Light and transmission electron microscopic evaluation of the lamina epithelialis mucosae in the ileum of pre‐ and post‐hatch broiler chicken

Udoumoh, Anietie Francis; Nwaogu, Innocent Chima; Igwebuike, Udensi Maduabuchi; Obidike, Ikechukwu Reginald

doi:10.1111/ahe.12774

Cited by 2 publications

(3 citation statements)

References 37 publications

(54 reference statements)

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“…Enteroendocrine cells develop during the late embryonic stage in Nigerian indegenous chicken and occur in the ileum of the chicken at ED 19. This contrasts with a previous report in broiler chicken where enteroendocrine cells developed post‐hatch (Udoumoh et al., 2022). The positive PAS‐Alcian blue staining reactions confirm the mucin‐secreting role of goblet cells and preponderance of acidic mucin in the small intestine during the late embryonic stage of development.…”

Section: Discussioncontrasting

confidence: 99%

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Morphological features of the late‐embryo‐stage gastrointestinal tract of free‐roaming light ecotype Nigerian indigenous chicken

Udoumoh,

Igwebuike,

Nwaogu

et al. 2023

Anat Histol Embryol

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Morphological changes in the late‐embryo‐stage gastrointestinal tract of Nigerian indigenous chicken were investigated using anatomical techniques. The paraffin‐embedded sections were stained with haematoxylin and eosin and periodic acid Schiff‐Alcian blue (pH 2.5) stains. During the late pre‐hatch period, the framework of the stomach was already established, but glands were yet poorly developed. Randomly oriented pre‐glandular cells in the lamina propria mucosae at embryonic day (ED) 14 became organized into elongated simple tubular glands at ED 19 and assumed adult morphology at post‐hatch day (PD) 1. Acidic and neutral mucin deposits were associated with the glandular cells. In the small intestine, enterocytes of the tunica epithelialis mucosae transformed from cuboidal‐shaped cells (at ED 14) to tall columnar cells (at PD 1). Short crypts of Lieberkühn, goblet cells, microfold cells and enteroendocrine cells were evident at ED 19, while the lamina propria mucosae and submucosa contained mesenchymal cells, reticular cells and isolated lymphoblasts. Similarly, the crypts, lymphoblasts, mesenchymal and reticular cells were also associated with the lamina propria mucosae and submucosa of the caecum and colorectum at ED 19. It was inferred from these findings that extensive cellular and tissue modifications occur in the gastrointestinal tract within the narrow window of the late pre‐hatch period. The definitive gut tunics, epithelium, glandular tissues, immune‐competent cells and tissues are formed as a result. Thus, the embryonic gut of the bird is made capable of assuming its necessary functions of food digestion, nutrient absorption, water and ion re‐absorption, immune surveillance, antibody production and immune responses at hatch.

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Section: Discussioncontrasting

confidence: 99%

Section: Transmission Electron Microscopic Features Of the Ileumcontrasting

confidence: 99%

Morphological features of the late‐embryo‐stage gastrointestinal tract of free‐roaming light ecotype Nigerian indigenous chicken

Udoumoh,

Igwebuike,

Nwaogu

et al. 2023

Anat Histol Embryol

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“…The embedding blocks were cut into ultrathin sections, stained with uranyl acetate and lead citrate. The results were observed using a Hitachi HT-7800 TEM with 80 KV [21].…”

Section: Transmission Electron Microscopy (Tem)mentioning

confidence: 99%

Silica dust alters fecal microbiota that contributes silicosis through the lung-gut axis in rats

Qi¹,

Han²,

Jia³

et al. 2023

Preprint

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Background：Silicosis is a lung disease with diffuse nodular pulmonary fibrosis because of long-term inhalation of a large number of free silica dust. Research has been reported that dysbiosis of fecal microbiota was existed in silicosis patients. However, few studies have examined the effects of silica on the intestinal tract. Objective: In this study, we aimed to investigate the change of fecal microbiota, lung and ileum tissues of rats exposed to silica dust and explore the regulatory role of fecal microbiota in rats after silica exposure. Methods: The Wistar male rats were intratracheally instilled with 50 mg/mL silicon dioxide (1 mL per rat). Hematoxylin and eosin (HE), Masson staining, enzyme-linked immunosorbent assay (ELISA) and Western blot were used to exam the pulmonary inflammation and fibrosis in rats. HE, Western blot and Transmission Electron Microscopy (TEM) were used to exam the ileac injury. The 16s rRNA gene sequences, non-targeted metabolomics, transcriptome analysis were used to exam the fecal microbiota, metabolites and ileac mRNAs respectively. Then, we employed fecal microbiota transplantation (FMT) experiment to examine whether fecal microbiota play the important roles in the change of silica-induced pulmonary inflammation, fibrosis and ileum injury. Meanwhile, Pearson correlation tests were used to detect the differential microbiota and metabolites of feces, and mRNAs of ileum on day 56. Results: The results showed that silica exposure resulted in dynamic change of pulmonary inflammation, fibrosis, fecal microbiota dysbiosis and ileum epithelial injury. FMT up-regulated the level of Bifidobacterium, restored the level of tight junction proteins of ileum. Then we found the level of Bifidobacterium was significantly down-regulated on day 56 in silica-exposed rats. Further we mainly predicted 3 potential mechanisms through conjoint analysis and KEGG analysis: (ⅰ) the change of Bifidobacterium may be related to the production of oleoyl 3-carba cyclic phosphatidic acid (3-CCPA) and the expression of Cldn8, which involved in silica-induced pulmonary inflammatory response and ileac barrier function injury; (ⅱ) Silica-induced fecal microecological dysbiosis and inflammatory respond may affect the arginine biosynthesis and utilization of arginine by regulating the level of N-Acetyl-L-Glutamic Acid and Nos2, which effect ileac architectural integrity; and (ⅲ) Silica-induced fecal microecology disorder may induce ileac injury by regulating Ido1, Kynu and Indole-3-ethanol mediated “Tryptophan metabolism” pathway. Discussion: This study provided evidence that silica could alter fecal microbiota which may in turn play an important role in silica-induced pulmonary fibrosis and ileac barrier injury in rats. Three predicted mechanistic pathways deserved further study. The findings may provide a starting roadmap to intervene in the development of silica-induced pulmonary fibrosis.

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Light and transmission electron microscopic evaluation of the lamina epithelialis mucosae in the ileum of pre‐ and post‐hatch broiler chicken

Cited by 2 publications

References 37 publications

Morphological features of the late‐embryo‐stage gastrointestinal tract of free‐roaming light ecotype Nigerian indigenous chicken

Morphological features of the late‐embryo‐stage gastrointestinal tract of free‐roaming light ecotype Nigerian indigenous chicken

Silica dust alters fecal microbiota that contributes silicosis through the lung-gut axis in rats

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