Level and activities of antioxidants in intestine of larvae Galleria mellonella L. (Lepidoptera, Pyralidae) at peroral infestation by bacteria Bacillus thuringiensis ssp. galleriae

Дубовский, И. М.; Olifirenko, O. A.; Глупов, В. В.

doi:10.1007/s10893-005-0030-6

Cited by 12 publications

(7 citation statements)

References 24 publications

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“…Increase in oxidative stress includes the formation of free radicals, and antioxidant enzymes were involved in clearing the free radicals, so the activity increased. Bt infection increased the level of oxidative stress in the Galleria mellonella larval midgut [41,42], and higher SOD activities were observed in Bt-fed Spodoptera exigua than in non-Bt fed individuals [43]. However, there was not that much increase in activities of antioxidant in both antibiotic-and Bt toxin-fed larvae, indicating that, in microbes, eliminated H. armigera larvae Bt did not induced oxidative stress.…”

Section: Discussionmentioning

confidence: 92%

Elimination of Gut Microbes with Antibiotics Confers Resistance to Bacillus thuringiensis Toxin Proteins in Helicoverpa armigera (Hubner)

Visweshwar

Sharma

Akbar

et al. 2015

Appl Biochem Biotechnol

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Helicoverpa armigera is one of the most important pests worldwide. Transgenic crops with toxin genes from Bacillus thuringiensis (Bt) have been deployed on a large scale to control this pest. The insecticidal activity of Bt is probably influenced by the insect midgut microbes, which vary across crop hosts and locations. Therefore, we examined the role of gut microbes in pathogenicity of Bt toxins in the H. armigera. Antibiotic cocktail was used for the complete elimination of the H. armigera gut microbes. Activated Cry1Ac, Bt formulation, and transgenic cotton resulted in larval weight loss and increase in mortality, but pretreatment of larvae with antibiotic cocktail significantly decreased larval mortality and increased the larval weight gain. Activated Cry1Ac and Bt formulation inhibited the activity of proteases in midgut of H. armigera larvae but showed no such effect in the larvae pretreated with antibiotic cocktail. Five protease bands in activated Cry1Ac and two in Bt formulation-treated larvae were inhibited but no such effect in the larvae pretreated with antibiotic cocktail. Cry1Ac protein was detected in Bt/Cry1Ac protoxin-fed larval gut extract in the absence of antibiotic cocktail, but fewer in larvae pretreated with antibiotic cocktail. The activity of antioxidant enzymes and aminopeptidases increased in larvae fed on Bt toxin, but there was no significant increase in antioxidant enzymes in larvae reared on toxin protein in combination with antibiotic cocktail. The results suggest that gut microbes exercise a significant influence on the toxicity of Cry1Ac and Bt formulation in H. armigera larvae. The implications of these results have been discussed in relation to development of insect resistance to Bt transgenic crops deployed for pest management.

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Section: Discussionmentioning

confidence: 92%

Elimination of Gut Microbes with Antibiotics Confers Resistance to Bacillus thuringiensis Toxin Proteins in Helicoverpa armigera (Hubner)

Visweshwar

Sharma

Akbar

et al. 2015

Appl Biochem Biotechnol

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“…These molecules are increased when the exposure to pathogens and damage the cell structure [ [13] , [14] , [15] ]. In many studies, the antioxidant system shows a defense mechanism against ROS production induced by pathogens [ 16 , 17 ]. The major antioxidant enzymes in insects are catalase, glutathione-S-transferase, superoxide dismutases, peroxidases, and esterase [ [18] , [19] , [20] , [21] , [22] ].…”

Section: Introductionmentioning

confidence: 99%

Effect of food plants on Spodoptera litura (Lepidoptera: Noctuidae) larvae immune and antioxidant properties in response to Bacillus thuringiensis infection

