 polyunsaturated fatty acids

Ketsa¹,

Zelinska²,

Марченко³

2022

Ukr. Biochem. J.

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“…Приріст АЛТ, який перевищує підвищення АСТ, характерніший для пошкодження печінки. Якщо ж показник АСТ підвищується більше, ніж АЛТ, -як правило, це свідчить про порушення клітин міокарда [6] Note. In the first (F 0 ) generation: * -P≤0.001 compared to rats consuming untreated transgenic soybean.…”

Section: результати й обговоренняunclassified

“…Ймовірно, що гербіцид «Раундап» як ксенобіотик біотрансформується системою цитохрому Р450 печінки у потенційно токсичні метаболіти, здатні провокувати пошкодження клітинних мембран [7]. Один із механізмів такого пошкодження -ініціація пероксидного окиснення ліпідів мембран ендоплазматичного ретикулума, мітохондрій, плазматичної мембрани клітин печінки [6]. Внаслідок пошкодження плазмолеми або цитолізу гепатоцитів АСТ і АЛТ потрапляють у кров, що виявляється збільшенням їх концентрації.…”

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Biochemical markers of the functional state of liver in blood serum of rats consuming glyphosate-resistant genetically modified soybean and herbicide “Roundup”

Чорна¹,

Dronic²,

Kulish³

2021

Bìol. Tvarin

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Тhe effect of glyphosate-resistant genetically modified soybean and herbicide “Roundup” on the enzymatic activity of aspartate aminotransferase (AST), alanine aminotransferase (ALT) and γ-glutamyltransferase (GGT), and on the content of middle mass molecules in rat serum has been investigated. The studies were made on the Wistar rats divided into five groups: 1st group — intact; 2nd group — 25% of rat’s ration was replaced by traditional soybean; 3rd group — the rats received feed containing genetically modified soybean not treated with the herbicide “Roundup”; 4th group — the rats received feed containing genetically modified soybean treated with the herbicide; 5th group — the rats received the herbicide “Roundup” with drinking water. After 42 days females of all groups were mated and continued to receive the same diet and herbicide with drinking water. In 22–24 days the next generation of rats was born. In the first and second generation of rats fed with the herbicide “Roundup” and transgenic soybean treated with this herbicide, the hyperenzymemia of ALT, AST and GGT in compared with control group was observed. At the same time, De Ritis Ratio wass reduced to values of 0.8 and 0.7 for the rats fed with soybean “Roundup Ready” and herbicide, respectively. It is shown that the level of middle mass molecules in blood serum of the first rat generation increases in 1.5 and 1.6 times in cases of feeding rats with genetically modified soybean treated with herbicide and herbicide only; the increase in the content of middle mass molecules is observed in the second generation too. The increase of middle mass molecules content in blood serum indicates a syndrome of endogenous intoxication.

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Lipid peroxidation in cardiac mitochondrial fraction of rats exposed to different supplementation with polyunsaturated fatty acids

Cited by 2 publications

References 12 publications

Proteins oxidative modification and antioxidant enzymes activity in the liver mitochondria of rats under laser irradiation and administration of ω-3 polyunsaturated fatty acids

Proteins oxidative modification and antioxidant enzymes activity in the liver mitochondria of rats under laser irradiation and administration of ω-3 polyunsaturated fatty acids

Biochemical markers of the functional state of liver in blood serum of rats consuming glyphosate-resistant genetically modified soybean and herbicide “Roundup”

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