LINC01006 facilitates cell proliferation, migration and invasion in prostate cancer through targeting miR-34a-5p to up-regulate DAAM1

Abstract

Background: Prostate cancer (PCa) is a kind of malignancy occurring in the prostate gland. Substantial researches have proved the major role of long noncoding RNAs (lncRNAs) in PCa. However, the role of long intergenic non-protein coding RNA 1006 (LINC01006) in PCa has not been investigated yet.Methods: RT-qPCR was used to examine the expression levels of LINC01006 and its downstream targets. The function of LINC01006 in PCa was tested by in vitro and in vivo assays. With application of RNA pull down, RNA immunoprecipitation (RIP) and luciferase reporter assays, the interaction among LINC01006, miR-34a-5p and disheveled associated activator of morphogenesis 1 (DAAM1) were verified.Results: LINC01006 expression presented high in PCa cell lines. LINC01006 silencing suppressed cell proliferative, migratory, invasive capacities while accelerated apoptotic rate. Besides, LINC01006 knockdown also suppressed tumor growth and metastasis in vivo. Furthermore, miR-34a-5p, a tumor suppressor in PCa, was sponged by LINC01006. Moreover, DAAM1 was targeted by miR-34a-5p and promoted PCa progression. More intriguingly, rescue assays suggested that the inhibitory effect of LINC01006 knockdown on PCa development was offset by DAAM1 overexpression.Conclusions: LINC01006 promoted PCa progression by sponging miR-34a-5p to up-regulate DAAM1, providing a novel target for PCa therapy.

SNHG15 facilitated malignant behaviors of oral squamous cell carcinoma through targeting miR‐188‐5p/DAAM1

“…DAAM1 was discovered to have oncogenic function in ovarian cancer via inhibiting the expression of miR‐208a‐5p expression 32 . In addition, DAAM1 could facilitate prostate cancer cell proliferation, migration, and invasion via LINC01006/miR‐34a‐5p axis 33 . In this study, DAAM1 was determined to be the target gene of miR‐188‐5p and its expression was detected to be high in OSCC cells.…”

Mesenchymal Stem Cell-derived Extracellular Vesicles Transmitting MicroRNA-34a-5p Suppress Tumorigenesis of Colorectal Cancer Through c-MYC/DNMT3a/PTEN Axis

“…A RT-qPCR tested miR-34a-5p expression in HCT-116 cells after miR-34a-5p inhibition; B MTT tested viability of HCT-116 cells after miR-34a-5p inhibition; C flow cytometry tested apoptosis of HCT-116 cells after miR-34a-5p inhibition; D Transwell assay tested migration of HCT-116 cells after miR-34a-5p inhibition; E Transwell assay tested invasion of HCT-116 cells after miR-34a-5p inhibition; F RT-qPCR tested Ecadherin mRNA expression in HCT-116 cells after miR-34a-5p inhibition; G RT-qPCR tested N-cadherin mRNA expression in HCT-116 cells after miR-34a-5p inhibition; H RT-qPCR tested Vimentin mRNA expression in HCT-116 cells after miR-34a-5p inhibition; I RT-qPCR tested Snail mRNA expression in HCT-116 cells after miR-34a-5p inhibition; the data were expressed as mean ± standard deviation of three independent experiments. * P < 0.05 compared with the NC-inhibitor group morphogenesis 1, thus to accelerate cancer development [53]. As to liver cancer, miR-34a-5p exerts anti-proliferative, anti-migratory, and anti-invasive effects on cancer cells through targeted regulation of Yin and Yang 1 [54].…”

Long non‑coding RNA LINC01006 exhibits oncogenic properties in cervical cancer by functioning as a molecular sponge for microRNA‑28‑5p and increasing PAK2 expression

“…LINc01006 is upregulated in prostate (20) and pancreatic (21) cancers and plays pro-oncogenic roles. By contrast, this lncRNA is weakly expressed in gastric cancer and exhibits a notable association with age, tumor location, tumor size and venous invasion (22).…”

“…Previous studies have confirmed the abnormal expression of the long intergenic non-protein-coding RNA 1006 (LINc01006) in prostate (20), pancreatic (21) and gastric (22) cancers. However, whether LINc01006 plays important roles in cervical cancer remains unclear.…”