Levels of Apelin-12, AT1R, and AGT are correlated with degree of renal fibrosis in patients with immunoglobulin A nephropathy

Abstract

Background: To explore the relationship between the degree of renal fibrosis in patients with immunoglobulin A nephropathy (IgAN) and their levels of Apelin-12, Average Optical Density of angiotensin II type 1 receptor (AOD AT1R ), and angiotensinogen (AGT).Methods: A total of 156 patients with IgAN diagnosed by renal biopsy in our hospital were selected and divided into a T 0 group (54 cases), T 1 group (49 cases) and T 2 group (53 cases). The levels of Apelin-12, AT1R, and AGT were compared among the three groups, and the relationship between the above three indicators and degree of renal fibrosis was analyzed among patients with IgAN. Results:The AOD AT1R and AGT level in the T 2 group and T 1 groups were significantly higher than those of the T 0 group, and the Apelin-12 level of patients in the T 2 group and T 1 groups were significantly lower than that in T 0 group. Significances of the same trend were observed among all the above indicators between the T 2 group and T 1 group. ROC curves showed that when the cutoff value of Apelin-12 was 2.36 μg/L, the area under curve (AUC), sensitivity, and specificity of T 0 -T 1 T 2 were 0.889, 92.00%, and 88.00%, respectively.When the cut-off value of AOD AT1R was 0.065, the AUC, sensitivity, and specificity were 0.706, 76.00%, and 76.00%, respectively, and when the cut-off value of AGT was 47.26 ng/mL, the AUC, sensitivity, and specificity were 0.899, 84.00%, and 88.00%, respectively. When the cutoff value of Apelin-12 was 0.92 μg/L, the AUC, sensitivity, and specificity of T 0 T 1 -T 2 were 0.819, 84.62%, and 87.50%, respectively, and when the cutoff value of AOD AT1R was 0.079, the AUC, sensitivity, and specificity were 0.699, 76.92%, and 79.17%, respectively. When the cut-off value of AGT was 92.96 ng/mL, the AUC, sensitivity, and specificity were 0.893, 84.62%, and 91.67%, respectively.Conclusions: Apelin-12 decreased with disease progression, while AT1R and AGT increased. The changes of levels of Apelin-12, AT1R, and AGT have certain significance in judging the degree of renal fibrosis in patients with IgA nephropathy, and the change of level of AGT has the highest correlation with the degree of renal fibrosis.

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“…Angiotensinogen (AGT) is the key point of RAS system, and AGT levels influence RAS activation. Related to glomerular and renal tubular injury, AGT has the highest correlation with the degree of renal fibrosis in IgAN patients ( 30 ). The possible mechanism is that AGT can increase inflammatory factors and reactive oxidation factors via angiotensin II, induce the formation of crescent in renal tissue and aggravate the degree of renal fibrosis ( 30 ).…”

“…Related to glomerular and renal tubular injury, AGT has the highest correlation with the degree of renal fibrosis in IgAN patients ( 30 ). The possible mechanism is that AGT can increase inflammatory factors and reactive oxidation factors via angiotensin II, induce the formation of crescent in renal tissue and aggravate the degree of renal fibrosis ( 30 ). In short, many protein, physiological processes and pathways in enrichment analysis have been confirmed in recent studies, which are related to the occurrence and progress of nephropathy.…”