Life cycle of Amblyomma integrum (Acari: Ixodidae) under laboratory conditions

Abstract

Amblyomma integrum is a hard tick infesting mainly buffalo and cattle and has been identified as an agent of human otoacariasis in Sri Lanka. Data on the life cycle pattern of A. integrum were collected by experimental infestation on New Zealand white rabbits under laboratory conditions. Wild-caught females laid 55-7389 eggs for 2-35 days after spending a latent period of 10-25 days. Egg incubation period was 31-105 days and the newly emerged larvae started feeding after 4-11 days. Larvae dropped off after feeding and they moulted into nymphs after 10-16 days. Nymphs actively fed on rabbits for 4-8 days and dropped off. Engorged nymphs took 11-25 days for moulting before emerging as adults. The male:female sex ratio of the adults moulted under laboratory conditions was 11:9. All the stages showed periodicity in engorgement and dropping off. The three-host life cycle was completed within 74-245 days with an average of 152.9 days. The mean Reproductive Efficiency Index (REI) and Reproductive Aptitude Index (RAI) were 3.6 and 1.1, respectively. Females hatched in the laboratory did not successfully feed on New Zealand white rabbits. The wild-caught females which fed on buffaloes had prolonged pre-oviposition and oviposition periods, low REI, low RAI and low eclosion under controlled laboratory conditions compared to other tick species. Although larva and nymphs of A. integrum successfully fed on New Zealand white rabbits under laboratory conditions, full life cycle was not completed because the adult females did not feed on rabbits.

Species composition of hard ticks (Acari: Ixodidae) on domestic animals and their public health importance in Tamil Nadu, South India

“…The presence of hard ticks in different domestic animals was already reported from many states like Karnataka, Tamil Nadu and Assam (Jagannah et al, 1979;Latha et al, 2004;Miranpuri and Singh, 1978). Saxana et al (1984) reported eight different species of ticks, and Kumar et al Bandaranayaka et al (2016), R2-Ghosh and Nagar (2014), R3-Geevarghese and Mishra (2011), R4-Chochlakis et al (2014.…”

Redescription of Amblyomma integrum adults and immature stages

“…Amblyomma integrum is a three‐host species under laboratory conditions (Bhat, ; Satyanarayana et al ., ; Bandaranayaka et al ., ).…”