Ligands for mixed-mode protein chromatography: Principles, characteristics and design

Protein adsorption and chromatography on novel mixed-mode resins fabricated from butyl-modified poly(ethylenimine)-grafted Sepharose

“…Nowadays, mixed-mode chromatography (MMC), which is based on the multi-modal interactions between the stationary phase and solutes in the feedstock, such as electrostatic interaction, hydrophobic interaction, hydrogen bond, thiophilic interaction and so on, has attracted extensive interests due to its convenient usage and excellent performance in protein separation and purification (Burton et al, 1997;Gilar et al, 2008;Jandera, 2008;Yu et al, 2015;Zhao et al, 2009). Generally, the MMC adsorbents show great salt tolerance for protein binding in a wide range of ionic strengths (ISs) Liu et al, 2014a;Liu et al, 2009).…”

“…Most commercial MMC resins were obtained by this method. Besides, approaches to rational design and screening of novel mixed-mode ligand have been reviewed previously (Zhao et al, 2009), benefiting in the devolvement of MMC resins. However, the development of novel mixed-mode ligand with suitable chromatographic properties is still a difficult work, because of the requirement of the proper combination of the multiple interactions on a certain ligand.…”

Optimization of a preparative multimodal ion exchange step for purification of a potential malaria vaccine

“…The used sorbents Capto TM adhere [15,16] is a strong anion exchanger coupled with a phenyl group for hydrophobic interactions and a hydroxyl group for hydrogen bonding. The nature of the interaction depends on the specific characteristics of the target protein and the set conditions of the mobile phase during the chromatographic separation.…”

Dual salt mixtures in mixed mode chromatography with an immobilized tryptophan ligand influence the removal of aggregated monoclonal antibodies

“…The ligand carries a hydrophobic moiety and a carboxylic acid, and functions as a weak cation exchanger entity. Heterocyclic hydrophobic entities are favorable as they benefit from unique hydrophobicity and dissociation properties [8].…”