Lipid A analog CRX-527 conjugated to synthetic peptides enhances vaccination efficacy and tumor control

Tondini, Elena; Reintjens, Niels R. M.; Castello, Giulia; Arakelian, Tsolère; Isendoorn, Marjolein M.E.; Camps, Marcel; Vree, Jana; Marel, Gijs A. van der; Filippov, Dmitri V.; Codée, Jeroen D. C.; Ossendorp, Ferry

doi:10.1038/s41541-022-00484-y

Cited by 6 publications

(2 citation statements)

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“…The inclusion of the ASO4 adjuvant in this vaccine has been shown to evoke a more robust immune response in vaccinated people (98). Other synthetic lipid adjuvants such as 3M-052, GLA-SE, CRX-527, Ono-4007, OM-174, and DT-5461 have also been developed and applied for this purpose (99)(100)(101)(102)(103). Despite their somewhat limited clinical efficacy on their own, incorporating lipids as adjuvants in cancer therapy has promising potential, especially for tumors of low immunogenicity.…”

Section: Lipids As Adjuvantsmentioning

confidence: 99%

Lipid metabolism in tumor immunology and immunotherapy

et al. 2023

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Lipids are a diverse class of biomolecules that have been implicated in cancer pathophysiology and in an array of immune responses, making them potential targets for improving immune responsiveness. Lipid and lipid oxidation also can affect tumor progression and response to treatment. Although their importance in cellular functions and their potential as cancer biomarkers have been explored, lipids have yet to be extensively investigated as a possible form of cancer therapy. This review explores the role of lipids in cancer pathophysiology and describes how further understanding of these macromolecules could prompt novel treatments for cancer.

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Section: Lipids As Adjuvantsmentioning

confidence: 99%

Lipid metabolism in tumor immunology and immunotherapy

et al. 2023

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“…In this context, GP5-derived peptides have been studied as neutralization epitopes, particularly linear peptides [ 32 ]. Peptides can bind to MHC and activate the immune system [ 33 ]; they are also capable of activating dendritic cells (DCs), promoting DC maturation and T-cell activation in vitro, stimulating DC migration, and the priming of functional T-cells in vivo [ 34 ].…”

Section: Introductionmentioning

confidence: 99%

Glycoprotein 5-Derived Peptides Induce a Protective T-Cell Response in Swine against the Porcine Reproductive and Respiratory Syndrome Virus

Calderon-Rico,

Bravo-Patiño,

Mendieta

et al. 2023

Viruses

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We analyzed the T-cell responses induced by lineal epitopes of glycoprotein 5 (GP5) from PRRSV to explore the role of this protein in the immunological protection mediated by T-cells. The GP5 peptides were conjugated with a carrier protein for primary immunization and booster doses. Twenty-one-day-old pigs were allocated into four groups (seven pigs per group): control (PBS), vehicle (carrier), PTC1, and PTC2. Cytokine levels were measured at 2 days post-immunization (DPI) from serum samples. Cytotoxic T-lymphocytes (CTLs, CD8+) from peripheral blood were quantified via flow cytometry at 42 DPI. The cytotoxicity was evaluated by co-culturing primed lymphocytes with PRRSV derived from an infectious clone. The PTC2 peptide increased the serum concentrations of pro-inflammatory cytokines (i.e., TNF-α, IL-1β, IL-8) and cytokines that activate the adaptive cellular immunity associated with T-lymphocytes (i.e., IL-4, IL-6, IL-10, and IL-12). The concentration of CTLs (CD8+) was significantly higher in groups immunized with the peptides, which suggests a proliferative response in this cell population. Primed CTLs from immunized pigs showed cytolytic activity in PRRSV-infected cells in vitro. PTC1 and PTC2 peptides induced a protective T-cell-mediated response in pigs immunized against PRRSV, due to the presence of T epitopes in their sequences.

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Analysis of carbohydrates and glycoconjugates by matrix‐assisted laser desorption/ionization mass spectrometry: An update for 2021–2022

Harvey

2024

Mass Spectrometry Reviews

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The use of matrix‐assisted laser desorption/ionization (MALDI) mass spectrometry for the analysis of carbohydrates and glycoconjugates is a well‐established technique and this review is the 12th update of the original article published in 1999 and brings coverage of the literature to the end of 2022. As with previous review, this review also includes a few papers that describe methods appropriate to analysis by MALDI, such as sample preparation, even though the ionization method is not MALDI. The review follows the same format as previous reviews. It is divided into three sections: (1) general aspects such as theory of the MALDI process, matrices, derivatization, MALDI imaging, fragmentation, quantification and the use of computer software for structural identification. (2) Applications to various structural types such as oligo‐ and polysaccharides, glycoproteins, glycolipids, glycosides and biopharmaceuticals, and (3) other general areas such as medicine, industrial processes, natural products and glycan synthesis where MALDI is extensively used. Much of the material relating to applications is presented in tabular form. MALDI is still an ideal technique for carbohydrate analysis, particularly in its ability to produce single ions from each analyte and advancements in the technique and range of applications show little sign of diminishing.

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Lipid A analog CRX-527 conjugated to synthetic peptides enhances vaccination efficacy and tumor control

Cited by 6 publications

References 50 publications

Lipid metabolism in tumor immunology and immunotherapy

Lipid metabolism in tumor immunology and immunotherapy

Glycoprotein 5-Derived Peptides Induce a Protective T-Cell Response in Swine against the Porcine Reproductive and Respiratory Syndrome Virus

Analysis of carbohydrates and glycoconjugates by matrix‐assisted laser desorption/ionization mass spectrometry: An update for 2021–2022

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