Light‐Dependent Accumulation of Tryptamine in the Rice Sekiguchi Lesion Mutant Infected with <i>Magnaporthe grisea</i>

Arase, Seiji; Ueno, Makoto; Toko, M.; Honda, Yuichi; Itoh, K.; Ozoe, Yoshihisa

doi:10.1111/j.1439-0434.2001.tb03871.x

Cited by 13 publications

(5 citation statements)

References 23 publications

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“…However, tryptamine did not accumulate in rice with blast lesion formation. These results suggested that tryptamine plays an important role in light-enhanced resistance in the rice Sekiguchi lesion mutant (Arase et al 2001). …”

Section: Introductionmentioning

confidence: 94%

Studies on the expression of light-induced resistance of plants to the rice blast fungus

Ueno

2011

J Gen Plant Pathol

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Section: Introductionmentioning

confidence: 94%

Studies on the expression of light-induced resistance of plants to the rice blast fungus

Ueno

2011

J Gen Plant Pathol

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“…72 The deficiency of serotonin in rice plants could have secondary effect on other organisms in the paddy ecosystem. In addition to the increased resistance to insect pests BPH and SSB, Arase et al 73 showed increased resistance to Magnaporthe grisea under light. In addition, Ishihara et al 74 observed that the Sekiguchi lesion mutant became more susceptible to Bipolaris oryzae infection than its parent.…”

Section: The Tradeoffs Of Ost5h Knockoutmentioning

confidence: 99%

Disruption of serotonin biosynthesis increases resistance to striped stem borer without changing innate defense response in rice

Wang

Zhang

et al. 2023

Journal of Pineal Research

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Striped stem borer (SSB) is one of the most damaging pests in rice production worldwide. Previously, we preliminarily demonstrated that indica rice Jiazhe LM, an OsT5H (encoding tryptamine-5-hydroxylase) knockout mutant deficient in serotonin, had increased resistance to SSB as compared with its wildtype parent Jiazhe B. However, the full scenario of SSB resistance and the underlying mechanism remain unknown. In this study, we first demonstrated that the OsT5H knockout could generally increase rice resistance to SSB and then proved that the OsT5H knockout does not disrupt the innate defense response of rice plants to SSB infestation, that is, OsT5H knockout mutations neither had significant effect on the transcriptional response of defense genes upon SSB infestation, nor the profile of defense related metabolites and plant hormones, such as lignin, salicylic acid, jasmonic acid, and abscisic acid, nor the activity of reactive oxygen species (ROS) scavenging enzymes and the ROS contents. We then demonstrated that supplementation of serotonin promoted SSB growth and performance in artificial diet feeding experiments. We observed that SSB larvae feeding on Jiazhe B had serotonin 1.72-to 2.30-fold that of those feeding on Jiazhe LM at the whole body level, and more than 3.31 and 1.84 times in the hemolymph and head, respectively. Further studies showed that the expression of genes involved in serotonin biosynthesis and transport was ~88.1% greater in SSB larvae feeding on Jiahze LM than those feeding on Jiazhe B. These observations indicated that SSB increases serotonin synthesis when feeding on serotonin deficient rice but is unable to fully compensate the dietary serotonin deficiency. Put together, the present study strongly suggests that it is the deficiency of serotonin, not the secondary effect of OsT5H knockout on innate defense response confers the SSB resistance in rice, which implies that reducing serotonin level, particularly through inhibition of its inductive synthesis upon SSB damage, could be an efficient strategy for breeding SSB resistant varieties.

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“…Sekiguchi-asahi was grown in a greenhouse as described previously by Arase et al (2001). Virulent strain (Naga 69-150, race 007) of M. grisea was grown on rice bran agar medium at 26°C for 14 days.…”

Section: Plant Pathogen and Inoculationmentioning

confidence: 99%

“…Previously, we isolated an indole alkaloid, tryptamine, from a Sekiguchi lesion, but not from healthy tissues, as a possible factor in the light-enhanced resistance of the rice sl mutant (Arase et al 2001). Tryptamine inhibited not only spore germination and appressorium formation by M. grisea at high concentrations ([600 lg/ml) but also infection hypha formation in onion cells at low concentrations (150-300 lg/ml).…”

Section: Introductionmentioning

confidence: 99%

Effect of glyphosate on tryptamine production and Sekiguchi lesion formation in rice infected with Magnaporthe grisea

et al. 2008

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When exposed to light, the Sekiguchi lesion (sl) rice mutant has an enhanced resistance to Magnaporthe grisea infection responsible for Sekiguchi lesion formation and tryptamine accumulation. Glyphosate [N-(phosphonomethyl) glycine] pretreatment suppressed Sekiguchi lesion formation and tryptamine accumulation in the sl mutant after M. grisea infection even under light. This inhibition by glyphosate was blocked by the supply of exogenous tryptophan, but not by exogenous phenylalanine. In glyphosate-pretreated leaves, 5-enol-pyruvylshikimate-3-phosphate synthase gene expression and tryptophan biosynthesis were significantly suppressed. During tryptophan starvation, catalase activity was maintained at a high level even under light, leading to the suppression of H 2 O 2 generation and DNA fragmentation. These results show a strong relationship between the tryptophan and tryptamine pathways in the induction of light-enhanced resistance to M. grisea infection in the sl mutant.

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Light‐Dependent Accumulation of Tryptamine in the Rice Sekiguchi Lesion Mutant Infected with Magnaporthe grisea

Cited by 13 publications

References 23 publications

Studies on the expression of light-induced resistance of plants to the rice blast fungus

Studies on the expression of light-induced resistance of plants to the rice blast fungus

Disruption of serotonin biosynthesis increases resistance to striped stem borer without changing innate defense response in rice

Effect of glyphosate on tryptamine production and Sekiguchi lesion formation in rice infected with Magnaporthe grisea

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