Lipase immobilization on magnetic microspheres via spacer arms: Effect of steric hindrance on the activity

Zhang, Dong‐Hao; Li, Yaqiong; Peng, Li-Juan; Chen, Na

doi:10.1007/s12257-013-0495-x

Cited by 21 publications

(9 citation statements)

References 25 publications

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“…This effect could be due to enzyme overcrowding on the particle surface, which can result in lower enzymatic activity due to steric hindrance. 50,51 Although (to the best of our knowledge) this behavior has not been observed for urease micromotors before, Yang et al do observe a similar trend for their MOFtors, where an increase in catalase loading resulted in less generation of the O 2 product. 4 Thus, the optimal concentration of immobilized urease for self-propulsion of Urhex motors is ∼60 mg/mL.…”

Section: Resultssupporting

confidence: 67%

Enzyme Purification Improves the Enzyme Loading, Self-Propulsion, and Endurance Performance of Micromotors

et al. 2022

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Enzyme-powered micro-and nanomotors make use of biocatalysis to self-propel in aqueous media and hold immense promise for active and targeted drug delivery. Most (if not all) of these micro-and nanomotors described to date are fabricated using a commercially available enzyme, despite claims that some commercial preparations may not have a sufficiently high degree of purity for downstream applications. In this study, the purity of a commercial urease, an enzyme frequently used to power the motion of micro-and nanomotors, was evaluated and found to be impure. After separating the hexameric urease from the protein impurities by size-exclusion chromatography, the hexameric urease was subsequently characterized and used to functionalize hollow silica microcapsules. Micromotors loaded with purified urease were found to be 2.5 times more motile than the same micromotors loaded with unpurified urease, reaching average speeds of 5.5 μm/s. After comparing a number of parameters, such as enzyme distribution, protein loading, and motor reusability, between micromotors functionalized with purified vs unpurified urease, it was concluded that protein purification was essential for optimal performance of the enzyme-powered micromotor.

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Section: Resultssupporting

confidence: 67%

Enzyme Purification Improves the Enzyme Loading, Self-Propulsion, and Endurance Performance of Micromotors

et al. 2022

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“…This might be ascribed to the following reasons: The conformation of the enzyme molecule immobilized on the porous microspheres was somewhat changed compared to the free enzyme (Mannen et al, 2001;Liu et al, 2013;Secundo, 2013). In addition, the immobilization led to an increase in the steric hindrance between the enzyme and the substrate (Ying et al, 2002;Zhang et al, 2012;Rodrigues et al, 2013;Jahangiri et al, 2014;Zhang et al, 2014) which caused a decrease in the activity of the immobilized enzyme. However, the unique structure of the porous microspheres could impart a good microenvironment to the immobilized enzyme molecules, reduce the influence of external adverse factors on the enzyme molecules, and thereby improve the thermal stability (Han et al, 2015;Han et al, 2016).…”

Section: Immobilization Of Rate-limited Enzyme (Ips) Of the Pathway Fmentioning

confidence: 99%

Efficient Multi-Enzymes Immobilized on Porous Microspheres for Producing Inositol From Starch

Han

Zhou

You

2020

Front. Bioeng. Biotechnol.

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In vitro synthetic enzymatic biosystem is considered to be the next generation of biomanufacturing platform. This biosystem contains multiple enzymes for the implementation of complicated biotransformatiom. However, the hard-to-reuse and instability of multiple enzymes limit the utilization of this biosystem in industrial process. Multi-enzyme immobilization might be a feasible alternative to address these problems. Herein, porous microspheres are used as carriers to co-immobilize multiple enzymes for producing inositol from starch. At first, all the enzymes (i.e., α-glucan phosphorylase aGP, phosphoglucose mutase PGM, inositol 1-phosphate synthase IPS, and inositol monophosphatase IMP) for converting starch to inositol were immobilized on porous microspheres individually to check the effect of immobilization, then all the enzymes are co-immobilized on porous microspheres. Compared to reaction system containing all the individual immobilized enzymes, the reaction system containing the co-immobilized enzymes exhibit ∼3.5 fold of reaction rate on producing inositol from starch. This reaction rate is comparable to that by free enzyme mixture. And the co-immobilized multi-enzyme system show higher thermal stability and recovery stability than free enzyme mixture. After 7 batches, the immobilized enzymes retain 45.6% relative yield, while the free enzyme mixture only retain 13.3% relative yield after 3 batches. Coimmobilization of multiple enzymes on porous microspheres for biomanufacturing would shed light on the application of in vitro synthetic enzymatic biosystem in industrial scale.

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“…It was speculated that 3-chloropropyltriethoxysilane (CPS), as a spacer, could reduce steric hindrance, so that more enzymes could bind to the surface of the supporting materials. 36 Secondly, the covalent attachment also played an important role in the performance. Thirdly, magnetic mesoporous silicon was deposited on the surface of graphene oxide, which provided more surface area and more chemical functional groups that could absorb PPL.…”

Section: Ppl Immobilizationmentioning

confidence: 99%

“…40 Compared to nonimmobilized PPL, immobilized PPL has advantages in terms of stability at different pH values, which was similar to previous reports. [35][36][37] Fig. 7 TGA curves of GO and CPS/GO-Fe 3 O 4 @MCM-41.…”

Section: Ppl Immobilizationmentioning

confidence: 99%

Graphene oxide-based Fe₃O₄ nanoparticles as a novel scaffold for the immobilization of porcine pancreatic lipase

et al. 2015

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Lipase immobilization on magnetic microspheres via spacer arms: Effect of steric hindrance on the activity

Cited by 21 publications

References 25 publications

Enzyme Purification Improves the Enzyme Loading, Self-Propulsion, and Endurance Performance of Micromotors

Enzyme Purification Improves the Enzyme Loading, Self-Propulsion, and Endurance Performance of Micromotors

Efficient Multi-Enzymes Immobilized on Porous Microspheres for Producing Inositol From Starch

Graphene oxide-based Fe₃O₄ nanoparticles as a novel scaffold for the immobilization of porcine pancreatic lipase

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Lipase immobilization on magnetic microspheres via spacer arms: Effect of steric hindrance on the activity

Cited by 21 publications

References 25 publications

Enzyme Purification Improves the Enzyme Loading, Self-Propulsion, and Endurance Performance of Micromotors

Enzyme Purification Improves the Enzyme Loading, Self-Propulsion, and Endurance Performance of Micromotors

Efficient Multi-Enzymes Immobilized on Porous Microspheres for Producing Inositol From Starch

Graphene oxide-based Fe3O4 nanoparticles as a novel scaffold for the immobilization of porcine pancreatic lipase

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Graphene oxide-based Fe₃O₄ nanoparticles as a novel scaffold for the immobilization of porcine pancreatic lipase