Light induced expression of gRNA allows for optogenetic gene editing of T lymphocytes in vivo
Diego Velasquez Pulgarin,
Nathalie Pelo,
Lin Ferrandiz
et al.
Abstract:There is currently a lack of tools capable of perturbing genes in both a precise and spatiotemporal fashion. CRISPR’s ease of use and flexibility, coupled with light’s unparalleled spatiotemporal resolution deliverable from a controllable source, makes optogenetic CRISPR a well-suited solution for precise spatiotemporal gene perturbations. Here we present a new optogenetic CRISPR tool, BLU-VIPR, that diverges from prevailing split-Cas design strategies and instead focuses on optogenetic regulation of gRNA prod… Show more
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