Lipid mediators in marine diatoms

Orefice, Ida; Dato, Valeria Di; Sardo, Angela; Lauritano, Chiara; Romano, Giovanna

doi:10.1007/s10452-021-09932-8

Cited by 9 publications

(6 citation statements)

References 153 publications

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“…There are several examples of chemically mediated communication in unicellular organisms ( [40]), with some of them having a critical role in dormancy ( [41]). In diatoms, the most studied infochemicals related to intra-and inter-specific communication are compounds belonging to the oxylipin family [42], which include polyunsaturated aldehydes (PUAs) and linear oxygenated fatty acids (LOFAs), both derived from the oxygenation of fatty acids ( [43]). The first step of their synthesis derives from the oxidation of membrane lipids, through several enzymes among which a crucial role is played by lipoxygenases.…”

Section: Chemical Signaling Pathways Related To Oxylipin Productionmentioning

confidence: 99%

Gene expression during the formation of resting spores induced by nitrogen starvation in the marine diatom Chaetoceros socialis

Pelusi¹,

Ambrosino²,

Miralto³

et al. 2023

BMC Genomics

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Background Dormancy is widespread in both multicellular and unicellular organisms. Among diatoms, unicellular microalgae at the base of all aquatic food webs, several species produce dormant cells (spores or resting cells) that can withstand long periods of adverse environmental conditions. Results We present the first gene expression study during the process of spore formation induced by nitrogen depletion in the marine planktonic diatom Chaetoceros socialis. In this condition, genes related to photosynthesis and nitrate assimilation, including high-affinity nitrate transporters (NTRs), were downregulated. While the former result is a common reaction among diatoms under nitrogen stress, the latter seems to be exclusive of the spore-former C. socialis. The upregulation of catabolic pathways, such as tricarboxylic acid cycle, glyoxylate cycle and fatty acid beta-oxidation, suggests that this diatom could use lipids as a source of energy during the process of spore formation. Furthermore, the upregulation of a lipoxygenase and several aldehyde dehydrogenases (ALDHs) advocates the presence of oxylipin-mediated signaling, while the upregulation of genes involved in dormancy-related pathways conserved in other organisms (e.g. serine/threonine-protein kinases TOR and its inhibitor GATOR) provides interesting avenues for future explorations. Conclusions Our results demonstrate that the transition from an active growth phase to a resting one is characterized by marked metabolic changes and provides evidence for the presence of signaling pathways related to intercellular communication.

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Section: Chemical Signaling Pathways Related To Oxylipin Productionmentioning

confidence: 99%

Gene expression during the formation of resting spores induced by nitrogen starvation in the marine diatom Chaetoceros socialis

Pelusi¹,

Ambrosino²,

Miralto³

et al. 2023

BMC Genomics

View full text Add to dashboard Cite

show abstract

“…There are several examples of chemically mediated communication in unicellular organisms ( [39]), with some of them having a critical role in dormancy ( [40]). In diatoms, the most studied infochemicals related to intra-and inter-speci c communication are compounds belonging the oxylipin family, which include polyunsaturated aldehydes (PUAs) and linear oxygenated fatty acids (LOFAs), both derived from the oxygenation of fatty acids ( [41]). The production of LOFAs has been reported for C. socialis although the enzymatic pathway involved is still unknown ( [42]).…”

Section: Pathways Related To Oxylipin Productionmentioning

confidence: 99%

Gene expression during the formation of resting spores in the marine diatom Chaetoceros socialis

Pelusi

Ambrosino

Miralto

et al. 2022

Preprint

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BackgroundDormancy is widespread in both multicellular and unicellular organisms. Among diatoms, unicellular microalgae at the base of all aquatic food webs, several species produce dormant cells (spores or resting cells) that can withstand long periods of adverse environmental conditions. ResultsWe present the first gene expression study during the process of spore formation in the marine planktonic diatom Chaetoceros socialis. Spore formation was induced by nitrogen depletion. Genes related to photosynthesis and nitrate assimilation, including high-affinity nitrate transporters (NTRs), were downregulated. While the former result is a common reaction among diatoms under nitrogen stress, the latter seems to be exclusive of the spore-former C. socialis. The upregulation of catabolic pathways, such as tricarboxylic acid cycle, glyoxylate cycle and beta-oxidation, suggests that this diatom could use lipids as a source of energy during the process of spore formation. Furthermore, the upregulation of a lipoxygenase and several aldehyde dehydrogenases (ALDHs) advocates the presence of oxylipin-mediated signalling, while the upregulation of genes involved in dormancy-related pathways conserved in other organisms (e.g. serine/threonine-protein kinases TOR and its inhibitor GATOR) provides interesting avenues for future explorations. ConclusionsOur results demonstrate that the transition from an active growth phase to a resting one is characterized by marked metabolic changes and provide evidence for the presence of signalling pathways related to intercellular communication.

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“…The oxylipins are also byproducts generated from ROS reaction from polyunsaturated fatty acids (PUFAs), called lipid peroxidation (Améras et al, 2003; Jahn et al, 2008; Triantaphylidès et al, 2008). These compounds represent the best described signaling molecules in diatoms (Orefice et al, 2022; Ruocco et al, 2020). The oxylipins can influence other species abundance and fitness through the antipredator, antibacterial, infochemical and allelochemical functions of oxylipins (Meyer et al, 2018; Ruocco et al, 2020; Russo et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

Non-enzymatic oxylipin production in a mudflat microphytobenthic biofilm: evidence of a diatom response to light

Doose,

Oger,

Mas-Normand

et al. 2024

Preprint

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The microphytobenthos (MPB) is a diatom dominated microbial community of primary producers inhabiting the mudflat sediments. In one hand, the benthic diatoms display photo-protective strategies to face extreme light variations susceptible to generate cellular oxidative stress. In the other hand, oxidative stress induces the production of reactive oxygen species (ROS) that generate oxylipins, oxygenated metabolites of polyunsaturated fatty acids (PUFAs), which are among the known chemical mediators in diatoms. However, non-enzymatically generated oxylipins known as isoprostanoids or isofuranoids are poorly studied in diatoms. To better understand the roles of the latter in migrational MPB light response, we investigated the effect of different light irradiances corresponding to dark (D), low light (LL, 50 and 100 umol. photons. m-2. s-1PAR), medium light (ML, 250 umol. photons. m-2. s-1 PAR), high light (HL, 500, 750 and 1000 umol. photons. m-2. s-1PAR), on the isoprostanoids production by the biofilm's organisms. The PUFAs precursors of the varying oxylipins evidenced a diatoms response to light irradiance. Under 1000 PAR condition, the total amount of isoprotanoids increased, indicating an oxidative stress response. Isoprostanes (IsoPs) and prostaglandins (PGs) characterized the HL conditions and evidenced lipid peroxidation probably linked to the higher generation of ROS by the photosynthesis. On the contrary, the phytoprostanes (PhytoPs) characterized the LL and ML where the de-epoxidation state was low and ROS scavengers probably not overwhelmed. This first investigation of non-enzymatic oxylipin production by a microphytobenthic biofilm under different light irradiances highlighted the interest to explore their potential signaling roles related to MPB light responses.

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Lipid mediators in marine diatoms

Cited by 9 publications

References 153 publications

Gene expression during the formation of resting spores induced by nitrogen starvation in the marine diatom Chaetoceros socialis

Gene expression during the formation of resting spores induced by nitrogen starvation in the marine diatom Chaetoceros socialis

Gene expression during the formation of resting spores in the marine diatom Chaetoceros socialis

Non-enzymatic oxylipin production in a mudflat microphytobenthic biofilm: evidence of a diatom response to light

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