Lipid/alginate nanoparticle-loaded in situ gelling system tailored for dexamethasone nasal delivery

Dukovski, Bisera Jurišić; Plantić, Ivana; Čunčić, Ivan; Krtalić, Iva; Juretić, Marina; Pepić, Ivan; Lovrić, Jasmina; Hafner, Anita

doi:10.1016/j.ijpharm.2017.05.065

Cited by 30 publications

(22 citation statements)

References 35 publications

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“…Simulated nasal fluid (SNF) was prepared as an aqueous solution containing NaCl (150.0 mM; Kemig, Croatia), KCl (40.0 mM; Kemig, Croatia), and CaCl 2 x2H 2 O (5.3 mM; Sigma-Aldrich, Germany) [10] and it was used for swelling and in vitro release studies. Hank's balanced salt solution (HBSS) with 5.3 mM Ca 2þ (HBSS-Ca 2þ ; pH 7.4) was prepared by dissolving KCl (5.4 mM), NaHCO 3 (4.2 mM), NaCl (136.9 mM), D-glucose monohydrate (5.6 mM; all purchased from Kemig, Croatia), KH 2 PO 4 (0.4 mM; Kemika, Croatia), Na 2 HPO 4 x2H 2 O (0.3 mM; Fluka Chemie AG, Switzerland), and CaCl 2 x2H 2 O (5.3 mM; Sigma-Aldrich, Germany) in double-distilled water [11] and was used for cell viability and in vitro permeability studies. All other reagents were of analytical grade and purchased from Kemika, Croatia, and Sigma-Aldrich, Germany.…”

Section: Methodsmentioning

confidence: 99%

“…Dex-loaded lipid/alginate NPs were prepared according to the procedure described previously. [11] Briefly, lecithin, DDAB, and Dex were dissolved in 96% ethanol at the concentrations of 25, 2.5, and 5 mg mL À1 , respectively. Alginate was solubilized in distilled water at a concentration of 10 mg mL À1 and the aliquot of alginate solution (0.5 mL) was further diluted with distilled water to a volume of 23 mL.…”

Section: Preparation Of Lipid/alginate Npsmentioning

confidence: 99%

“…Ten milligrams of spray-dried NP-loaded pectin microspheres were homogeneously dispersed in 1.33 mL of distilled water to reach the same Dex concentration as in Dex-loaded NP suspension with 5 mg mL À1 of pectin in the continuous phase [11] . The dispersion was further diluted with HBSS-Ca 2þ (pH 7.4) 2, 4, 8, and 16 times.…”

Section: Cell Viability Studymentioning

confidence: 99%

“…In vitro permeability studies using Caco-2 cell monolayer were performed as described previously. [11] Caco-2 cells were seeded onto the polycarbonate 12-well Transwell inserts (0.4 lm mean pore size and 1.12 cm 2 surface area, Corning Costar Inc., Corning, NY, USA) at a density of 2.5 Â 10 4 cells per well and grown for 21 days. The cells were cultured with the cell culture medium volume of 0.5 mL in the apical and 1.5 mL in the basolateral compartment.…”

Section: In Vitro Permeability Through Epithelial Model Barriermentioning

confidence: 99%

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Spray-dried nanoparticle-loaded pectin microspheres for dexamethasone nasal delivery

et al. 2019

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In this study, we present the development of an innovative dry powder dexamethasone (Dex) nasal delivery system comprising Dex-loaded lipid/alginate nanoparticles incorporated within pectin microspheres (Dex/NPs-loaded pectin microspheres; DNM). DNM microspheres were characterized by the mean diameter of 2.76 ± 0.10 mm, zeta-potential of -36.2 ± 1.1 mV, and drug loading of 3.3 ± 0.3%. The morphology study revealed irregular microsphere surface forming external voids. In contact with simulated nasal fluid, DNM microspheres demonstrated desirable property of moderate swelling and ensured stronger mucoadhesion compared with conventional Dex-loaded pectin microspheres. The strategy of Dex incorporation within the lipid/alginate NPs resulted in prolonged Dex release in relation to Dex being directly entrapped within the conventional pectin microspheres. DNM microspheres showed excellent biocompatibility and rendered Dex permeation across the selected epithelial cell model similar to that of Dex solution. In conclusion, balanced biopharmaceutical properties of the proposed nasal Dex delivery system provides the potential for prolonged contact time with nasal mucosa, prolonged therapeutic effect, and improved patient compliance.

