Lipid imaging in the zebra finch brain with secondary ion mass spectrometry

The application of mass spectrometry to imaging, or MS imaging (MSI), allows for the direct investigation of tissue sections to identify biological compounds and determine their spatial distribution. We present an approach to MSI that combines secondary ion mass spectrometry (SIMS) and MALDI MS for the imaging and analysis of rat spinal cord sections, thereby enhancing the chemical coverage obtained from an MSI experiment. The spinal cord is organized into discrete, anatomically defined areas that include motor and sensory networks composed of chemically diverse cells. The MSI data presented here reveal the spatial distribution of multiple phospholipids, proteins, and neuropeptides obtained within single, 20-μm sections of rat spinal cord. Analyte identities are initially determined by primary mass match and confirmed in follow-up experiments using LC MS/ MS from extracts of adjacent spinal cord sections. Additionally, a regional analysis of differentially localized signals serves to rapidly screen compounds of varying intensities across multiple spinal regions. These MSI analyses reveal new insights into the chemical architecture of the spinal cord and set the stage for future imaging studies of the chemical changes induced by pain, anesthesia, and drug tolerance.

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“…1R). These differences in localization based on the level of fatty acid unsaturation have been previously reported [36]. …”

Section: Resultssupporting

confidence: 67%

SIMS and MALDI MS imaging of the spinal cord

et al. 2008

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“…Some of the earliest studies described the presence of steroid hormone binding sites [26] and recently this description has advanced even to the level of small fatty acids [27]. Expression of several genes, including the immediate early gene zenk ( zif268, egr-1, ngfi-a, krox24 ) is activated by both the sound of birdsong [28] and the act of singing [29,30], but in different components of the song control system.…”

Section: The Genome and The Brainmentioning

confidence: 99%

Integrating Genomes, Brain and Behavior in the Study of Songbirds

2009

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Songbirds share some essential traits but are extraordinarily diverse, allowing comparative analyses aimed at identifying specific genotype–phenotype associations. This diversity encompasses traits like vocal communication and complex social behaviors that are of great interest to humans, but that are not well represented in other accessible research organisms. Many songbirds are readily observable in nature and thus afford unique insight into the links between environment and organism. The distinctive organization of the songbird brain will facilitate analysis of genomic links to brain and behavior. Access to the zebra finch genome sequence will, therefore, prompt new questions and provide the ability to answer those questions.

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“…Mass spectral images often contain 10 4 pixels and have been as large as 10 7 pixels (Amaya et al 2007), depending on the size of the sample and the spatial resolution. Since a mass spectrum is taken at each pixel location, dataset sizes can rapidly approach and even exceed several gigabytes.…”

Section: Image Acquisition and Data Analysismentioning

confidence: 99%

Chapter 13 Imaging of Cells and Tissues with Mass Spectrometry

Zimmerman

Monroe

Tucker

et al. 2008

Methods in Cell Biology

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Techniques that map the distribution of compounds in biological tissues can be invaluable in addressing a number of critical questions in biology and medicine. One of the newest methods, mass spectrometric imaging, has enabled investigation of spatial localization for a variety of compounds ranging from atomics to proteins. The ability of mass spectrometry to detect and differentiate a large number of unlabeled compounds makes the approach amenable to the study of complex biological tissues. This chapter focuses on recent advances in the instrumentation and sample preparation protocols that make mass spectrometric imaging of biological samples possible, including strategies for both tissue and single cell imaging using the following mass spectrometric ionization methods: matrix-assisted laser desorption/ionization, secondary ion, electrospray and desorption electrospray.

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Lipid imaging in the zebra finch brain with secondary ion mass spectrometry

Cited by 31 publications

References 39 publications

SIMS and MALDI MS imaging of the spinal cord

SIMS and MALDI MS imaging of the spinal cord

Integrating Genomes, Brain and Behavior in the Study of Songbirds

Chapter 13 Imaging of Cells and Tissues with Mass Spectrometry

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