LI‐cadherin gene expression during mouse intestinal development

Abstract

LI-cadherin (Liver-Intestine cadherin) is a member of a subclass (7-D cadherins) within the cadherin superfamily. Although its cellular function as a cell-cell adhesion molecule has been demonstrated in cell culture studies, its physiological function still needs to be explored in the intact organism. After isolating the cDNA for mouse LI-cadherin, we generated specific antibodies against the overexpressed protein and studied its expression pattern in adult mouse tissues and mouse embryos. The mouse LI-cadherin sequence is 91% identical to the sequence of rat LI-cadherin and exhibits the same structural features described for rat LI-cadherin. In mouse adult tissue, LI-cadherin is expressed in the intestine and in small amounts in the spleen. In contrast to rat, Mouse LI-cadherin was not expressed in liver. During mouse embryogenesis, LI-cadherin expression begins at embryonic day 12.5. With the exception of transient expression in the urogenital sinus and the common bile duct on day 13.5, LI-cadherin was found exclusively in the intestinal epithelium. Its expression coincides with the formation of intestinal villi, a developmental stage that includes major tissue remodeling, growth, and differentiation. LI-cadherin is expressed along the entire anterior-posterior axis of the developing intestine as well as along the entire villus axis once villi begin to form. LI-cadherin occupies all cell surfaces of the deeper layers of the epithelium, distributing to basolateral surfaces only in the cells of the outer epithelial layer. LI-cadherin was found to be always coexpressed with E-cadherin.

Immunohistochemistry in Undifferentiated Neoplasm/Tumor of Uncertain Origin

“…[99][100][101][102] The main function of CDH17 is to mediate cell-cell adhesion and to act as an intestinal peptide transporter. [99][100][101][102] CDH17 is expressed in normal glandular epithelium of the GI tract and normal pancreatic ducts. 102 A few reports 101,102 found CDH17 was a highly sensitive marker for GI ADCs and neuroendocrine neoplasms.…”

Cadherin-17 and SATB2 Are Sensitive and Specific Immunomarkers for Medullary Carcinoma of the Large Intestine

“…[22][23][24][25] The main function of CDH17 is to mediate cellcell adhesion and to act as an intestinal peptide transporter. [22][23][24][25] CDH17 is expressed in normal glandular epithelium of the GI tract and normal pancreatic ducts. 25 A limited number of reports have demonstrated that CDH17 is a highly sensitive marker for GI adenocarcinomas and neuroendocrine neoplasms.…”

Unique gene structure and paralogy define the 7D-cadherin family

“…So far, the expression pattern of zebrafish Ksp-cadherin has not been analyzed. In mice, temporal LI-cadherin expression was also observed during embryogenesis in the epithelia of the urogenital sinus at day 13.5 post coitus [40]. This temporally overlapping expression pattern of Ksp-and LI-cadherin at certain developmental stages might be a relict of their common phylogentic origin.…”

“…The tissue-specific expression of today's Ksp-and LI-cadherin in highly resorptive epithelial cells of the kidney and the intestine, respectively [40,43], might have been responsible for their survival during evolution. Interestingly, the recently identified zebra fish LI-cadherin ortholog [59] shows an expression pattern that resembles both mouse Ksp-and LI-cadherin.…”

“…Neither Ksp-nor LI-cadherin contain an N-terminal propeptide, which is a characteristic feature of immature classical cadherins [38]. Interestingly, Ksp-and LI-cadherin are each coexpressed with E-cadherin and evenly distributed along the lateral contact areas of highly resorptive polarized epithelia of the kidney or the intestine, respectively [35,39,40]. These similar structural and functional features of Ksp-and LI-cadherin suggest a common phylogenetic origin of both molecules.…”