Ligation-Induced Acute Pancreatitis in Rats and Opossums: A Comparative Morphologic Study of the Early Phase

Effect of carbon dioxide pneumoperitoneum on the severity of acute pancreatitis: An experimental study in rats

“…Common biliary and pancreatic duct ligation in rats results in transient mild to moderate acute pancreatitis [8]. Because this procedure does not induce severe pancreatitis but only mild inflammation, it reflects the pathogenesis of mild acute biliary pancreatitis and produces similarities to human pancreatitis [14]. This similarity suggests that ligation-induced pancreatitis in rats may be a useful model of mild pancreatitis in humans for which laparoscopic cholecystectomy is performed in terms of the safety of the procedure.…”

Bile and Pancreatic Enzyme Exclusion Influence Early Ligation-induced Acute Pancreatitis in Opossums

“…Based on the results of these experiments in the rat model, we performed the present experiments in the opos sum model of ligation-induced acute pancreatitis for three main reasons: (1) the opossum model is an experimental model of more severe acute pancreatitis than the rat mod el [12]; (2) there would be the advantage of testing the same principle in two different species [7], and (3) the models of ligation-induced acute pancreatitis in both spe cies are suitable experimental corollaries of gallstoneinduced acute pancreatitis in humans, and, therefore, there would be focus in the purpose of our investigations [7], In both species, we have restricted our investigations for the present time to the early phase of the development of disease, as we believe that it would be an advantage to understand the early events in greater detail before later events are investigated and elucidated [7],…”

“…The opossum model of ligation-induced acute necro tizing pancreatitis has established itself as an important experimental corollary of gallstone-induced acute pancre atitis to investigate the development of disease [1,[6][7][8][9][10][11][12][13], First described in 1984 [9], and further characterized in 1986 [1], it was shown that opossums with bile and pan creatic duct ligation develop a necrotizing form of acute Pancreatic histologic changes were assessed qualitatively using a blinded acute pancreatitis histology scoring system (see table 1 ). The values of the individual components of the scoring system have been tabulated from set 2 experiments.…”

“…1) [1,6,7]. Therefore, it is possible to occlude the bile-pancreatic ductal system without reflux of bile into the pancreatic duct by separate ligation of the bile duct and the pan creatic duct proximal to the common channel [1,6].…”

“…One section stained with hematoxylin and eosin was prepared from each cross-sectional portion of the pancreas. Each histology slide was examined in a blinded fashion to be assigned an acute pancreatitis histology score (range 0-11) based on degree of edema (range 0-1), white blood cell infiltration (range 0-4), acinar cell vacuolation (range 0-2), acinar cell necrosis (range 0-2), and pcripancreatic fat necrosis (range 0-2; table 1) [7], The plasma amylase levels were determined using a spectrophotometer (CX5: Brea, Calif., USA).…”