Lignin plays a key role in determining biomass recalcitrance in forage grasses

Oliveira, Dyoni Matias de; Mota, Thatiane Rodrigues; Grandis, Adriana; Morais, Gutierrez Rodriguês de; Lucas, Rosymar Coutinho de; Polizeli, M. L. T. M.; Marchiosi, Rogério; Buckeridge, Marcos Silveira; Ferrarese-Filho, Osvaldo; Santos, Wanderley Dantas dos

doi:10.1016/j.renene.2019.10.020

Cited by 41 publications

(21 citation statements)

References 54 publications

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“…The lignin content found in this work are consistent with previous studies and indicates that P. maximum has similar percent composition of lignin with those reported for sugarcane bagasse, about 27.79% (Lima et al 2014;Oliveira et al 2019a). Therefore, lignin amount present in P. maximum does not represent a possible inconvenience (in terms of biomass deconstruction and modification) when compared to sugarcane bagasse (Table 1).…”

Section: Biomass Chemical Compositionsupporting

confidence: 91%

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Climate change affects cell wall structure and hydrolytic performance of a tropical forage grass as an energy crop

Freitas

Khatri

Contin

et al. 2020

Preprint

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Forage grasses, such as Panicum maximum, are important alternatives of lignocellulosic biomass for bioethanol production. Thus, this study investigates whether future climate conditions could influence P. maximum cell wall structure and hydrolytic performance. A combined temperature-free air controlled and a free-air carbon dioxide enrichment (Trop-T-FACE) facility was used to investigated the isolated and combined effect of elevated atmospheric CO2 concentration (eC) (600 μmol.mol-1) and elevated temperature (eT) by 2˚C more than the ambient temperature, on cell wall composition, cellulose crystallinity, accessibility, and hydrolysis yields. The elevated temperature treatments (eT and eT+eC) exhibited the most pronounced effects. Warming reduced starch content and crystallinity index (CI) of cellulose while increased cellulose content. The fluorescent protein-tagged carbohydrate-binding modules analysis demonstrated that warming led to improvement in the total cellulose surface exposure/accessibility in eT and eT+eC by 181% and 132%, respectively. Consequently, glucan conversion yields were improved by 7.07 and 5.37%, showing that warming led to lower recalcitrance in P. maximum biomass, which positively affect its use in biorefineries. Therefore, this work provides important information from an ecological and economic point of view, and might assist in the selection of tropical forage grasses efficiently adapted to climate changes with positive effect on bioenergy production.

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Section: Biomass Chemical Compositionsupporting

confidence: 91%

“…The cell wall bound phenolics analysis was done as described elsewhere (Oliveira et al 2019a). Quantification of cell wall-bound phenolics was accomplished by a HPLC system (Shimadzu® Liquid Chromatograph, Tokyo, Japan).…”

Section: Profile Of Cell Wall-bound Phenolicsmentioning

confidence: 99%

Climate change affects cell wall structure and hydrolytic performance of a tropical forage grass as an energy crop

Freitas

Khatri

Contin

et al. 2020

Preprint

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“…Arabinofuranosyl residues (Ara f ) are α‐(1,2)‐ or α‐(1,3)‐linked to the xylan chain assembling the arabinoxylan (AX), which may be further esterified with FA (and p CA at lower levels) at the O ‐5 position of Ara f residues. Feruloylation of AX may act to cross‐link AX chains to each other or to lignins, resulting in a highly recalcitrant lignin−hydroxycinnamate−carbohydrate complex (de Oliveira et al, 2015; Grabber, Ralph, & Hatfield, 2000; Hatfield et al, 2017; Oliveira et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

“…The corresponding loading plots show the contribution of each wavelength to the separation of the samples on each principal component (PC). The loading plots for PC1 and PC2 (Figure 1c) revealed two large peaks at wavelengths corresponding to C O and C O C stretching assigned to cell wall polysaccharides(950-1,100 cm −1 ) and C C stretching and aromatic skeletal vibrations assigned to lignin (1,510-1,630 cm −1 )(Oliveira et al, 2020).…”