2020

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The larvae of Spodoptera litura (Fabricius) were reared on five host plants, Brassica oleracea , Nicotiana tabacum , Ricinus communis , Gossypium hirsutum , and Arachis hypogaea . The larvae were immunized with Bacillus thuringiensis to observe the immune response. The results of total and differential hemocyte count were increased in B. oleracea , N. tabacum , and R. communis fed S. litura larval hemolymph. Similar results were observed in the parameter of nodulation, melanization, and phenoloxidase. Total protein was higher in R. communis fed larvae. Antioxidant levels like Catalase (CAT), Superoxide dismutase (SOD), Glutathione S- transferase (GST), Peroxidase (POX), Lipid peroxidase (LPO), and Esterase (EST) was found in moreover all plant-feeding insect. High CAT activity was observed 2–6 h in R. communis , G. hirsutum , and A. hypogaea fed S. litura larval midgut and fatbody samples. Increased SOD activity in both midgut and fatbody at 2–12 h of B. oleracea , G. hirsutum , and A. hypogaea fed. GST activity was increased initially 2−6 h in G. hirsutum and A. hypogaea . Increased POX activity was observed initially in all treated groups. Highest LPO observed at 6 h in N. tabacum in both midgut and fatbody. Whereas increased EST activity was observed in N. tabacum and B. oleracea . The results of the present study shows that nature of food influence the immunity against Bt infection. This information can be very useful for incorporating biological control program for insect pest.

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“…Insecticides may induce oxidative stress leading to a generation of free radicals and alterations in antioxidants or reactive oxygen species (ROS)scavenging enzymes in vivo and in vitro (Bagchi et al, 1995;Gultekin et al, 2000). It was reported that pesticides effected on antioxidant enzyme activities in insects (Dubovskii et al, 2005;Dubovskiy et al, 2008). In this study a change in SOD and CAT activities was found in insects' tissues homogenates after application of the Radiant SC % different concentrations.…”

Section: Groupsmentioning

confidence: 71%

“…In animals, including insects, various important components of the antioxidant system are identified. They are divided into enzymatic antioxidants glutathione peroxidases (GPx) (Dubovskii et al, 2005).…”

mentioning

confidence: 99%

Effectiveness of Radiant SC 12% on Glutathione Peroxidase Activities in Storage Pest Rice weevil, Sitophilus oryzae L. (Coleoptera: Curculionidae)

Al-Barty¹

2014

Biosci., Biotechnol. Res. Asia

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Radiant SC12% (Spinetoram) is a new generation of spinosyn group. Evaluated the efficacy of different concentrations of spinosad against adults of the rice weevil, Sitophilus oryzae were studied in the laboratory. Antioxidant defense components protect insects by scavenging reactive oxygen species, leading to oxidative stress. The present study was investigated the effects of Radiant SC 12%, on the GPX activity in Sitophilus oryzae tissues. There were statistically significant increases in GPX activity in the highest concentration of Radiant-treated Sitophilus oryzae compared to the control. These results indicated that Radiant causes an increase in oxidative stress thus increasing GPX activities.

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Level and activities of antioxidants in intestine of larvae Galleria mellonella L. (Lepidoptera, Pyralidae) at peroral infestation by bacteria Bacillus thuringiensis ssp. galleriae

Cited by 12 publications

References 24 publications

Elimination of Gut Microbes with Antibiotics Confers Resistance to Bacillus thuringiensis Toxin Proteins in Helicoverpa armigera (Hubner)

Elimination of Gut Microbes with Antibiotics Confers Resistance to Bacillus thuringiensis Toxin Proteins in Helicoverpa armigera (Hubner)

Effect of food plants on Spodoptera litura (Lepidoptera: Noctuidae) larvae immune and antioxidant properties in response to Bacillus thuringiensis infection

Effectiveness of Radiant SC 12% on Glutathione Peroxidase Activities in Storage Pest Rice weevil, Sitophilus oryzae L. (Coleoptera: Curculionidae)

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