Lignin peroxidase is involved in the biobleaching of manganese-less oxygen-delignified hardwood kraft pulp by white-rot fungi in the solid-fermentation system

Machii, Yoshiharu

doi:10.1016/j.femsle.2004.02.019

Cited by 12 publications

(9 citation statements)

References 10 publications

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“…It has been suggested that MnP, LiP, and laccase produced extracellularly by white rot fungi are involved in the oxidative breakdown of lignin [38][39][40][41][42][43][44]. Therefore, these enzyme activities were determined during treatment of genistein with P. sordida YK-624 ( Fig.…”

Section: Treatment Of Genistein With White Rot Fungusmentioning

confidence: 99%

Removal of estrogenic activity of endocrine-disrupting genistein by ligninolytic enzymes from white rot fungi

Tamagawa

Hirai

Kawai

et al. 2005

FEMS Microbiology Letters

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Endocrine-disrupting genistein was treated with the white rot fungus Phanerochaete sordida YK-624 under ligninolytic condition with low-nitrogen and high-carbon culture medium. Genistein decreased by 93% after 4 days of treatment and the activities of ligninolytic enzymes, manganese peroxidase (MnP) and laccase, were detected during treatment, thus suggesting that the disappearance of genistein is related to ligninolytic enzymes produced extracellularly by white rot fungi. Therefore, genistein was treated with MnP, laccase, and the laccase-mediator system with 1-hydroxybenzotriazole (HBT) as a mediator. HPLC analysis demonstrated that genistein disappeared almost completely in the reaction mixture after 4 h of treatment with either MnP, laccase, or the laccase-HBT system. Using the yeast two-hybrid assay system, it was also confirmed that three enzymatic treatments completely removed the estrogenic activity of genistein after 4h. These results strongly suggest that ligninolytic enzymes are effective in removing the estrogenic activity of genistein.

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Section: Treatment Of Genistein With White Rot Fungusmentioning

confidence: 99%

Removal of estrogenic activity of endocrine-disrupting genistein by ligninolytic enzymes from white rot fungi

Tamagawa

Hirai

Kawai

et al. 2005

FEMS Microbiology Letters

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“…The white‐rot fungus Phanerochaete sordida YK‐624, which was isolated from rotted wood, exhibits greater ligninolytic activity and selectivity among beech woods than either Phanerochaete chrysosporium or Trametes versicolor (Hirai et al ., ). In a previous study, we demonstrated that P. sordida YK‐624 produces MnP (Hirai et al ., , ) and LiP (Sugiura et al ., ; Machii et al ., ; Hirai et al ., ) as ligninolytic enzymes.…”

Section: Introductionmentioning

confidence: 98%

Improvement of ligninolytic properties in the hyper lignin-degrading fungus Phanerochaete sordida YK-624 using a novel gene promoter

Sugiura

Mori

Kamei

et al. 2012

FEMS Microbiol Lett

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We identified a highly expressed protein (BUNA2) by two‐dimensional gel electrophoresis from the hyper lignin‐degrading fungus Phanerochaete sordida YK‐624 under wood‐rotting conditions. Partial amino acid sequences of BUNA2 were determined by LC‐MS/MS analysis, and BUNA2 gene (bee2) and promoter region were PCR‐cloned and sequenced. The bee2 promoter was used to drive the expression of the manganese peroxidase gene (mnp4) in P. sordida YK‐624. Eighteen mnp4‐expressing clones were obtained, with most showing higher ligninolytic activity and selectivity than wild‐type YK‐624. Examination of the ligninolytic properties of the most effective lignin‐degrading transformant, BM‐65, cultured on wood meal revealed that this strain exhibited higher lignin degradation and MnP activities than those of wild type. Transcriptional analysis confirmed the increased expression of recombinant mnp4 in the transformant. These results indicate that use of the bee2 promoter to drive the expression of ligninolytic enzymes may be an effective approach for improving the lignin‐degrading properties of white‐rot fungi.

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“…The same unique nonspecific mechanisms that give these fungi the ability to degrade lignin also allow them to degrade a wide range of pollutants, thus conferring them a number of advantages not associated with other bioremediation systems (1). This has opened new prospects for the development of biotechnological processes aimed at the degradation of xenobiotic compounds (2), effluent decolorization (3,4), and pulp biobleaching (5,6).…”

Section: Introductionmentioning

confidence: 99%

Laccase‐Mediated Remazol Brilliant Blue R Decolorization in a Fixed‐Bed Bioreactor

Palmieri

Giardina

Sannia

2005

Biotechnology Progress

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A crude laccase mixture preparation from Pleurotus ostreatus cultures supplemented with copper and ferulic acid was used to decolorize the anthraquinonic dye Remazol Brilliant Blue R (RBBR). Performance of this enzymatic system was tested, and a maximum of 70% decolorization was achievable under optimal conditions. The crude preparation was immobilized by entrapment in copper alginate beads attaining 65% yield of laccase activity. Stability of the immobilized laccases was remarkably increased in comparison with that of the free enzyme preparation. Efficiency of the immobilized system was evaluated during stepwise dye additions in batch operations. Under the best conditions, 70% RBBR decolorization was achieved even after 20 cycles, although decolorization time exponentially increased after the 10th cycle. Different fixed-bed bioreactors were prepared and analyzed in continuous decolorization processes. The best performance was obtained by decreasing the amount of enzyme loaded and by improving laccase retention using chitosan-coated alginate beads.

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Lignin peroxidase is involved in the biobleaching of manganese-less oxygen-delignified hardwood kraft pulp by white-rot fungi in the solid-fermentation system

Cited by 12 publications

References 10 publications

Removal of estrogenic activity of endocrine-disrupting genistein by ligninolytic enzymes from white rot fungi

Removal of estrogenic activity of endocrine-disrupting genistein by ligninolytic enzymes from white rot fungi

Improvement of ligninolytic properties in the hyper lignin-degrading fungus Phanerochaete sordida YK-624 using a novel gene promoter

Laccase‐Mediated Remazol Brilliant Blue R Decolorization in a Fixed‐Bed Bioreactor

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