Levels of 8-hydroxydeoxyguanosine as a marker of DNA damage in human leukocytes

Lodovici, Maura; Casalini, Claudia; Cariaggi, Roberta; Michelucci, Lucia; Dolara, Piero

doi:10.1016/s0891-5849(99)00194-x

Cited by 127 publications

(86 citation statements)

References 21 publications

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“…Frozen lung samples were thawed at room temperature, and cell DNA isolation was performed as previously described [34] with minor modifications. The samples were homogenized in 1 ml of 10 mM PBS, pH 7.4, sonicated on ice for 1 min, added to 1 ml of 10 mM Tris-HCl buffer, pH 8, containing 10 mM EDTA, 10 mM NaCl, and 0.5% SDS, and incubated for 1 h at 37°C…”

Section: Determination Of 8-hydroxy-2'-deoxyguanosine (8ohdg)mentioning

confidence: 99%

Role of histamine H 4 receptor ligands in bleomycin-induced pulmonary fibrosis

Lucarini

Pini

Rosa

et al. 2016

Pharmacological Research

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Use policyThe full-text may be used and/or reproduced, and given to third parties in any format or medium, without prior permission or charge, for personal research or study, educational, or not-for-prot purposes provided that:• a full bibliographic reference is made to the original source • a link is made to the metadata record in DRO • the full-text is not changed in any way The full-text must not be sold in any format or medium without the formal permission of the copyright holders.Please consult the full DRO policy for further details. This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. The results here reported clearly demonstrated that H 4 R ligands have a beneficial effect in a model of lung fibrosis in the mouse, thus indicating that H 4 R antagonists or inverse agonists could be a novel therapeutic strategy for lung inflammatory diseases.4

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Section: Determination Of 8-hydroxy-2'-deoxyguanosine (8ohdg)mentioning

confidence: 99%

Role of histamine H 4 receptor ligands in bleomycin-induced pulmonary fibrosis

Lucarini

Pini

Rosa

et al. 2016

Pharmacological Research

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“…Ischemia/reperfusion is believed to provoke neuronal damage by increasing production of ROS. To evaluate the level of ROS after ischemic insult, we used 8-oxoguanine (8-oxoG), an oxidized by-product of purine base of DNA, as an indicator of oxidative damage (29). We performed a preliminary time course and observed that 8-oxoG reactivity in tissue reaches its maximum at 48 h after ischemia (data not shown).…”

Section: Dj-1-mediated Protection Is Associated With Reduced Markers Ofmentioning

confidence: 99%

The Parkinson's disease gene DJ-1 is also a key regulator of stroke-induced damage

Aleyasin

Rousseaux

Phillips

et al. 2007

Proc. Natl. Acad. Sci. U.S.A.

147

120

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Recent evidence has indicated that common mechanisms play roles among multiple neurological diseases. However, the specifics of these pathways are not completely understood. Stroke is caused by the interruption of blood flow to the brain, and cumulative evidence supports the critical role of oxidative stress in the ensuing neuronal death process. DJ-1 (PARK7) has been identified as the gene linked to early-onset familial Parkinson's disease. Currently, our work also shows that DJ-1 is central to death in both in vitro and in vivo models of stroke. Loss of DJ-1 increases the sensitivity to excitotoxicity and ischemia, whereas expression of DJ-1 can reverse this sensitivity and indeed provide further protection. Importantly, DJ-1 expression decreases markers of oxidative stress after stroke insult in vivo, suggesting that DJ-1 protects through alleviation of oxidative stress. Consistent with this finding, we demonstrate the essential role of the oxidation-sensitive cysteine-106 residue in the neuroprotective activity of DJ-1 after stroke. Our work provides an important example of how a gene seemingly specific for one disease, in this case Parkinson's disease, also appears to be central in other neuropathological conditions such as stroke. It also highlights the important commonalities among differing neuropathologies.neurodegeneration ͉ oxidative stress ͉ ischemia ͉ neuroprotection

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“…Alcohol consumption was associated with increased DNA damage or induction of 8oxoG in some studies [30,31] in contrast, but not in others [32,33] . The present study showed that alcohol intake did not increase the risk of colon cancer even in Cys/Cys carriers.…”

mentioning

confidence: 94%

hOGG1 Ser326Cys polymorphism modifies the significance of the environmental risk factor for colon cancer

Kim

Park²,

Kim³

et al. 2003

WJG

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AIM:To determine the association of hOGG1 (8-oxoguanine glycosylase I, OGG1) polymorphism of Ser326Cys substitution with colon cancer risk and possible interaction with known environmental risk factors. METHODS:A case-control study with 125 colon cancer cases and 247 controls was conducted. RESULTS:There was no major difference in Ser326Cys genotype distribution between cases and controls. The meat intake tended to increase the odds ratio for colon cancer with an OR of 1.72 (95 % confidence interval; CI=1.12-2.76). Such tendency was more prominent in Cys/Cys carriers (OR=4.31, 95 % CI=1.64-11.48), but meat intake was not a significant risk factor for colon cancer in Ser/Ser or Ser/ Cys carriers. The OR for colon cancer was elevated with marginal significance in smokers who were Cys/Cys carriers (OR=2.75, 95 % CI=1.07-7.53) but not in Ser/Ser or Ser/ Cys carriers. CONCLUSION:These results suggest that the hOGG1 Ser326Cys polymorphism is probably not a major contributor to individual colon cancer susceptibility overall, but the Cys/ Cys genotype may alter the impact of some environmental factors on colon cancer development.Kim JI, Park YJ, Kim KH, Kim JI, Song BJ, Lee MS, Kim CN, Chang SH. hOGG1 Ser326Cys polymorphism modifies the significance of the environmental risk factor for colon cancer. World J Gastroenterol 2003; 9(5): 956-960

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Levels of 8-hydroxydeoxyguanosine as a marker of DNA damage in human leukocytes

Cited by 127 publications

References 21 publications

Role of histamine H 4 receptor ligands in bleomycin-induced pulmonary fibrosis

Role of histamine H 4 receptor ligands in bleomycin-induced pulmonary fibrosis

The Parkinson's disease gene DJ-1 is also a key regulator of stroke-induced damage

hOGG1 Ser326Cys polymorphism modifies the significance of the environmental risk factor for colon cancer

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