Levantamento da incidência de Leifsonia xyli subsp. xyli em plantios de cana-de-açúcar do noroeste do Paraná

Marcuz, Fernanda Santos; Souto, Eliezer Rodrigues de; Vieira, Rafael Augusto; Marrafon, Marco Aurélio; Barboza, Antônio Augusto Lazarini; Daros, Edelclaiton

doi:10.1590/s1413-70542009000700041

Cited by 2 publications

(1 citation statement)

References 8 publications

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“…Few studies have been conducted regarding PCR feasibility directly from vascular fluid over the years Comstock and Irey [4] Lopes et al [15] Wang et al [7]. Results from this work shows that fast methods of DNA extraction, which took approximately 2 hours for a total of 100 samples, may be as effective as more time-demanding and elaborated protocols that require around 24 hours to complete [16][17][18][19][20]. Therefore, large scale diagnosis of leaf scald in sugarcane may be performed through the DNA extraction techniques M2, M4 or M5, since these methods are fast, simple and do not use phenol as one of its reagents [21][22][23].…”

Section: Resultsmentioning

confidence: 99%

Comparison of Five DNA Extraction Methods for Detection of Leaf Scald in Sugarcane Through PCR

Dias¹,

Rocha

Dianese

et al. 2019

CIACR

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Among the techniques used for Leaf Scald diagnosis, PCR is considered the most sensitive, however this technique has limitations for use in large-scale analysis, particularly because of the difficulty/complexity associated with DNA extraction. Detection success largely depends upon the host species and the protocol used for DNA extraction. Here we describe a comparison of five DNA extraction methods and determine which one is more efficient for large scale detection of Leaf Scald through PCR. DNA extraction was performed from: a. Bacterial culture; b. Bacterial culture added to the vascular fluid from sugarcane; and c. From infected plant stalks. Of the five methods tested, three were effective for large-scale detection by PCR.

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