Light Scattering Studies of Hydration and Structural Transformations of Lysozyme

Abstract

We have applied the method of dynamic light scattering to analyse the lysozymeethanol interaction. For low ethanol concentration (below 4.3% (v/v)) no chemical denaturation process is observed. When the ethanol concentration grows above the triggering concentration the hydrodynamic radius of lysozyme increases, indicating the structural changes within the protein molecule. The observed structural modications are attributed to dehydration and preliminary tertiary structure modication of the protein molecule.

Denaturation and aggregation of lysozyme in water-ethanol solution.

“…With the increase in the ethanol concentration, up to about 30% (v/v), the specific volume increases up to the value of 0.726 cm 3 · g -1 . These data are in a good correlation with our observations of the changes of lysozyme hydrodynamic radius, R H (Szymańska & Ślósarek, 2012). According to the data from dynamic light scattering R H remains constant and equals (1.856±0.023) nm when the ethanol concentration increases up to about 4.3% (v/v) and then starts to increase with a growing amount of alcohol.…”

The Effect of Protein PEGylation on Physical Stability in Liquid Formulation

“…R h of Lyz was determined to be 23 Å. This is larger than 18–19 Å often reported in the literature . These values are almost always reported at pH 4.5 in solutions containing at least 0.1 M salt.…”

“…This is larger than 18-19Å often reported in the literature. 67,68 These values are almost always reported at pH 4.5 in solutions containing at least 0.1 M salt. Esposito et al 69 found that R h was only 6Å at pH 3 in the presence of 4 mM NaCl.…”

Nanofibrillar cellulose hydrogels and reconstructed hydrogels as matrices for controlled drug release

“…* MZ, NAD, KETO and BSA formulations were also prepared with 1 % of PEG6000 and 0.3 % of trehalose for freeze-drying. (Avdeef, et al 2000;Sheng, et al 2006;Singhai, et al 1996;Zhou, et al 2005) NAD* 309 8.3 (25 °C), 8.3 (pH 7) 9.28 + 1.7 (Avdeef and Berger 2001;Zhou, et al 2005) MZ* 171 10.5 (25 °C), 10.5 (pH 7) 2.38 ø 2 (Kim, et al 2012;Wu and Fassihi 2005;Zhou, et al 2005) BSA* 6.65x10 4 40 4.6 -4.8 -~ 7 1 (Sigma-Aldrich; Yohannes, et al 2010) LZ 1.47x10 4 >10 11.1 + ~ 3.7 0.5 (Fritz, et al 1995;Szymańska and Ślósarek 2012) FITC-DEX 4000 50 ø ~ 2.8 1 (Sigma-Aldrich) † 40.7 mg/ml solubility at pH 7 was used for the calculation of diffusion coefficients of KETO with the Hiquchi equation. Excess KETO remained in the crystalline form in the hydrogel at 3.4%, and therefore in practice initial drug concentration ˃ drug solubility.…”