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confidence: 99%

Cell wall remodeling under salt stress: Insights into changes in polysaccharides, feruloylation, lignification, and phenolic metabolism in maize

Oliveira

Mota

Salatta

et al. 2020

Plant Cell & Environment

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Although cell wall polymers play important roles in the tolerance of plants to abiotic stress, the effects of salinity on cell wall composition and metabolism in grasses remain largely unexplored. Here, we conducted an in-depth study of changes in cell wall composition and phenolic metabolism induced upon salinity in maize seedlings and plants. Cell wall characterization revealed that salt stress modulated the deposition of cellulose, matrix polysaccharides and lignin in seedling roots, plant roots and stems. The extraction and analysis of arabinoxylans by size-exclusion chromatography, 2D-NMR spectroscopy and carbohydrate gel electrophoresis showed a reduction of arabinoxylan content in salt-stressed roots. Saponification and mild acid hydrolysis revealed that salinity also reduced the feruloylation of arabinoxylans in roots of seedlings and plants. Determination of lignin content and composition by nitrobenzene oxidation and 2D-NMR confirmed the increased incorporation of syringyl units in lignin of maize roots. Salt stress also induced the expression of genes and the activity of enzymes enrolled in phenylpropanoid biosynthesis. The UHPLC-MS-based metabolite profiling confirmed the modulation of phenolic profiling by salinity and the accumulation of ferulate and its derivatives 3-and 4-O-feruloyl

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“…However, the complex network of cross‐links in grass cell walls can inhibit the digestion by preventing enzymatic access to the biomass, hindering the cell‐wall deconstruction and the release of fermentable sugars for ethanol production. Therefore, decreasing the cross‐links content of cell walls is considered a promising strategy for increasing digestibility (Mnich et al ., 2020; Oliveira et al ., 2020a). Understanding the plant cell wall architecture, the acylation patterns and its biosynthesis is an efficient way to accelerate the development of technologies for cellulosic ethanol production.…”

Section: Introductionmentioning

confidence: 99%

Suppression of a BAHD acyltransferase decreases p‐coumaroyl on arabinoxylan and improves biomass digestibility in the model grass Setaria viridis

et al. 2020

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SummaryGrass cell walls have hydroxycinnamic acids attached to arabinosyl residues of arabinoxylan (AX), and certain BAHD acyltransferases are involved in their addition. In this study, we characterized one of these BAHD genes in the cell wall of the model grass Setaria viridis. RNAi silenced lines of S. viridis (SvBAHD05) presented a decrease of up to 42% of ester‐linked p‐coumarate (pCA) and 50% of pCA‐arabinofuranosyl, across three generations. Biomass from SvBAHD05 silenced plants exhibited up to 32% increase in biomass saccharification after acid pre‐treatment, with no change in total lignin. Molecular dynamics simulations suggested that SvBAHD05 is a p‐coumaroyl coenzyme A transferase (PAT) mainly involved in the addition of pCA to the arabinofuranosyl residues of AX in Setaria. Thus, our results provide evidence of p‐coumaroylation of AX promoted by SvBAHD05 acyltransferase in the cell wall of the model grass S. viridis. Furthermore, SvBAHD05 is a promising biotechnological target to engineer crops for improved biomass digestibility for biofuels, biorefineries and animal feeding.

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Lignin plays a key role in determining biomass recalcitrance in forage grasses

Cited by 41 publications

References 54 publications

Climate change affects cell wall structure and hydrolytic performance of a tropical forage grass as an energy crop

Climate change affects cell wall structure and hydrolytic performance of a tropical forage grass as an energy crop

Cell wall remodeling under salt stress: Insights into changes in polysaccharides, feruloylation, lignification, and phenolic metabolism in maize

Suppression of a BAHD acyltransferase decreases p‐coumaroyl on arabinoxylan and improves biomass digestibility in the model grass Setaria viridis

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