2010
DOI: 10.1111/j.1525-1594.2009.00885.x View full text |Buy / Rent full text
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Abstract: Various metabolic tests were compared for the performance characterization of a liver cell bioreactor as a routine function assessment of cultures in a standby for patient application in clinical studies. Everyday quality assessment (QA) is essential to ensure a continuous level of cellular functional capacity in the development of hepatic progenitor cell expansion systems providing cells for regenerative medicine research; it is also of interest to meet safety requirements in bioartificial extracorporeal live… Show more

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“…In conventional static 2-D Petri dish cultures, fewer effects on gene expression were observed. This could be explained by the known superior properties of 3-D [15][16][17][18]20,[24][25][26][27][28] cultures versus 2-D static monolayer cultures in maintaining liver-specific cell types, gene expression and functionality. [29][30][31] After knockdown of C/EBPα in 3-D perfusion culture, endothelial cell numbers were reduced, whereas hepatoblast cell numbers and their protein secretion of AFP and albumin were increased.…”
Section: Discussionmentioning
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“…In conventional static 2-D Petri dish cultures, fewer effects on gene expression were observed. This could be explained by the known superior properties of 3-D [15][16][17][18]20,[24][25][26][27][28] cultures versus 2-D static monolayer cultures in maintaining liver-specific cell types, gene expression and functionality. [29][30][31] After knockdown of C/EBPα in 3-D perfusion culture, endothelial cell numbers were reduced, whereas hepatoblast cell numbers and their protein secretion of AFP and albumin were increased.…”
Section: Discussionmentioning
“…At days 7 and 14, a low consumption of glucose as well as consumption of galactose and sorbitol as additional energy sources was observed. Hereby, the uptake of sorbitol is insulin‐independent and it was recently shown that both galactose and sorbitol consumption could be used for the assessment of cell performance in liver bioreactors (Gerlach et al , 2010b).…”
Section: Discussionmentioning
“…The GEC is a physiological measure of hepatic metabolic capacity and is part of our clinical workup of patients with cirrhosis [2,3]. We obtained the results of all GEC tests conducted during the follow-up period from our department’s laboratory database.…”
Section: Methodsmentioning
“…Cirrhosis patients have reduced hepatic metabolic capacity, and the residual capacity can be measured with the galactose elimination capacity (GEC) test [2], the result of which is a strong predictor of mortality [3]. Some patients with alcoholic cirrhosis continue drinking alcohol, and such exposure to the etiology of their disease increases mortality [4].…”
Section: Introductionmentioning