Levels of matrix metalloproteinases 1 and 2 in human gingival crevicular fluid during initial tooth movement

Cantarella, Giuseppina; Cantarella, Rosita; Caltabiano, M; Risuglia, Nunziata; Bernardini, Renato; Leonardi, Rosalia

doi:10.1016/j.ajodo.2006.04.020

Cited by 50 publications

(49 citation statements)

References 25 publications

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“…25 In the current study, the lower limit of detection for the MMPs examined ranged from 11 pg/mL for MMP-1 to 102 pg/mL for MMP-7. All MMPs examined, including MMP-1, were detected by the bead immunoassay in the majority of samples analyzed, while previous studies [17][18][19] failed to detect MMP-1 in GCF samples. Data reported for MMP-8 using immunofluorometric assays suggested a lower limit of detection of approximately 1 ng/mL.…”

Section: Discussionmentioning

confidence: 60%

“…Previous research [17][18][19][20] has indicated that GCF levels of MMPs and activation of collagenases can be altered by orthodontic forces. Apajalahti et al 19 and Ingman et al 18 found that using an immunofluorometric assay significantly elevated MMP-8 immunoreactivity in GCF samples collected after force application.…”

Section: Discussionmentioning

confidence: 99%

“…Those findings are in agreement with previous results 20 that have shown significantly elevated total collagenase activity in the GCF of orthodontic patients 24 hours after retractor activation. Western blotting analysis was also used by Cantarella et al 17 to examine the expression of MMP-1 and -2 in GCF samples from the compression and tension sides during force application. The levels of MMP-1 increased after 1 hour of activation of the orthodontic device in both sides, which was also found in this study.…”

Section: Discussionmentioning

confidence: 99%

“…Our study examined treated periodontitis subjects under maintenance, while other authors [17][18][19]24 studied periodontally healthy individuals. However, using multiplex bead immunoassay we have also examined the effects of orthodontic forces on periodontally healthy teeth and found MMPs changes for the compression sites but not for the tension sites as a result of mechanical forces.…”

Section: Discussionmentioning

confidence: 99%

“…14 Orthodontic tooth movement can be delayed or prevented in mice and rats by the use of MMP inhibitors. 15,16 A few human studies [17][18][19] have quantified the presence of MMPs in GCF during orthodontic tooth movement and have reported alterations in their levels during the application of orthodontic forces. Further, total collagenase activity in the GCF of orthodontic patients treated with fixed appliances has been shown to be 10-fold that of control GCF.…”

Section: Introductionmentioning

confidence: 99%

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Levels of gingival crevicular fluid matrix metalloproteinases in periodontally compromised teeth under orthodontic forces

Almeida

Capelli

Teles³

2015

The Angle Orthodontist

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Objective: To examine levels of matrix metalloproteinases (MMPs)-1, -2, -3, -7, -8, -12, and -13 in the gingival crevicular fluid (GCF) of periodontally compromised teeth at different time points during orthodontic movement. Materials and Methods: Ten controlled periodontitis subjects were submitted to orthodontic treatment. One dental arch was subjected to orthodontic movement, and teeth in the opposite arch were used as controls. GCF samples were collected from the lingual sites of two movement and two control incisors 1 week before orthodontic activation (27 d), immediately after orthodontic activation, and after 1 hour, 24 hours, and 7, 14, and 21 days. Multiplexed bead immunoassay was used to measure MMPs in GCF. Data were analyzed using Friedman and Wilcoxon statistical tests. Results: The only significant change found over time was in the levels of MMP-1 in the movement group (P , .05). When the two groups were compared after activation, the only statistically significant difference found was in levels of MMP-12 24 hours after activation (P , .05). Conclusions: Our findings suggested that the orthodontic movement of periodontally compromised teeth without active pockets did not result in significant changes in the GCF levels of MMPs.

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Section: Discussionmentioning

confidence: 60%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Levels of gingival crevicular fluid matrix metalloproteinases in periodontally compromised teeth under orthodontic forces

Almeida

Capelli

Teles³

2015

The Angle Orthodontist

View full text Add to dashboard Cite

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Highly variable rate of orthodontic tooth movement measured by a novel 3D method correlates with gingival inflammation

et al. 2020

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Objectives Individual orthodontic treatment duration is hard to predict. Individual biological factors are amongst factors influencing individual rate of orthodontically induced tooth movement (OTM). The study aim is to determine the rate of OTM by a novel 3D method and investigate parameters that may predict the rate of tooth movement. Materials and methods In this prospective cohort study, rate of OTM was determined from 90 three-dimensional intra-oral scans in 15 patients (aged 12–15) undergoing orthodontic treatment. For each patient, intra-oral scans were taken every week for up to 6 weeks (T0–T5). The teeth were segmented from the scans and the scans were superimposed on the palatal rugae. The rate of OTM was calculated for each tooth. Other parameters were gingival inflammation, contact-point displacement and the biological markers, matrix metalloproteinases (MMP), MMP-9 and MMP-2 in gingival crevicular fluid (GCF). Results Our study showed a high variation in the rate of OTM, varying from 0.15 to 1.24 mm/week. Teeth in the anterior segment tended to move more compared with the posterior segment. The contact point displacement and gingival inflammation varied greatly amongst the patients. The MMPs measured did not correlate with tooth movement. However, the gingival inflammation index showed a significant correlation with OTM. Future studies should include other biological markers related to bone-remodeling. Conclusion This novel and efficient 3D method is suitable for measuring OTM and showed large individual variation in rate of OTM. Clinical relevance Patients show different rates of OTM. The rate of OTM in an individual patient can provide guidance in timing of follow-up appointments.

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Expression of matrix metalloproteinases-2 and -9 and RECK during alveolar bone regeneration in rat

et al. 2007

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MMPs are endopeptidases that play a pivotal role in ECM turnover. RECK is a single membrane-anchored MMP-regulator. Here, we evaluated the temporal and spatial expression of MMP-2, MMP-9, and RECK during alveolar bone regeneration. The maxillary central incisor of Wistar rats was extracted and the animals were killed at 1, 3, 7, 10, 14, 21, 28, and 42 days post-operatively (n = 3/period). The hemimaxillae were collected, demineralized and embedded in paraffin. Immunohistochemical analysis was performed by the immunoperoxidase technique with polyclonal antibodies. On day 1, polymorphonuclear cells in the blood clot presented mild immunolabeling for MMPs. During bone remodeling, osteoblasts facing new bone showed positive staining for gelatinases and RECK in all experimental periods. MMPs were also found in the connective tissue and endothelial cells. Our results show for the first time that inactive and/or active forms of MMP-2, MMP-9 and RECK are differentially expressed by osteogenic and connective cells during several events of alveolar bone regeneration. This may be important for the replacement of the blood clot by connective tissue, and in the formation, maturation and remodeling of new bone.

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Levels of matrix metalloproteinases 1 and 2 in human gingival crevicular fluid during initial tooth movement

Cited by 50 publications

References 25 publications

Levels of gingival crevicular fluid matrix metalloproteinases in periodontally compromised teeth under orthodontic forces

Levels of gingival crevicular fluid matrix metalloproteinases in periodontally compromised teeth under orthodontic forces

Highly variable rate of orthodontic tooth movement measured by a novel 3D method correlates with gingival inflammation

Expression of matrix metalloproteinases-2 and -9 and RECK during alveolar bone regeneration in rat

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