Among the techniques used for Leaf Scald diagnosis, PCR is considered the most sensitive, however this technique has limitations for use in large-scale analysis, particularly because of the difficulty/complexity associated with DNA extraction. Detection success largely depends upon the host species and the protocol used for DNA extraction. Here we describe a comparison of five DNA extraction methods and determine which one is more efficient for large scale detection of Leaf Scald through PCR. DNA extraction was performed from: a. Bacterial culture; b. Bacterial culture added to the vascular fluid from sugarcane; and c. From infected plant stalks. Of the five methods tested, three were effective for large-scale detection by PCR.