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Section: Methodsmentioning

confidence: 99%

Section: Preparation Of Lipid/alginate Npsmentioning

confidence: 99%

Section: Cell Viability Studymentioning

confidence: 99%

Section: In Vitro Permeability Through Epithelial Model Barriermentioning

confidence: 99%

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Spray-dried nanoparticle-loaded pectin microspheres for dexamethasone nasal delivery

et al. 2019

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“…Dukovski and colleagues [ 53 ] developed an in situ gelling formulation for corticosteroid delivery in the treatment of chronic rhinosinusitis with nasal polyps. In particular, dexamethasone (DEX)-loaded lipid/ALG nanoparticles (NPs) were dispersed in a PEC solution that could be easily sprayed at the site of inflammation by an appropriate nasal delivery device; the ability of PEC to form gels upon contact with the Ca 2+ ions present in the nasal mucosa is responsible for the sol–gel transition.…”

Section: Nasal Routementioning

confidence: 99%

Recent Advances in the Development of In Situ Gelling Drug Delivery Systems for Non-Parenteral Administration Routes

et al. 2020

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In situ gelling drug delivery systems have gained enormous attention over the last decade. They are in a sol-state before administration, and they are capable of forming gels in response to different endogenous stimuli, such as temperature increase, pH change and the presence of ions. Such systems can be administered through different routes, to achieve local or systemic drug delivery and can also be successfully used as vehicles for drug-loaded nano- and microparticles. Natural, synthetic and/or semi-synthetic polymers with in situ gelling behavior can be used alone, or in combination, for the preparation of such systems; the association with mucoadhesive polymers is highly desirable in order to further prolong the residence time at the site of action/absorption. In situ gelling systems include also solid polymeric formulations, generally obtained by freeze-drying, which, after contact with biological fluids, undergo a fast hydration with the formation of a gel able to release the drug loaded in a controlled manner. This review provides an overview of the in situ gelling drug delivery systems developed in the last 10 years for non-parenteral administration routes, such as ocular, nasal, buccal, gastrointestinal, vaginal and intravesical ones, with a special focus on formulation composition, polymer gelation mechanism and in vitro release studies.

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An Up‐to‐Date Review on Alginate Nanoparticles and Nanofibers for Biomedical and Pharmaceutical Applications

Dodero

Alberti

Gaggero

et al. 2021

Adv Materials Inter

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along with other polysaccharides (e.g., celluloses, chitosan, etc.), is indeed considered an extremely promising material in the viewpoint of circular economy and in substituting petroleum-derived polymers. [3] Among others, one of the reasons for alginate success is its unique capability to bind different cations leading to stable and tailor-made hydrogels. [4] Owing to its biocompatibility, biodegradability, and nontoxicity, in the past decades alginate has been vastly explored for drug and gene delivery, tissue engineering, and wound healing applications. [5] In this sense, the recent nanotechnological advances have allowed the fabrication and exploitation of alginate-based nanostructured materials with completely new capabilities. Thereby, it is not surprising that nowadays a broad variety of alginate-based nanomaterials with various shapes, sizes, and compositions are prepared via different approaches, including controlled gelation, electrospinning and electrospraying, self-assembly, phase-separation, and micro fluidics., All these nanostructures hold the potential to interact with living organisms much more efficiently with respect to bulk systems, hence leading to specific functionalities at the same time avoiding the occurrence of toxicity issues. [6][7][8] Despite the use of alginate-based nanomaterials has been reviewed in the past, the focus has been commonly pointed to their generic properties and applications. Conversely, this review attempts to provide a more specific and comprehensive summary limited to the most recent advances in the fabrication and use of two of the most common and promising alginatebased nanomaterials, namely nanoparticles (NPs) and electrospun nanofibers (NFs), with a focus on biomedical and pharmaceutical applications.

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Lipid/alginate nanoparticle-loaded in situ gelling system tailored for dexamethasone nasal delivery

Cited by 30 publications

References 35 publications

Spray-dried nanoparticle-loaded pectin microspheres for dexamethasone nasal delivery

Spray-dried nanoparticle-loaded pectin microspheres for dexamethasone nasal delivery

Recent Advances in the Development of In Situ Gelling Drug Delivery Systems for Non-Parenteral Administration Routes

An Up‐to‐Date Review on Alginate Nanoparticles and Nanofibers for Biomedical and Pharmaceutical Applications

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