Linearity of Calibration Curves for Analytical Methods: A Review of Criteria for Assessment of Method Reliability

Moosavi, Seyed Mojtaba; Ghassabian, Sussan

doi:10.5772/intechopen.72932

Cited by 98 publications

(109 citation statements)

References 18 publications

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“…The bioassay presented a broad linear response from 1 pg mL −1 to 10 µg mL −1 and curve equation of eCL factor = (15.562 ± 0.328) − (0.789 ± 0.042) × Ln(C haptoglobin ). It should be highlighted that the wide range of reference haptoglobin concentrations are prone to heteroscedasticity within semi-logarithmic scale, thus leading to inaccuracy of the calibration [36]. However, herein, the presented correlation coefficient (R 2 = 0.98) depicts sufficient linearity and minimal statistical error deviation of the sampling points (in the range of 0.4%–5.8%).…”

Section: Resultsmentioning

confidence: 81%

Gold Nanoparticle Size-Dependent Enhanced Chemiluminescence for Ultra-Sensitive Haptoglobin Biomarker Detection

Nirala

Shtenberg

2019

Biomolecules

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Bovine mastitis (BM) is a frequent disease in the dairy industry that causes staggering economical losses due to decreased milk production and increased health care costs. Traditionally, BM detection depends on the efficacy and reliability of analytical techniques that measure somatic cell counts (SCC), detect pathogens, and reveal inflammatory status. Herein, we demonstrate the detection of bovine haptoglobin, a well-documented acute phase protein for evaluating BM clinical status, by utilizing hemoglobin-binding capacity within luminol chemiluminescence (CL) system. The resulting haptoglobin–hemoglobin complex reduces the CL signal proportionally to inherent haptoglobin concentrations. Different sizes of cross-linked gold nanoparticles (GNPs) were examined for enhanced CL (eCL) signal amplification, presenting over 30-fold emitted radiation enhancement for optimized size within real milk samples with respect to nanoparticle-free assay. The eCL values were proportionally related to nanoparticle size and content, influenced by SCC and pathogen type (e.g., Escherichia coli and coagulase-negative staphylococci). The optimized bioassay showed a broad linear response (1 pg mL−1–10 µg mL−1) and minute detection limit of 0.19 pg mL−1, while presenting quantitative performance in agreement with commercial ELISA kit. Finally, the resulting optimized eCL concept offers an efficient label-free detection of haptoglobin biomarker, offering means to diagnose the severity of the associated diseases.

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Section: Resultsmentioning

confidence: 81%

Gold Nanoparticle Size-Dependent Enhanced Chemiluminescence for Ultra-Sensitive Haptoglobin Biomarker Detection

Nirala

Shtenberg

2019

Biomolecules

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“…Furthermore, the spike-and-recovery test was applied to assess accuracy, which was calculated by adding a known amount of analyte to different volumes of pure standard cortisol solution. Only one value did not correspond to the required results: the inter-assay CV of Anas was just above the 15% reference point, from which a non-optimal inter-assay performance can be concluded [63][64][65]. However, in summary, the validation tests confirmed that it is possible to detect corticosterone concentrations in feathers of Anser and Anas with an acceptable repeatability and reliability by using ELISA.…”

Section: Discussionmentioning

confidence: 91%

Validation of an Alternative Feather Sampling Method to Measure Corticosterone

Voit

Merle

Baumgärtner

et al. 2020

Animals

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The most common feather sampling method for feather corticosterone measurement is by plucking the feathers from the bird’s skin. This procedure performed on living, restrained birds is qualified as an animal experiment according to German/European legislation, which has to be applied for from the competent authorities. The Directive 2010/63/EU requires the full implementation of the 3-R Principle of Russel and Burch in animal experiments, which means not only to replace the use of animals, but also to reduce the number of animals used and to refine procedures whenever possible. In response to this issue, the aim of this study was to validate an alternative, less invasive sampling method by cutting feathers close to the skin in comparison to the gold standard of plucking them. For this proof-of-principle study, a conventional poultry husbandry with trial groups of geese (Anser anser domesticus) and ducks (Anas sterilis) was selected. All birds were kept under the same living conditions to standardize the influencing factors regarding husbandry, and thus, their stress levels. Feather samples were collected between the shoulders from 46 geese and 51 ducks, both by cutting as well as by plucking, directly after slaughter for meat production. Feather corticosterone levels were measured with Enzyme-Linked Immunosorbent Assay (ELISA). Results were compared using Bland–Altman plots and concordance correlation coefficients (CCC). It could be seen that concordance between corticosterone levels in cut and plucked feathers was rather poor: 0.38 for Anser, and 0.57 for Anas. However, comparing the mean corticosterone values in pg/mm of each species with their respective standard deviations, the differences between the methods were negligible. As the results showed that the differences between the individuals were markedly greater than the differences between the methods, the determination of corticosterone levels in cut feathers is valid compared to using plucked feathers. The validation tests of ELISA showed only acceptable repeatability and reliability. Hence, the results should be verified in further studies. In conclusion, it is recommended for future research to use cut instead of plucked feathers for corticosterone measurement.

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“…The acquired concentration curve of A VI confers two messages. First, since A VI is not proportional to C VI , as shown in Figure 2 , traditional methods are not applicable 49 for the quantitative analysis of Cr(VI) in water based on its detected SERS signal. Second, the effective concentration range of SERS detection is roughly between 0.1 and 2 mg/L.…”

Section: Resultsmentioning

confidence: 99%

Speciation Analysis of Cr(VI) and Cr(III) in Water with Surface-Enhanced Raman Spectroscopy

Dvoynenko

Chen

et al. 2021

ACS Omega

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Identifying and quantifying chromium in water are important for the protection of precious water resources from chromium pollution. Standard methods however are unable to easily distinguish toxic hexavalent chromium, Cr(VI), from innocuous trivalent chromium, Cr(III), are time-consuming, or require large sample quantity. We show in this report that Cr(VI) and Cr(III) in water can be differentiated based on their distinct spectral features of surface-enhanced Raman scattering (SERS). Their SERS signals exhibit different pH dependences: the SERS features of Cr(VI) and Cr(III) are most prominent at pH values of 10 and 5.5, respectively. The obtained limit of detection of Cr(VI) in water is below 0.1 mg/L. Both concentration curves of their SERS signals show Langmuir sorption isotherm behavior. A procedure was developed to quantify Cr(VI) concentration based on the direct retrieval or addition method with an error of 10%. Finally, the SERS detection of Cr(VI) is shown to be insensitive to co-present Cr(III). The developed SERS procedure offers potential to monitor toxic chromium in fields.

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Linearity of Calibration Curves for Analytical Methods: A Review of Criteria for Assessment of Method Reliability

Cited by 98 publications

References 18 publications

Gold Nanoparticle Size-Dependent Enhanced Chemiluminescence for Ultra-Sensitive Haptoglobin Biomarker Detection

Gold Nanoparticle Size-Dependent Enhanced Chemiluminescence for Ultra-Sensitive Haptoglobin Biomarker Detection

Validation of an Alternative Feather Sampling Method to Measure Corticosterone

Speciation Analysis of Cr(VI) and Cr(III) in Water with Surface-Enhanced Raman Spectroscopy

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