Levels of the Native Forms of GnRH in the Pituitary of the Gilthead Seabream,Sparus aurata, at Several Characteristic Stages of the Gonadal Cycle

Holland, M. Claire H.; Gothilf, Yoav; Meiri, Iris; King, Judy A.; Okuzawa, Koichi; Elizur, Abigail; Zohar, Yonathan

doi:10.1006/gcen.1998.7138

Cited by 103 publications

(62 citation statements)

References 30 publications

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“…During the last two decades, many studies have described aspects of the biology of the species, including reproduction and genetics (Holland et al, 1998;Almansa et al, 1999;Meiri et al, 2004;Rossi et al, 2006;Arabaci et al, 2010;Mylonas et al, 2011). Gilthead seabream is a protandrous hermaphrodite species with an asynchronous ovarian development (Zohar et al, 1995).…”

Section: Introductionmentioning

confidence: 99%

Mating behaviour and gamete release in gilthead seabream (Sparus aurata, Linnaeus 1758) held in captivity

Ibarra-Zatarain

Duncan

2015

Span. j. agric. res.

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The present study aimed to describe the reproductive behaviour of gilthead seabream (Sparus aurata) in captivity. Twenty-four mature gilthead seabream, divided in two tanks, were utilized for the present study. Reproductive behaviour was recorded using submersibles cameras. A total of 67 spawning events were analysed. The mean duration time that gilthead seabream spent spawning was 54 ± 4 min/day, during which mean number of individual spawning events was 5.6 ± 0.2. The mean volume of eggs produced by both broodstocks was 405 ± 13.4 mL with a fertilization rate of 91.6 ± 0.4%. The reproductive behaviour began with a schooling behaviour and then forming light aggregations. From an aggregation or an encounter while swimming freely a female initiated a spawning rush followed by one or more males to gametes liberation. The spawning rush was brief, 1.6 ± 0.5 sec, over an approximately 1.7 ± 0.2 m distance from the tank bottom to the water surface. Pair spawning, between a single female and male, was the most common (71.6%). Group spawning was less common and involved a single female spawning with two males (22.5%) or three males (4.9%). Spawning rushes involving more than one female were not observed. Gilthead seabream in the present study presented a tendency to pair spawn and eggs collected as a "spawn" were actually the sum of many separate spawning events over a short time period. This is the first description of gilthead seabream spawning and the findings help to understand microsatellite based observations of spawning kinetics. Additional key words: mate selection; pair group mass spawning; rush; aggregation Citation: Ibarra-Zatarain, Z.; Duncan, N. (2015). Mating behaviour and gamete release in gilthead seabream (Sparus aurata,

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Section: Introductionmentioning

confidence: 99%

Mating behaviour and gamete release in gilthead seabream (Sparus aurata, Linnaeus 1758) held in captivity

Ibarra-Zatarain

Duncan

2015

Span. j. agric. res.

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“…Interestingly, Gnrh1 has been identified as the most relevant Gnrh form controlling oocyte development and reproductive activity in several fish species (Holland et al 1998, Andersson et al 2001, Zmora et al 2002, Okuzawa et al 2003, Molés et al 2007). Previous studies have reported overlapping expression of gnrhr2a with both fshb and lhb in pituitary cells of European sea bass (González-Martínez et al 2004), findings supporting a putative influence of Gnrh-derived signaling on gonadotropin subunits transcription and gonadotropin release.…”

Section: Endocrine Responses To Ulomentioning

confidence: 99%

Mechanisms of oocyte development in European sea bass (Dicentrarchus labrax L.): investigations via application of unilateral ovariectomy

García-López¹,

Sánchez-Amaya²,

Tyler³

et al. 2011

Reproduction

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Unilateral ovariectomy (ULO) was performed in European sea bass (Dicentrarchus labrax L.) during late pre-vitellogenesis/early vitellogenesis. Plasma steroid levels and the expression of a suite of potential oogenesis-relevant genes in the ovary, brain, and pituitary were evaluated with the aim of understanding their involvement in the compensatory oocyte development occurring within the remaining ovarian lobe. After 69 days of surgery the remaining ovarian lobe in ULO fish was gravimetrically equivalent to an intactpaired ovary of sham operated, control fish. This compensatory ovarian growth was based on an increased number of early perinucleolar oocytes and mid-late stage vitellogenic follicles without an apparent recruitment of primary oocytes into the secondary growth phase. Plasma steroid levels were similar in ULO and control females at all time points analyzed, suggesting an increased steroid production of the remaining ovarian lobe in hemi-castrated females. Results of the gene expression survey conducted indicate that the signaling pathways mediated by Fsh and Gnrh1 constitute the central axes orchestrating the observed ovarian compensatory growth. In addition, steroid receptors, Star protein, Igfs, and members of the transforming growth factor beta superfamily including anti-Mullerian hormone and bone morphogenetic protein 4 were identified as potentially relevant players within this process, although their specific actions and interactions remain to be established. Our results demonstrate that ULO provides an excellent in vivo model for elucidating the interconnected endocrine and molecular mechanisms controlling oocyte development in European sea bass.

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“…After centrifugation (13000 g for 30 min at 7 4ºC), the supernatants were dried in a speed vacuum and reconstituted in EIA buffer for analysis. 8 The pituitary sbGnRH content was measured in reconstituted samples using a competitive enzyme-9 linked immunosorbent assay (ELISA) similar to that described by Holland et al (1998), which was 10 modified for sea bass. The sensitivity of the assay was 6 pg/well, and cross-reactivity with cII and 11 sGnRH was calculated to be 0.6 and 0.4%, respectively.…”

mentioning

confidence: 99%

Effects of Continuous Light on the Reproductive System of European Sea Bass Gauged by Alterations of Circadian Variations during Their First Reproductive Cycle

Bayarri

Zanuy

Yilmaz

et al. 2009

Chronobiology International

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Levels of the Native Forms of GnRH in the Pituitary of the Gilthead Seabream,Sparus aurata, at Several Characteristic Stages of the Gonadal Cycle

Cited by 103 publications

References 30 publications

Mating behaviour and gamete release in gilthead seabream (Sparus aurata, Linnaeus 1758) held in captivity

Mating behaviour and gamete release in gilthead seabream (Sparus aurata, Linnaeus 1758) held in captivity

Mechanisms of oocyte development in European sea bass (Dicentrarchus labrax L.): investigations via application of unilateral ovariectomy

Effects of Continuous Light on the Reproductive System of European Sea Bass Gauged by Alterations of Circadian Variations during Their First Reproductive Cycle

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