Lignin and flavonoid content increases in Hypericum perforatum cell wall after Agrobacterium tumefaciens co-cultivation

Singh, Rupesh Kumar; Hou, Weina; Marslin, Gregory; Dias, Acp; Franklin, Gregory

doi:10.1055/s-0034-1394589

Cited by 7 publications

(7 citation statements)

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“…Analysis of the soluble phenolic fraction revealed an enormous increase in xanthone concentration and the emergence of many xanthones in H. perforatum cells after Agrobacterium co-cultivation was observed, while flavonoid content remained unaffected (Franklin et al, 2009a ). Recently, we studied changes in H. perforatum cell wall fractions and cell wall bound phenolic compounds in response to A. tumefaciens elicitation (Singh et al, 2014 ). This study revealed that lignin content was significantly increased in H. perforatum cell walls after A. tumefaciens elicitation (0.085–0.24 mg/mg dry weight cell wall) implying that H. perforatum reinforced its cell wall as a protective measure against A. tumefaciens infection.…”

Section: Dna Delivery Into H Perforatum Plant Celmentioning

confidence: 99%

“…content was also significantly higher in the cell walls of elicited cells compared to controls. Hence, in addition to PAL, 4CL, and BPS (Franklin et al, 2009a ), chalcone synthase (CHS) is also upregulated after elicitation (Singh et al, 2014 ). While those xanthones produced in response to A. tumefaciens elicitation were incorporated into the soluble phenolic fraction, flavonoids were actually incorporated into the cell wall.…”

Section: Dna Delivery Into H Perforatum Plant Celmentioning

confidence: 99%

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A Perspective on Hypericum perforatum Genetic Transformation

2016

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Hypericum perforatum (St John's wort) is a reservoir of diverse classes of biologically active and high value secondary metabolites, which captured the interest of both researchers and the pharmaceutical industry alike. Several studies and clinical trials have shown that H. perforatum extracts possess an astounding array of pharmacological properties. These properties include antidepressant, anti-inflammatory, antiviral, anti-cancer, and antibacterial activities; and are largely attributed to the naphtodianthrones and xanthones found in the genus. Hence, improving their production via genetic manipulation is an important strategy. In spite of the presence of contemporary genome editing tools, genetic improvement of this genus remains challenging without robust transformation methods in place. In the recent past, we found that H. perforatum remains recalcitrant to Agrobacterium tumefaciens mediated transformation partly due to the induction of plant defense responses coming into play. However, H. perforatum transformation is possible via a non-biological method, biolistic bombardment. Some research groups have observed the induction of hairy roots in H. perforatum after Agrobacterium rhizogenes co-cultivation. In this review, we aim at updating the available methods for regeneration and transformation of H. perforatum. In addition, we also propose a brief perspective on certain novel strategies to improve transformation efficiency in order to meet the demands of the pharmaceutical industry via metabolic engineering.

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Section: Dna Delivery Into H Perforatum Plant Celmentioning

confidence: 99%

Section: Dna Delivery Into H Perforatum Plant Celmentioning

confidence: 99%

A Perspective on Hypericum perforatum Genetic Transformation

2016

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“…In addition, the contents of lignin and flavonoids (quercetin, quercetrin etc.) were also significantly increased in the cell wall fraction of phenolics after elicitation with A. tumefaciens . Similarly, improvement of phenolic, flavonol, flavanol and xanthone concentrations in response to A. tumefaciens and A. rhizogenes treatment was observed in cell suspension cultures .…”

Section: Manipulation Of Hypericum Perforatum Secondary Metabolism VImentioning

confidence: 62%

“…were also significantly increased in the cell wall fraction of phenolics after elicitation with A. tumefaciens. [72] Similarly, improvement of phenolic, flavonol, flavanol and xanthone concentrations in response to A. tumefaciens and A. rhizogenes treatment was observed in cell suspension cultures. [73] However, A. rhizogenes was less effective in the induction of secondary metabolism compared to A. tumefaciens.…”

Section: Fungi and Extracts From Fungal Myceliamentioning

confidence: 78%

“…[70] These authors also reported that treating H. perforatum shoot cultures with a S. maltophilia culture filtrate induced only pseudohypericin production. [70] Analysis of the methanolic extract of cell suspension cultures treated with A. tumefaciens revealed a 12-fold increase in the total xanthone concentration and the emergence of [41] Iron nano-oxide Hypericin and hyperforin [41] Chitin Phenolics, flavonols, flavanols, anthocyanins, hypericin and pseudohypericin [66] Pectin Phenolics, flavonols, flavanols, anthocyanins, hypericin and pseudohypericin [66] Dextran Phenolics, flavonols, flavanols, anthocyanins, hypericin and pseudohypericin [66] Phytophthora cinnamoni fungal cell wall extract None [39] Colletotrichum gloeosporioides mycelium extract Xanthones and flavonoids [35] Aspergillus flavus mycelium extract Phenolic compounds, flavanols, flavonols, anthocyanins and hypericins [62] Fusarium oxysporum Phenolics, flavonoids, anthocyanins, hypericin and pseudohypericin [63] Phoma exigua Phenolics, flavonoids, anthocyanins, hypericin and pseudohypericin [63] Botrytis cinerea Phenolics, flavonoids, anthocyanins, hypericin and pseudohypericin [63] JA Hypericins [39] JA Phenolic compounds, flavanols, flavonols, hypericin and pseudohypericin [38] MeJA Flavones [35] MeJA Flavonoids [8] SA Xanthones [85] SA None [35] SA None [39] SA Hypericins, pseudohypericins [86] SA Flavonoids [8] MeJA + C. gloeosporioides Xanthones [35] SA + C. gloeosporioides Xanthones [35] Agrobacterium tumefaciens Xanthones [71] A. tumefaciens Lignin and flavanoids [72] A. tumefaciens Phenolics, flavonols, flavanols and xanthones [73] A. rhizogenes Phenolics, flavonols, flavanols and xanthones [73] Callus SA Hypericins, pseudohypericins [86] Root MeJA Hypericin [84] IBA Hypericin [84] Acetic acid Xanthones…”

Section: Fungi and Extracts From Fungal Myceliamentioning

confidence: 99%

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Elicitation as a tool to improve the profiles of high-value secondary metabolites and pharmacological properties of Hypericum perforatum

Shakya

Marslin

Karthik

et al. 2017

Journal of Pharmacy and Pharmacology

121

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Objectives In this review, we aim at updating the available information on the improvement of the Hypericum perforatum L. (Hypericaceae) phytochemical profile and pharmacological properties via elicitation. Key findings Hypericum perforatum seedlings, shoots, roots, calli and cell suspension cultures were treated with diverse elicitors to induce the formation of secondary metabolites. The extracts of the elicitor-treated plant material containing naphthodianthrones, phloroglucinols, xanthones, flavonoids and other new compounds were quantitatively analysed and tested for their bioactivities. While hypericins were mainly produced in H. perforatum cultures containing dark nodules, namely shoots and seedlings, other classes of compounds such as xanthones, phloroglucinols and flavonoids were formed in all types of cultures. The extracts obtained from elicitor-treated samples generally possessed better bioactivities compared to the extract of control biomass. Summary Although elicitation is an excellent tool for the production of valuable secondary metabolites in H. perforatum cell and tissue cultures, its exploitation is still in its infancy mainly due to the lack of reproducibility and difficulties in scaling up biomass production.

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